非小细胞肺癌术后并发乳糜胸对患者生活质量影响的研究

目的:探讨非小细胞肺癌术后并发乳糜胸对患者生活质量的影响。方法:采用生活质量测定量表(QLQ-C30)回顾性分析第四军医大学唐都医院胸外科自2013年至2016年中收治的1015例肺癌手术患者的生活质量,发生乳糜胸组记为A组,未发生乳糜胸组记为B组。对比术前和术后1、3、6和12个月的生活质量有无统计学差异。结果:(1)术后1月时,除了社会功能、便秘、腹泻以外,两组生活质量指标评分均显著低于术前,且B组均显著低于A组,有统计学差异(表3,P0.05)。在手术后3月及以后逐渐恢复,至12月时,各组指标与术前基本相同(表3,P0.05);(2)两组术后生活质量相比较,术后1、3月,除社会功能、便秘、腹泻以外,其余生活质量功能指标B组均显著优于A组,有统计学差异(表3,P0.05)。在手术后6月及以后,B组所有指标与A组无统计学差异(表3,P0.05)。结论:肺癌根治术后发生乳糜胸患者生活质量显著低于未发生乳糜胸患者,因此应合理选择手术方式,注意术中操作,降低乳糜胸发生率,提高肺癌患者术后的生活质量。     

Evaluating the role of livelihood assets in suitable livelihood strategies: Protocol for anti-poverty policy in the Eastern Tibetan Plateau,China

Although numerous studies have been conducted on the relationship between livelihood assets and strategies, only a few quantitative studies exist on the topic for anti-poverty policies with regard to the Tibetan Plateau. This study investigated 357 households in the upper reaches of the Dadu River watershed in the Eastern Tibetan Plateau, China, using the participatory rural appraisal method. Then, applying a multinomial logistic regression model, we quantitatively analyzed the relationship between livelihood assets and livelihood strategies, the findings of which have implications for local agricultural policy interventions. The preliminary results indicate that of the four classifications of livelihood strategies used in this study, the main livelihood strategy is that of the non-farming-dependent household type (i.e., Type III in our study) that combines agricultural and non-farm activities or practices self-employment for wages. There are significant differences in the livelihood assets owned by households with different livelihood strategies. Human, natural, and financial assets have significant influences on livelihood strategies, and the choice of livelihood strategy varies by livelihood assets. Further, with improvements in household labor capacity and cash income per capita, the livelihood strategies of non-agricultural and non-farming households may shift away from agriculture, while an increase in farmland cultivated per capita, cash income per capita, and household labor capacity may encourage households to adopt agriculture-dependent livelihood strategies. These findings contribute to research on livelihood and related development strategies and anti-poverty policies in not only the Eastern Tibetan Plateau but also other regions with similar livelihood strategies.     

The evolution of intermediate castration virulence and ant coexistence in a spatially structured environment

Theory suggests that spatial structuring should select for intermediate levels of virulence in parasites, but empirical tests are rare and have never been conducted with castration (sterilizing) parasites. To test this theory in a natural landscape, we construct a spatially explicit model of the symbiosis between the ant-plant Cordia nodosa and its two, protecting ant symbionts, Allomerus and Azteca . Allomerus is also a castration parasite, preventing fruiting to increase colony fecundity. Limiting the dispersal of Allomerus and host plant selects for intermediate castration virulence. Increasing the frequency of the mutualist, Azteca , selects for higher castration virulence in Allomerus , because seeds from Azteca -inhabited plants are a public good that Allomerus exploits. These results are consistent with field observations and, to our knowledge, provide the first empirical evidence supporting the hypothesis that spatial structure can reduce castration virulence and the first such evidence in a natural landscape for either mortality or castration virulence.     

无功能垂体瘤的术后评估

垂体瘤是常见的颅内良性肿瘤,患病率高,预后较好。在我国,其发病率仅次于胶质瘤和脑膜瘤。无功能垂体瘤约占垂体瘤22.5%,手术是其首选的治疗方式,以解除肿瘤的占位效应,减少对正常垂体组织的压迫。然而手术本身可能引起垂体功能减退,术后应对患者垂体功能的监测并予以处理。同时,由于手术及患者个体身体、心理原因,患者术后的生活质量也越来越受到重视。本文综述了无功能垂体瘤患者术后各内分泌轴功能的临床检测方法,以帮助临床医生对术后患者的垂体功能进行评估并重建;同时对术后患者生活质量的评估加以讨论,帮助指导患者恢复生理及心理健康。     

