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1.
B. I. Sukhorukov S. A. Tikhonenko E. A. Saburova A. V. Dubrovskii Yu. N. Dybovskaya L. I. Shabarchina 《Biophysics》2007,52(6):575-581
Encapsulation of enzymes (lactate dehydrogenase and urease) in polyelectrolyte shells was assessed with a view to designing enzymic microdiagnostics for low-molecular compounds in native biological fluids. Polyelectrolyte microcapsules were prepared with two polyanions [poly(styrenesulfonate) PSS and dextran sulfate DS] and two polycations [poly(allylamine) PAA and poly(diallyldimethylammonium) PDADMA]; calcium carbonate microspherulites with embedded enzymes served as “cores.” It was demonstrated that the main problem in making such a biosensor is to select a pair of oppositely charged polyelectrolytes that would be optimal for enzyme functioning. The best pairs were PAA/DS and PAA/PSS for lactate dehydrogenase, and PSS/PAA and PSS/PDADMA for urease. We designed and prepared enzyme-containing microcapsules differing in polyelectrolyte composition and number of layers, and investigated their properties. 相似文献
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Differential role of microenvironment in microencapsulation for improved cell tolerance to stress 总被引:6,自引:0,他引:6
Sun ZJ Lv GJ Li SY Yu WT Wang W Xie YB Ma X 《Applied microbiology and biotechnology》2007,75(6):1419-1427
The effect of the microenvironment in alginate–chitosan–alginate (ACA) microcapsules with liquid core (LCM) and solid core
(SCM) on the physiology and stress tolerance of Sacchromyces cerevisiae was studied. The suspended cells were used as control. Cells cultured in liquid core microcapsules showed a nearly twofold
increase in the intracellular glycerol content, trehalose content, and the superoxide dismutase (SOD) activity, which are
stress tolerance substances, while SCM did not cause the significant physiological variation. In accordance with the physiological
modification after being challenged with osmotic stress (NaCl), oxidative stress (H2O2), ethanol stress, and heat shock stress, the cell survival in LCM was increased. However, SCM can only protect the cells
from damaging under ethanol stress. Cells released from LCM were more resistant to hyperosmotic stress, oxidative stress,
and heat shock stress than cells liberated from SCM. Based on reasonable analysis, a method was established to estimate the
effect of microenvironment of LCM and SCM on the protection of cells against stress factors. It was found that the resistance
of LCM to hyperosmotic stress, oxidative stress, and heat shock stress mainly depend on the domestication effect of LCM’s
microenvironment. The physical barrier of LCM constituted by alginate–chitosan membrane and liquid alginate matrix separated
the cells from the damage of oxidative stress and ethanol stress. The significant tolerance against ethanol stress of SCM
attributed to the physical barrier consists of solid alginate–calcium matrix and alginate–chitosan membrane. 相似文献
4.
T. N. Borodina L. D. Rumsh S. M. Kunizhev G. B. Sukhorukov G. N. Vorozhtsov B. M. Feldman A. V. Rusanova T. V. Vasil’eva S. M. Strukova E. A. Markvicheva 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2008,2(2):176-182
The microcapsules with entrapped herbal water-soluble extracts of plantain Plantago major and calendula Calendula officinalis L. (PCE) were prepared by layer-by-layer (LbL)-adsorption of carrageenan and oligochitosan onto CaCO3 microparticles with their subsequent dissolving after the treatment of EDTA. Entrapment of PCE was performed by using adsorption and co-precipitation techniques. The co-precipitation provided better entrapment of PCE into the carbonate matrix compared to adsorption. In vitro release kinetics (AGJ) was studied using artificial gastric juice. Using the model of acetate ulcer in rats it has been demonstrated that PCE released from the microcapsules accelerates gastric tissue repair. 相似文献
5.
