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1.
Adiponectin promotes human jaw bone marrow mesenchymal stem cell chemotaxis via CXCL1 and CXCL8 下载免费PDF全文
《Journal of cellular and molecular medicine》2017,21(7):1411-1419
Adiponectin (APN) is known to promote the osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (h‐JBMMSCs). However, the underlying mechanism has not been fully elucidated. Previously, we showed that APN could promote h‐JBMMSC osteogenesis via APPL1‐p38 by up‐regulating osteogenesis‐related genes. Here, we aimed to determine whether APN could promote h‐JBMMSC chemotaxis through CXCL1/CXCL8. The CCK‐8, wound healing and transwell assays were used to evaluate the proliferation, migration and chemotaxis of h‐JBMMSCs with or without APN treatment. Chemotaxis‐related genes were screened using RNA‐seq, and the results were validated using real‐time PCR and ELISA. We also performed Western blot using the AMPK inhibitor, WZ4003, and the p38 MAPK inhibitor, SB203580, to identify the signalling pathway involved. We found that APN could promote h‐JBMMSC chemotaxis in the co‐culture transwell system. CXCL1 and CXCL8 were screened and confirmed as the up‐regulated target genes. The APN‐induced CXCL1/8 up‐regulation to promote chemotaxis could be blocked by CXCR2 inhibitor SB225002. Western blot revealed that the phosphorylation of AMPK and p38 MAPK increased in a time‐dependent manner with APN treatment. Additionally, WZ4003 and SB203580 could suppress the APN‐induced overexpression of CXCL1 and CXCL8. The results of the transwell chemotaxis assay also supported the above results. Our data suggest that APN can promote h‐JBMMSC chemotaxis by up‐regulating CXCL1 and CXCL8. 相似文献
2.
Tests of associational defence provided by hairy plants for glabrous plants of Arabidopsis halleri subsp. gemmifera against insect herbivores 下载免费PDF全文
1. Trichome‐producing (hairy) and trichomeless (glabrous) plants of Arabidopsis halleri subsp. gemmifera were investigated to test whether plant resistance to herbivory depends on the plants' phenotypes and/or the phenotypes of neighbouring plants (associational effects). 2. A common garden experiment was conducted in which the relative frequency of hairy and glabrous plants was manipulated. Two species of leaf‐chewing insects (larvae of a white butterfly and a cabbage sawfly) were found less often on hairy plants than on glabrous plants. By contrast, the numbers of aphids and flea beetles did not differ significantly between hairy and glabrous plants. For none of these insects did abundance depend on the frequency of the two plant morphs. 3. A field survey was conducted in two natural populations of A. halleri. In the first population, a species of white butterfly was the dominant herbivore, and hairy plants incurred less leaf damage than glabrous plants across 2 years. By contrast, in the other population, where flea beetles were dominant, there were no consistent differences in leaf damage between the two types of plants. In neither of the two populations was any evidence found of associational effects. 4. This study did not provide any conclusive evidence of associational effects of anti‐herbivore resistance, but it was discovered that trichomes can confer resistance to certain herbivores. Given the results of previous work by the authors on associational effects against a flightless leaf beetle, such associational effects of the trichome dimorphism of A. halleri were herbivore‐specific. 相似文献
3.
Galina Belostotskaya Alexey Nevorotin Michael Galagudza 《Cell cycle (Georgetown, Tex.)》2015,14(19):3155-3162
Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit+, Sca+, and Isl1+ stem cells were revealed to be located inside the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The similar structures were also identified in the suspension of freshly isolated myocardial cells (ex vivo) of 20- and 40-day-old rats. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes. 相似文献
4.
5.
Takeo Horiguchi 《Phycological Research》1996,44(3):143-150
A new raphidophyte flagellate is described from the river mouth of the Daintree River, north-east Australia where there are extensive mangrove forests. The organism has two distinct phases: a club-shaped motile form, and a more or less spherical benthic non-motile form. The motile cell is metabolic and possesses 10–20 chloroplasts. The chloroplasts are imbricated, somewhat reminiscent of roofing tiles. A unique structure has been found at the posterior end of the cell. It is a tubular invagination, which consists of a single membrane supported by many small flattened vesicles. In addition, the structure is surrounded by many fibril-containing vesicles. The tubular invagination is different from both the contractile vacuoles and the pusules of dinoflagellates in both behavior and structure. Based on the possession of these unique features, it is concluded that the this mangrove flagellate should be described as a new species in a new genus and the name Haramonas dimorpha Horiguchi gen. et sp. nov. is proposed. 相似文献
6.