Identification of cardiac stem cells within mature cardiac myocytes

Cardiac stem cells are described in a number of mammalian species including humans. Cardiac stem cell clusters consisting of both lineage-negative and partially committed cells are generally identified between contracting cardiac myocytes. In the present study, c-kit⁺, Sca⁺, and Isl1⁺ stem cells were revealed to be located inside the sarcoplasm of cardiac myocytes in myocardial cell cultures derived from newborn, 20-, and 40-day-old rats. Intracellularly localized cardiac stem cells had a coating or capsule with a few pores that opened into the host cell sarcoplasm. The similar structures were also identified in the suspension of freshly isolated myocardial cells (ex vivo) of 20- and 40-day-old rats. The results from this study provide direct evidence for the replicative division of encapsulated stem cells, followed by their partial cardiomyogenic differentiation. The latter is substantiated by the release of multiple transient amplifying cells following the capsule rupture. In conclusion, functional cardiac stem cells can reside not only exterior to but also within cardiomyocytes.     

一种新型免疫层析技术在临床乳腺癌Her-2蛋白定量检测中的应用

建立一种靶点蛋白质快速定量检测方法。在原有侧向流动免疫层析技术的基础上,通过优化层析材料和纳米微球的均一性、改进检测区的检测方法,经逐点扫描技术,建立标准浓度曲线,以达到对临床靶点蛋白质的定量检测。以乳腺癌组织中的Her2表达为例,通过对已知浓度样品的检测,验证本技术方法的准确度大于96%。另外,以蛋白质免疫印迹作为组织中特定蛋白质检测金标准,分析临床肿瘤组织中Her2蛋白的含量,其准确率也达到95.5%,而免疫组织化学方法检测准确率仅为69.58%。新型免疫层析法检测结果与靶向治疗患者的愈后密切相关(P<0.01)。改进后的新型免疫层析方法能够准确地对临床靶点蛋白质进行定量检测,而且结合侧向流动技术的简单、快速和易用性,这种新型检测方法可以广泛应用于临床组织标本、血液标本和体液标本中靶点蛋白质的临场定量检测,在一定程度上可以替代免疫组化技术。     

The value of harmful algal bloom predictions to the nearshore commercial shellfish fishery in the Gulf of Maine

In this study, we develop a framework for measuring the value of harmful algal bloom (HAB) predictions. The framework captures the effects of both private and public responses to HABs. Using data from the New England nearshore commercial shellfish fishery and impact estimates for a large-scale HAB event in 2005, we illustrate how the potential value of HAB forecasts may be estimated. The results of our study suggest that the long-term value of a HAB prediction and tracking system for the Gulf of Maine is sensitive to the frequency of HAB events, the accuracy of predictions, the choice of HAB impact measures, and the effectiveness of public and private responses.     

A general framework to describe the alteration of natural tree species composition as an indicator of forest naturalness

The alteration of natural tree species composition is defined as the deviation of the current tree species composition from that of the natural state. It can be used as a measure of human influence on forest vegetation, and thus as an indicator of the naturalness of forest vegetation. The aim of the study was to develop a standard procedure for estimating the alteration of natural tree species composition, to explain factors driving alteration and to examine its significance for susceptibility of forest stands to natural disturbances. The alteration of natural tree species composition was estimated for the Dinaric region (5556 km², Slovenia) by the Robič Index of Dissimilarity (RID), ranging from 0 (completely natural) to 100 (completely altered). The index was calculated on the compartment level (24 ha each on average) with data on current and potential natural forest vegetation. The influence of human activities on tree species alteration was examined by using topographic and accessibility variables. The susceptibility of forest stands to natural disturbances was analysed with data on sanitary felling. In the study area, the natural tree species composition of forest stands is moderately preserved; the average value of RID was 50.05, ranging from 1.76 to 100, and the coefficient of variation was 0.49. The alteration of the natural tree species composition of forest stands is primarily the result of forest management and past land use, conditioned either by topography or accessibility of forests. The degree of alteration of tree species composition decreased along the gradients of rockiness, inclination and elevation. A greater degree of alteration appeared on the slopes of intermediate and south facing aspects than on north facing slopes, and in areas that were closer to the forest edge. A higher level of alteration significantly increases the susceptibility of forest stands to natural disturbances. The procedure represents a novel approach in modelling the alteration (naturalness) of tree species composition of forest vegetation. It is applicable at different spatial scales and fosters an understanding of the patterns of tree species composition under the influence of human activity across forest landscapes.