不同苗龄接穗的西瓜嫁接体愈合过程中的3种酶活性变化

用贴接法嫁接西瓜品种早佳(8424)和砧木葫芦品种将军的结果表明:接穗苗龄越大,嫁接成活率越低.多酚氧化酶(PPO)活性嫁接初期较高,以后变化平稳;大苗龄接穗嫁接体的PPO活性较高.嫁接初期过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性升高,以后呈下降趋势,木质素合成和维管组织分化阶段再度升高.     

芸苔属植物CAL基因的结构特征与花球的形态遗传

Cauliflower curd is composed structurally of a number of shortened shoots on which there are tremendous amount of naked apical meristems. The authors isolated four pure lines of different curd types from cauliflower (Brassica oleracea L. var. botrytis) accessions: smooth curd (curd-s), haired curd (curd-h), crystal curd (curd-c) and thorned curd (curd-t). BobCAL genes was cloned separately from each pure line by PCR amplification and a termination mutation from AAG to TAG was found in the fifth exon of all four sequences, whereas DNA sequences of 638 bp upstream of the stop codons were identical, demonstrating that BobCAL is not the sole regulator of flower buds and cauline on curd. After crosses of cauliflower with cabbage (B.oleracea var. capitata) or broccoli (B.oleracea var. italica), the resultant F1 plants failed to form curd and had the diversity of inflorescence which implicates the relatedness of CAL to other regulators. One homologous gene of CAL, BcpCAL was isolated from Chinese cabbage (B.campestris L. ssp. pekinensis) and it was found not to have the termination mutation in the fifth exon, and thus the structure of BcpCAL gene was analyzed. The results offer molecular and genetic evidence for the study of biological function of CAL and morphological genetics of curd.     

大蒜贮藏叶再生鳞茎

植物名称:大蒜(Allium sativum)。材料类别:贮藏叶。培养条件:取已萌动的“山东苍山”大蒜,剥去外层保护叶后在自来水下冲洗3～5min。用70％酒精消毒30s,再用0.1％的升汞消毒15min,无菌水冲洗3～4次。在超净工作台上先横切下贮藏叶底部0.5cm长的小块,中间可见嫩芽,去除基部坏死部分,纵向均等切成6～8块,每块带少量茎盘和发芽嫩叶,以MS为基本培养基,附加不同激素(浓度单位mg/L),组合包括(1)NAA0.005 KT1.0;