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1.
Abstract The parasitoid wasp Trichogramma dendrolimi Matsumura sets the number of progeny allocated to its insect egg hosts according to the duration of its initial transit walk across the host surface. Although cooling the wasp reduces its walking speed, reduced temperature does not affect progeny allocation. By locally heating and cooling the wasps, the initial transit can be thermally uncoupled from the subsequent oviposition. Using this technique we show that the timing of initial transit duration is temperature-dependent. These findings suggest that short interval timing by Trichogramma differs physiologically from the temperature compensated clocks that have been described for other insects. 相似文献
2.
《DNA Repair》2017
Human PrimPol is a recently discovered bifunctional enzyme that displays DNA template-directed primase and polymerase activities. PrimPol has been implicated in nuclear and mitochondrial DNA replication fork progression and restart as well as DNA lesion bypass. Published evidence suggests that PrimPol is a Mn2+-dependent enzyme as it shows significantly improved primase and polymerase activities when binding Mn2+, rather than Mg2+, as a divalent metal ion cofactor. Consistently, our fluorescence anisotropy assays determined that PrimPol binds to a primer/template DNA substrate with affinities of 29 and 979 nM in the presence of Mn2+ and Mg2+, respectively. Our pre-steady-state kinetic analysis revealed that PrimPol incorporates correct dNTPs with 100-fold higher efficiency with Mn2+ than with Mg2+. Notably, the substitution fidelity of PrimPol in the presence of Mn2+ was determined to be in the range of 3.4 × 10−2 to 3.8 × 10−1, indicating that PrimPol is an error-prone polymerase. Furthermore, we kinetically determined the sugar selectivity of PrimPol to be 57–1800 with Mn2+ and 150–4500 with Mg2+, and found that PrimPol was able to incorporate the triphosphates of two anticancer drugs (cytarabine and gemcitabine), but not two antiviral drugs (emtricitabine and lamivudine). 相似文献
3.
Robert A. Krebs 《Journal of Insect Behavior》1991,4(2):221-233
Variation in copulation duration of Drosophila mojavensisstrains was influenced by both sexes. Males maintained predominant control, as copulation duration of pairs from different strains was more similar to that of the strain from which the male was derived, but female origin also contributed significantly to the duration of copulation. Variation among strains was controlled by genes acting additively in both sexes. The size of both males and females also affected copulation duration. Small males copulated longer on average than large males, while males paired with large females copulated longer than those paired with small females. The importance of copulation duration to fitness was tested by correlation analyses with male size, female size, female remating latency, and number of eggs laid prior to female remating. Longer copulations stimulated earlier oviposition, possibly by increasing accessory gland secretions that are passed by males during copulation. 相似文献
4.
David O. Simelane 《Biocontrol Science and Technology》2006,16(9):971-979
Compatibility between the root-feeding agent Longitarsus bethae Savini & Escalona and the varieties of the target weed Lantana camara L. could influence the chances of successful establishment and effectiveness of this agent as a biocontrol agent of lantana in South Africa. Laboratory experiments were conducted to determine the influence of major South African lantana varieties on feeding, colonization, oviposition preference and larval survival of L. bethae. Feeding preference and the number of adults per plant varied among the varieties. The most damaged and colonized varieties were 150 Orange, 009 Light Pink and 113 Dark Pink. Variety 018 Dark Pink was the least damaged, while 021 Total Pink and 029 White Pink were the least colonized. Females laid the highest number of eggs on variety 150 Orange. The lowest number of eggs was recorded on Australian Richmond Pink and the closely related species Lippia wilmsii. Larval survival differed significantly among the varieties. The highest survival was recorded on 150 Orange. Duration of development also varied significantly among the varieties, with the slowest rate of development recorded on variety 029 Light Pink and the quickest on variety 015 White Yellow. Although variations in female body size were less significant among varieties, males emerging from variety 150 Orange were slightly smaller than those emerged from other varieties. There was a significant tendency for females to select varieties that enhanced the survival of their offspring, which met the predictions of the preference-performance hypothesis. The data suggest that the chances of establishment and successful control of the weed are expected to be much greater on those varieties that were preferred for oviposition and provided better larval performance. 相似文献
5.
Behavioral and reproductive effects of ultrasound on the Indian meal moth, Plodia interpunctella 总被引:1,自引:0,他引:1
Fangneng Huang & Bhadriraju Subramanyam 《Entomologia Experimentalis et Applicata》2004,113(3):157-164
The daily activity patterns of adult movement, female calling, and mating of the Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae), were examined both in the absence and presence of ultrasound. Moths were exposed to ultrasound from a commercial ultrasonic device (Cix 0600) that produces constant sound patterns, and from a device developed at Kansas State University (KSU device) that produces random sound patterns. Daily activity patterns of adult movement, female calling, and mating followed a similar trend in the absence or presence of ultrasound. Female calling and mating, both in the absence and presence of ultrasound, primarily occurred during scotophase (21.00–07.00 hours). Ultrasound from the two devices significantly reduced the frequency of female calling and mating relative to unexposed moths. Consequently, the number of spermatophores transferred by males to females and egg production were lower in females exposed to ultrasound compared with unexposed females. In the absence of ultrasound, female P. interpunctella mated 2.9 times, resulting in 2.8 spermatophores/female. In the presence of ultrasound from the Cix 0600 device, a female mated 2.1 times and had 1.7 spermatophores. Corresponding values for the KSU device were 1.9 and 1.4, respectively. In the absence of ultrasound, 78% of the matings lasted 30–90 min, whereas in the presence of ultrasound 45–58% of the matings lasted either less than 30 min or more than 90 min. Moths exposed to ultrasound laid 96–130 eggs female?1 compared with 229 eggs female?1 for unexposed moths. Ultrasound did not affect the pre‐oviposition period and adult longevity of P. interpunctella. 相似文献
6.