Biologic effect and immunoisolating behavior of BMP-2 gene-transfected bone marrow-derived mesenchymal stem cells in APA microcapsules 总被引:2,自引:0,他引:2
Ding HF Liu R Li BG Lou JR Dai KR Tang TT 《Biochemical and biophysical research communications》2007,362(4):923-927
We investigated the encapsulation of BMP-2 gene-modified mesenchymal stem cells (MSCs) in alginate-poly-L-lysine (APA) microcapsules for the persistent delivery of bone morphogenic protein-2 (BMP-2) to induce bone formation. An electrostatic droplet generator was employed to produce APA microcapsules containing encapsulated beta-gal or BMP-2 gene-transfected bone marrow-derived MSCs. We found that X-gal staining was still positive 28 days after encapsulation. Encapsulated BMP-2 gene-transfected cells were capable of constitutive delivery of BMP-2 proteins for at least 30 days. The encapsulated BMP-2 gene-transfected MSCs or the encapsulated non-gene transfer MSCs (control group) were cocultured with the undifferentiated MSCs. The gene products from the encapsulated BMP-2 cells could induce the undifferentiated MSCs to become osteoblasts that had higher alkaline phosphatase (ALP) activity than those in the control group (p<0.05). The APA microcapsules could inhibit the permeation of fluorescein isothiocyanate-conjuncted immunoglobulin G. Mixed lymphocyte reaction also indicates that the APA microcapsules could prevent the encapsulated BMP-2 gene-transfected MSCs from initiating the cellular immune response. These results demonstrated that the nonautologous BMP-2 gene-transfected stem cells are of potential utility for enhancement of bone repair and bone regeneration in vivo. 相似文献
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The purpose of this study was to examine the suitability of polystyrene-coated (PS-coated) microcapsules of drug-resin complex
for achieving prolonged release of diltiazem-HCl, a highly water-soluble drug, in simulated gastric and intestinal fluid.
The drug was bound to Indion 254, a cation-exchange resin, and the resulting resinate was microencapsulated with PS using
an oil-in-water emulsion-solvent evaporation method. The effect of various formulation parameters on the characteristics of
the microcapsules was studied. Mean diameter and encapsulation efficiency of the microcapsules rose with an increase in the
concentration of emulsion stabilizer and the coat/core ratio, while the same characteristics tended to decrease with an increase
in the volume of the organic disperse phase. The desorption of drug from the uncoated resinate was quite rapid and independent
of the pH of the dissolution media. On the other hand, the drug release from the microcapsules was prolonged for different
periods of time depending on the formulation parameters and was also found to be independent of the pH of the dissolution
media. Both the encapsulation efficiency and the retardation of drug release were found to be dependent on the uniformity
of coating, which in turn was influenced by the formulation parameters. Kinetic studies revealed that the desorption of drug
from the resinate obeyed the typical particle diffusion process, whereas the drug release from the microencapsulated resinate
followed the diffusion-controlled model in accordance with the Higuchi equation. PS appeared to be a suitable polymer to provide
prolonged release of diltiazem independent of the pH of the dissolution media. 相似文献
7.
Electroantennogram measurements of atmospheric pheromone concentration after aerial and ground application of gypsy moth mating disruptants 总被引:1,自引:0,他引:1
K. W. Thorpe J. van der Pers D. S. Leonard P. Sellers V. C. Mastro R. E. Webb R. C. Reardon 《Journal of Applied Entomology》2007,131(2):146-152
Abstract: A portable electroantennogram (EAG) sensor was used to measure relative atmospheric pheromone concentration in forest plots treated with aerial and ground applications of gypsy moth, Lymantria dispar (L.) (Lep., Lymantriidae), mating-disruption formulations. Five treatments (Disrupt II flakes with sticker, Disrupt II flakes without sticker, Disrupt II flakes in a sticker slurry, microcapsules and hand-applied Luretape), all applied at 75 g active ingredient per hectare and an untreated control were evaluated. Gypsy moth male catch in pheromone-baited traps and fertilization of deployed females were suppressed in all treatments, and no females deployed in treated plots produced more than 5% fertile eggs. Relative pheromone concentrations were significantly higher in the two treatments in which flakes were aerially applied with sticker and in the microcapsule treatment. Pheromone concentration measurements in the flakes without sticker and hand-applied treatments were not significantly different from those in the control. Mating success was negatively correlated with relative pheromone concentration. The ability of the EAG to detect differences in pheromone concentration that are correlated with mating success suggests that this could be a useful method for predicting the effectiveness of mating-disruption treatments. 相似文献
8.