Ubiquity of Cysteine- and Metalloproteinase Activities in a Wide Range of Trypanosomatids 总被引:1,自引:0,他引:1
MARTA H. BRANQUINHA ALANE B. VERMELHO SAMUEL GOLDENBERG MYRNA C. BONALDO 《The Journal of eukaryotic microbiology》1996,43(2):131-135
We have analysed the proteinase profiles of 11 species from 7 different genera of trypanosomatids by in situ detection of enzyme activities on SDS-PAGE gels containing co-polymerized gelatin as substrate, and the use of specific proteinase inhibitors. Our survey indicates that while cysteine- and metalloproteinases are distributed ubiquitously among trypanosomatids, there are marked differences between the enzyme profiles from the monogenetic (Crithidia, Herpetomonas, Leptomonas) and digenetic (Trypanosoma, Endotrypanum, Phytomonas, Leishmania) species. The detected metalloproteinase activities, ranging in size from 50–100 kDa, partitioned into the detergent-phase after Triton X-114 extraction, while most of cysteine proteinases, of three distinct molecular mass ranges (30–50 kDa, 80–100 kDa and 116–205 kDa), partitioned into the aqueous phase. Thus, within this group of organisms, the metalloproteinase activities seem to be predominantly membrane-associated proteins. We also show that the plant parasites of the genus Phytomonas exhibit a distinctive cysteine proteinase profile that might be exploited further as a criterion for taxonomy of the genus. 相似文献
7.
8.
T. A. Jaarsma E. Lohmanns H. Hendriks T. W. J. Gadella T. M. Malingré 《Plant Systematics and Evolution》1990,169(1-2):31-39
InS. tuberosum subspp.tuberosum andnodosum, S. grandiflorum andS. ibericum the presence of the pyrrolizidine alkaloids lycopsamine, echimidine and symphytine could be demonstrated. The taxonS. tuberosum contains an unknown compound that seems to be specific for this taxon. This compound is not the pyrrolizidine alkaloid anadoline which has previously been reported for this species. It is possibly represented by a peak on GC/MS with a molecular ion peak at m/z 623 (as TMS derivative) and can be used as a chemotaxonomic marker for the speciesS. tuberosum. The pyrrolizidine alkaloid pattern of the two subspecies ofS. tuberosum reinforces the close relationship. Fresh material ofS. tuberosum contained the triterpene isobauerenol, but in herbarium material isobauerenol was lacking. InS. grandiflorum, neither fresh nor dried material contains isobauerenol. In herbarium material ofS. ibericum also no isobauerenol could be found. More extensive chemotaxonomical research is necessary to support the view thatS. abchasicum is more closely related toS. ibericum than toS. grandiflorum. 相似文献
9.
Growth form and distribution of introduced plants in their native and non-native ranges in Eastern Asia and North America 总被引:1,自引:1,他引:0
There is a growing interest in understanding the influence of plant traits on their ability to spread in non-native regions. Many studies addressing this issue have been based on relatively small areas or restricted taxonomic groups. Here, we analyse a large data base involving 1567 plant species introduced between Eastern Asia and North America or from elsewhere to both regions. We related the extent of species distributions in each region to growth form and the distinction between upland and wetland habitats. We identified significant relationships between geographical distribution and plant traits in both native and exotic ranges as well as regional differences in the relationships. Range size was larger for herbaceous graminoids and forbs, especially annuals compared to perennials, than for woody species, and range size also was larger for plants of wetland compared to upland habitats. Distributions were more extensive in North America than in Eastern Asia, although native plants from both regions had broader distributions than non-natives, with exotics from elsewhere intermediate. Growth form and environment explained more of the variance in distribution of plants in North America than in Eastern Asia. The influence of growth form and habitat on distribution suggests that these traits might be related to tolerance of ecological conditions. In addition, the smaller extents of species in non-native compared to native areas suggest roles for dispersal limitation and adaptation to region-specific ecological conditions in determining distribution. 相似文献
10.