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芹菜斑枯病菌生物学特性研究 总被引:2,自引:0,他引:2
以沈阳地区芹菜斑枯病菌的纯培养菌株为试材,通过对菌丝生长、孢子萌发的营养及环境条件的多因子试验,明确了芹菜斑枯病菌菌丝生长以在CDAMS—VI培养基上最好,其次是CSA、CCSA和PDA,菌丝生长及产孢对碳源、氮源种类有明显的选择性。菌丝生长的最适pH值为4~5,最适温度为21℃,致死温度为39℃(处理40min),光照明显抑制菌丝生长。病菌分生孢子在水和芹菜汁中不能萌发,在水琼脂上萌发最好;孢子萌发的适宜pH值为4.6~6.6,适温为17—25℃,致死温度为42℃(处理20min),RH100%最适宜孢子萌发。光照和变温处理可促进分生孢子器的形成,病菌在PDA上从孢子萌发到分生孢子器形成并释放出分生孢子需要16~18d。 相似文献
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Ana-Flora D. Vasconcelos Robert F. H. Dekker Aneli M. Barbosa Luzia Paccola-Meirelles 《Mycoscience》2001,42(6):543-548
Three species of botryosphaeriaceous fungi,Botryosphaeria sp. isolate MAMB-5,Botryosphaeria ribis andLasiodiplodia theobromae, were compared for the production of pycnidia and laccases. Laccases were produced both intra- and extra-cellularly when
the fungi were cultivated on basal medium in the presence and absence of veratryl alcohol, withBotryosphaeria sp. MAMB-5 showing the highest enzyme titres. Electrophoretic examination of intracellular marker proteins (esterases and
phosphatases) and laccases indicated that the three species were genetically distinctly different, although the laccase zymograms
for the three fungi showed similarity. The production of pycnidia occurred under continuous lighting at 28°C, but conditions
differed among the three fungal species. Production could be induced on artificial media (potato-dextrose and oat agar) under
stress-induced conditions where the mycelium was stimulated by physical abrasion, and in the case ofBotryosphaeria sp. isolate MAMB-5 on eucalypt woodchips. Evidence is presented that veratryl alcohol facillitated the secretion of intracellular-localised
laccases into the extracellular medium. 相似文献
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The effects of culture media, temperature and light on radial growth and pycnidium production of cowpea isolates (PB1, PB2, PB3) of Phoma bakeriana were investigated in vitro. There was no isolate effect on growth and pycnidium production but some evidence of a medium effect. Exceptionally high growth rate on two media corresponded with high pycnidium production; while low growth rates on other media were generally associated with high sporulation. There was a large temperature effect and an isolate effect on growth with a significant temperature by isolate interaction. Temperatures of 5°C, 10°C and 35°C inhibited pycnidium formation completely while the range 15–30°C had no effect on sporulation. Maximum growth and pycnidium production occurred at 25°C. Near-ultraviolet radiation seemed to have caused higher pycnidium counts than the other light sources. 相似文献
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