Polyelectrolyte-coated microcapsules and their potential applications to biotechnology 总被引:1,自引:0,他引:1
Etsuo Kokufuta 《Bioseparation》1998,7(4-5):241-252
Polyelectrolyte-coated microcapsules can be prepared by adsorption of polyions onto microcapsule surfaces in aqueous solutions
under appropriate pH and ionic conditions. The resulting polyelectrolyte-coated microcapsules provide a promising tool for
studying pH-induced configurational changes in polyions adsorbed onto hydrophobic membranes (capsule walls). An interesting
application of polyelectrolyte-coated microcapsules is the pH-sensitive on/off control of microencapsulated enzyme reactions
through alterations in the substrate permeability of the capsule wall by pH-conditioned configurational changes in the adsorbed
polyion layer. This paper presents an overview of pH-induced conformational changes of polyelectrolytes in solutions, preparation
of polyelectrolyte-coated microcapsules with an immobilized enzyme, and on/off control of the respective enzyme reactions
by pH adjustment.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
9.
Lu Xu Qiwen Shen Zhongqi Mao L. James Lee Ouliana Ziouzenkova 《Journal of visualized experiments : JoVE》2015,(100)
Cell encapsulation was developed to entrap viable cells within semi-permeable membranes. The engrafted encapsulated cells can exchange low molecular weight metabolites in tissues of the treated host to achieve long-term survival. The semipermeable membrane allows engrafted encapsulated cells to avoid rejection by the immune system. The encapsulation procedure was designed to enable a controlled release of bioactive compounds, such as insulin, other hormones, and cytokines. Here we describe a method for encapsulation of catabolic cells, which consume lipids for heat production and energy dissipation (thermogenesis) in the intra-abdominal adipose tissue of obese mice. Encapsulation of thermogenic catabolic cells may be potentially applicable to the prevention and treatment of obesity and type 2 diabetes. Another potential application of catabolic cells may include detoxification from alcohols or other toxic metabolites and environmental pollutants. 相似文献
10.
In this study an attempt was made to prepare mucoadhesive microcapsules of gliclazide using various mucoadhesive polymers
designed for oral controlled release. Gliclazide microcapsules were prepared using sodium alginate and mucoadhesive polymer
such as sodium carboxymethyl cellulose (sodium CMC), carbopol 934P or hydroxy propylmethyl cellulose (HPMC) by orifice-ionic
gelation method. The microcapsules were evaluated for surface morphology and particle shape by scanning electron microscope.
Microcapsules were also evaluated for their microencapsulation efficiency, in vitro wash-off mucoadhesion test, in vitro drug release and in vivo study. The microcapsules were discrete, spherical and free flowing. The microencapsulation efficiency was in the range of
65–80% and microcapsules exhibited good mucoadhesive property in the in vitro wash off test. The percentage of microcapsules adhering to tissue at pH 7.4 after 6 h varied from 12–32%, whereas the percentage
of microcapsules adhering to tissue at pH 1.2 after 6 h varied from 35–68%. The drug release was also found to be slow and
extended for more than 16 h. In vivo testing of the mucoadhesive microcapsules in diabetic albino rats demonstrated significant antidiabetic effect of gliclazide.
The hypoglycemic effect obtained by mucoadhesive microcapsules was for more than 16 h whereas gliclazide produced an antidiabetic
effect for only 10 h suggesting that mucoadhesive microcapsules are a valuable system for the long term delivery of gliclazide. 相似文献