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1.
Single-stranded DNA binding proteins (SSBs) selectively bind single-stranded DNA (ssDNA) and facilitate recruitment of additional proteins and enzymes to their sites of action on DNA. SSB can also locally diffuse on ssDNA, which allows it to quickly reposition itself while remaining bound to ssDNA. In this work, we used a hybrid instrument that combines single-molecule fluorescence and force spectroscopy to directly visualize the movement of Escherichia coli SSB on long polymeric ssDNA. Long ssDNA was synthesized without secondary structure that can hinder quantitative analysis of SSB movement. The apparent diffusion coefficient of E. coli SSB thus determined ranged from 70,000 to 170,000 nt2/s, which is at least 600 times higher than that determined from SSB diffusion on short ssDNA oligomers, and is within the range of values reported for protein diffusion on double-stranded DNA. Our work suggests that SSB can also migrate via a long-range intersegment transfer on long ssDNA. The force dependence of SSB movement on ssDNA further supports this interpretation.  相似文献   
2.
应用mRNA差异显示技术,对高耐SO2玉米自交系Q9进行了SO2胁迫下的基因差异表达分析.结果显示,30对引物组合进行的PCR扩增中,获得了13条差异表达的cDNA片段,其中3条诱导表达,2条增强表达,6条减量表达,2条抑制表达.序列分析和数据库比对表明,GenBank中只搜寻到5条cDNA序列,其中2条增强表达的cDNA片段D1和D5分别与氨基酸结合蛋白ABP、锌指结构转录因子蛋白DOF部分序列高度同源,其他诱导表达的3条cDNA(D3、D6、D11)功能未知,可能是新的cDNA片段.经半定量RT-PCR分析验证,D1和D5转录水平受SO2胁迫表达显著增强,推测D1和D5可能参与了玉米对SO2胁迫的抗性反应.  相似文献   
3.
对健康的日本沼虾(1.40±0.12)g分别肌肉注射0.6×10~6、2×10~6、4×10~6、6×10~6 CFU/mL的停乳链球菌茵液10μL、注射后3 h、6 h、9 h、12h、24h、5d、10d分别采集肝胰腺、肌肉和鳃组织,测定其中的总超氧化物歧化酶(T-SOD)活性,研究停乳链球菌时日本沼虾T-SOD活性的影响,研究结果显示,肝胰腺中T-SOD活性于注射5d后达到最大值(P0.05),10d后0.6×10~6、2×10~6和4×10~6 CFU/mL,组T-SOD活性下降并接近对照组水平,而最高浓度组(6×10~6 CFU/mL)T-SOD酶活性仅有对照组的46%。肌肉和鳃中T-SOD活性均在注射6 h后达到最大值,注射10 d后,4×10~6和6×10~6 CFU/mL组的肌肉和6×10~6 CFU/mL组的鳃组织中T-SOD活性均仅有对照组的50%左右。另外,肌肉组织中T-SOD的峰值远高于鳃和肝胰腺组织中的峰值。上述结果表明,停乳链球菌不仅影响日本沼虾机体T-SOD酶的活性。而且肌肉、鳃和肝胰腺组织中酶的活性应答时间不同,且组织内酶活性的应答与菌的感染方式有关,另外,注射高浓度停乳链球菌长时间后会降低其组织中T-SOD酶活性。  相似文献   
4.
本文以鼻咽癌细胞株CNE2为放射敏感性的研究对象,经不同剂量X射线照射及不同时间培养后分别提取总蛋白,用共聚焦显微拉曼光谱仪检测其拉曼光谱。统计分析表明:被测样品的拉曼光谱中观察到一些可以归属于蛋白质物质的较为明显的基团频率振动峰;不同剂量的X射线照射后,总蛋白质的平均拉曼光谱与对照组谱形基本一致,但与对照组间的光谱存在着对应峰信号强度的不同。实验提示:照射后谱峰强度的增大或减小,提示着相关物质含量有所改变。分析照射后癌细胞总蛋白拉曼光谱的变化情况,结合数学统计方法,以探寻放射敏感性的特征拉曼标志,可以作为研究肿瘤放射敏感性的手段之一。  相似文献   
5.
Length-frequency data collected from fish landings in the Kenya waters of Lake Victoria were used to estimate the growth parameters, total mortality rate and growth performance index in Oreochromis niloticus. The asymptotic length, (L ) and the ratio of the total mortality rate (Z) to the growth constant (K), were estimated to be 64.6 cm and 3.219 respectively. K was 0.254 y-1, Z was 0.818 y-1 and the growth performance index θ′ = Log10 K + 2 log10 L∞ = 3.025, which is rather high as compared to other tilapia populations in natural waters.  相似文献   
6.
Sterol O-acyltransferase 2 (SOAT2), also known as ACAT2, is the major cholesterol esterifying enzyme in the liver and small intestine (SI). Esterified cholesterol (EC) carried in certain classes of plasma lipoproteins is hydrolyzed by lysosomal acid lipase (LAL) when they are cleared from the circulation. Loss-of-function mutations in LIPA, the gene that encodes LAL, result in Wolman disease (WD) or cholesteryl ester storage disease (CESD). Hepatomegaly and a massive increase in tissue EC levels are hallmark features of both disorders. While these conditions can be corrected with enzyme replacement therapy, the question arose as to what effect the loss of SOAT2 function might have on tissue EC sequestration in LAL-deficient mice. When weaned at 21 days, Lal/:Soat2+/+ mice had a whole liver cholesterol content (mg/organ) of 24.7 mg vs 1.9 mg in Lal+/+:Soat2+/+ littermates, with almost all the excess sterol being esterified. Over the next 31 days, liver cholesterol content in the Lal/:Soat2+/+ mice increased to 145 ± 2 mg but to only 29 ± 2 mg in their Lal/:Soat2/ littermates. The level of EC accumulation in the SI of the Lal/:Soat2/ mice was also much less than in their Lal/:Soat2+/+ littermates. In addition, there was a >70% reduction in plasma transaminase activities in the Lal/:Soat2/ mice. These studies illustrate how the severity of disease in a mouse model for CESD can be substantially ameliorated by elimination of SOAT2 function.  相似文献   
7.