7.
1. As for some other spring‐feeding moths, adult flight of Epirrita autumnata (Lepidoptera: Geometridae) occurs in late autumn. Late‐season flight is a result of a prolonged pupal period. Potential evolutionary explanations for this phenological pattern are evaluated. 2. In a laboratory rearing, there was a weak correlation between pupation date and the time of adult emergence. A substantial genetic difference in pupal period was found between two geographic populations. Adaptive evolution of eclosion time can thus be expected. 3. Metabolic costs of a prolonged pupal period were found to be moderate but still of some ecological significance. Pupal mortality is likely to form the main cost of the prolonged pupal period. 4. Mortality rates of adults, exposed in the field, showed a declining temporal trend from late summer to normal eclosion time in autumn. Lower predation pressure on adults may constitute the decisive selective advantage of late‐season flight. It is suggested that ants, not birds, were the main predators responsible for the temporal trend. 5. Egg mortality was estimated to be low; it is thus unlikely that the late adult period is selected for to reduce the time during which eggs are exposed to predators. 6. In a laboratory experiment, oviposition success was maximal at the time of actual flight peak of E. autumnata, however penalties resulting from sub‐optimal timing of oviposition remained limited. 相似文献
8.
The intrinsic activity of the C‐terminal catalytic (C) domain of cyclic guanosine monophosphate (cGMP)‐dependent protein kinases (PKG) is inhibited by interactions with the N‐terminal regulatory (R) domain. Selective binding of cGMP to cyclic nucleotide binding (CNB) domains within the R‐domain disrupts the inhibitory R–C interaction, leading to the release and activation of the C‐domain. Affinity measurements of mammalian and plasmodium PKG CNB domains reveal different degrees of cyclic nucleotide affinity and selectivity; the CNB domains adjacent to the C‐domain are more cGMP selective and therefore critical for cGMP‐dependent activation. Crystal structures of isolated CNB domains in the presence and absence of cyclic nucleotides reveal isozyme‐specific contacts that explain cyclic nucleotide selectivity and conformational changes that accompany CNB. Crystal structures of tandem CNB domains identify two types of CNB‐mediated dimeric contacts that indicate cGMP‐driven reorganization of domain–domain interfaces that include large conformational changes. Here, we review the available structural and functional information of PKG CNB domains that further advance our understanding of cGMP mediated regulation and activation of PKG isozymes. 相似文献
9.
ALEX CÓRDOBA‐AGUILAR DANIELA RUIZ‐SILVA ROBERTO MUNGUÍA‐STEYER HUMBERTO LANZ‐MENDOZA 《Physiological Entomology》2011,36(4):335-342
Recent studies of female insects indicate that reproductive activities, such as mating and oviposition, can impair immune ability. Using the two tropical damselfly species Argia anceps Garrison and Hetaerina americana (Fabricius), egg production and phenoloxidase (PO) activity, a key enzyme in insect immunity, are measured in mating, ovipositing and perching females in December and March. Perching females of both species have fewer eggs compared with mating and ovipositing females, which suggests that perching females are not engaged in reproduction. There is seasonal variation in egg number for the three categories in H. americana but not in A. anceps, which can be interpreted in terms of adaptive changes in egg production depending on female–male interactions in the former species but not in the latter species. There is no difference in PO activity among mating, ovipositing or perching females within either species, although measurements in December and March indicate distinct seasonal changes. Juvenile Hormone (JH) is known to reduce the effectiveness of the immune system by favouring the use of resources for reproduction. A possible role for JH is examined in H. americana, using the JH analogue methoprene to manipulate hormone activity, revealing that PO activity is reduced in methoprene‐treated H. americana females. Thus, although the results of the present study are indicative of possible hormone‐driven changes in PO, there is not necessarily a down‐regulation of immune function (as determined by PO activity) during mating or oviposition. The results complement some recent studies countering the idea that reproductive activities reduce the immune ability in insects. 相似文献
10.
Aggregative oviposition behaviour of Helicoverpa spp. (Lepidoptera: Noctuidae) in contaminated chickpea crops 总被引:1,自引:0,他引:1
Abstract Aggregative egg distribution by Helicoverpa spp. within experimental field plots of chickpea containing various contaminant plant species was documented at different stages of plant growth. More eggs were laid on contaminant faba and wheat plants than on the surrounding chickpea. The level of egg aggregation on faba and wheat plants increased with general crop growth, becoming evident before a significant disparity in growth rates between these species and the surrounding chickpea became apparent. In chickpea plots contaminated with canola, aggregative oviposition activity on the contaminant plants was observed in the early seedling stage whilst a clear height differential between the two species was evident. Total Helicoverpa egg density on contaminant plants was not correlated with height above chickpea for any of the contaminant species. It is proposed that the aggregative oviposition behaviour of Helicoverpa spp. in contaminated chickpea results from differential apparency of the contaminant plants to ovipositing moths. Differential apparency may result from several factors including growth rate and morphological differences between chickpea and the contaminants. Physiological changes in chickpea, including growth stage-related changes in acid production by the foliage, may also contribute to the greater apparency of contaminant plants in chickpea plots. The implications of differential host plant apparency for insect pest management are discussed. 相似文献