湖南会同不同年龄杉木人工林土壤磷素特征   总被引:14,自引:4,他引:10  
对湖南会同不同年龄(7年生、17年生、25年生)杉木人工林土壤全磷、有效磷、无机磷组分和有机磷进行了研究,结果表明:3种不同林龄杉木林土壤全磷和有效磷的含量分别在317.06—398.56 mg/kg和0.82—1.38 mg/kg之间,土壤全磷和速效磷含量均属低水平;杉木林土壤全磷含量从7年生幼龄林到25a近熟林出现先升高后降低的规律,并且17年生和25年生林分比7年生林分分别增加了19.68%、15.75%,土壤有效磷含量17年生和25年生林分比7年生林分提高了45.55%左右;土壤磷素活化系数均小于2.0%,这表明本研究区土壤全磷向速效磷转化较难,土壤中磷素的有效性较低,但该值随着林分年龄的增加而出现增大的现象;无机磷含量分别为:7年生169.50 mg/kg、17年生182.03 mg/kg、25年生175.94 mg/kg,从幼龄林到中龄林增高,中龄林以后降低;土壤中无机磷组分以O-P含量最高,其次是Fe-P,Ca-P,Al-P最少;杉木不同生长发育阶段对无机磷形态的吸收是有选择性的,幼龄林到中龄林阶段林木以吸收Al-P为主,近熟林阶段林木以吸收Fe-P和Ca-P为主;有机磷含量在全磷所占比例随林龄的变化来看,杉木生长过程中有部分的有机磷矿化为无机磷。土壤不同形态磷的相关性分析结果显示,土壤有效磷与有机磷相关系数为0.667,呈极显著相关性,是研究区杉木人工林土壤有效磷的主要来源。  相似文献   
8.
In the present study 36 police officers were exposed to a psychological stressor (IQ quiz) and to cold pressor stress while several cardiovascular variables were monitored. Impedance cardiography was used to provide measures of heart rate, stroke volume, cardiac output, myocardial contractility, and total peripheral resistance. In addition, measures of systolic and diastolic blood pressure and peripheral skin temperature were obtained. A multivariate analysis of variance (MANOVA) indicated that significant increases in diastolic and systolic blood pressure during the cold pressor test were mediated by large increases in total peripheral resistance, whereas blood pressure elevation during the IQ quiz were accompanied by significant increases in heart rate and, to a lesser extent, cardiac output. Peripheral skin temperature decreased in response to each stressor. Additional analysis indicated a degree of stimulus specificity for several variables. For example, diastolic blood pressure showed greater increases to cold pressor than quiz, whereas systolic blood pressure increased more with the psychological than the physical stressor. Directional fractionation occurred for both myocardial contractility and cardiac output.  相似文献   
9.
From dilution series in defined mineral medium, a marine iregular coccoid methanogenic bacterium (strain MTP4) was isolated that was able to grow on methanethiol as sole source of energy. The strain also grew on dimethylsulfide, mono-, di-, and trimethylamine, methanol and acetate. On formate the organism produced methane without significant growth. Optimal growth on MT, with doubling times of about 20 h, occurred at 30°C in marine medium. The isolate required p-aminobenzoate and a further not identified vitamin. Strain MTP4 had a high tolerance to hydrogen sulfide but was very sensitive to mechanical forces or addition of detergents such as Triton X-100 or sodium dodecylsulfate. Methanethiol was fermented by strain MTP4 according to the following equation:
  相似文献   
10.
Despite advances in restoration of degraded lands around the world, native plants are still underutilized. Selection of appropriate plant materials is a critical factor in determining plant establishment and persistence. To better inform decision‐making, we examined cold‐hardiness dynamics, flowering phenology, and survival among five geographically distinct sulfur‐flower buckwheat (Polygonaceae: Eriogonum umbellatum Torr.) populations in a common garden. LT50 (a measure of freezing injury) was determined every 6 weeks across a complete year; one population was also evaluated at the source. Cold‐hardiness dynamics were similar across populations, with annual fluctuations in mean LT50 exceeding 40°C. Rate of deacclimation (i.e. loss of cold tolerance) in spring varied across populations and was not related to the elevation from which a population came. Plants were less cold hardy in October 2014 compared to October 2013, likely reflecting a response to colder local conditions in 2013. Although the range of LT50 was similar for a single comparison of common garden versus wild‐grown plants, wild‐grown plants acclimated and deacclimated earlier than common garden‐grown plants. Plants derived from a low‐elevation population showed delayed flowering phenology, while high‐elevation populations showed earlier flowering phenology, with one high‐elevation population having the lowest survival rate in the common garden. These results suggest that while considerable plasticity in seasonal cold‐hardiness dynamics occur, population variability in deacclimation and flowering phenology have implications for selection and movement of sulfur‐flower buckwheat for ecological restoration.  相似文献   
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