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1.
Isabel Bandín Ysabel Santos Beatriz Magariños Juan L. Barja Alicia E. Toranzo 《FEMS microbiology letters》1992,94(1-2):105-110
The analysis of the membrane proteins and their antigenic properties in a group of 14 geographically diverse strains of Renibacterium salmoninarum revealed the existence of antigenic diversity within this species. Eleven isolates, including the type strain ATCC 33209, shared a similar protein profile with a major component of 57 kDa whereas three strains showed a common pattern with a major protein of 30 kDa. The quantitative agglutination tests and Western blotting assays seem to indicate the existence of serological heterogeneity, with two distinct groups being detected. 相似文献
2.
1. Insect galls are abnormal plant growths that develop in response to a stimulus provided by a galling insect. The nutrition hypothesis suggests that the concentrations of nutritive compounds in galls are changed to provide optimum nutrition for the larvae and adults of galling insects. 2. To test the nutrition hypothesis, we determined the concentrations of 20 free amino acids in galls and in galled and ungalled twigs of Quercus fabri during the larval and adult stages of Andricus mukaigawae using high-performance liquid chromatography with ultraviolet–visible detection. 3. At the larval stage, the concentrations of 12 out of 20 amino acids in A. mukaigawae galls were significantly higher than those in galled and ungalled twigs. Asparagine and tryptophan were the most abundant nonessential and essential amino acids, respectively, in A. mukaigawae galls. 4. At the adult stage, the concentrations of most amino acids, except proline, were significantly lower in A. mukaigawae galls than in galled and ungalled twigs. The A. mukaigawae adults may not manipulate amino acid levels because the adults do not feed on galls. The decrease of amino acid levels in adult galls may be viewed as a depletion. 5. The composition of free amino acids in A. mukaigawae galls was significantly different from the composition in galled and ungalled twigs in both the larval and adult stages. 6. Our results may support the nutrition hypothesis. We suggest that a high concentration of proline in A. mukaigawae galls may protect larvae and adults from plant defense responses. 相似文献
3.
Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers 总被引:1,自引:0,他引:1
M. L. Ali J. F. Rajewski P. S. Baenziger K. S. Gill K. M. Eskridge I. Dweikat 《Molecular breeding : new strategies in plant improvement》2008,21(4):497-509
Sweet sorghum (Sorghum bicolor L.) is a type of cultivated sorghums and has been recognized widely as potential alternative source of bio-fuel because of
its high fermentable sugar content in the stalk. A substantial variation of sugar content and related traits is known to exist
in US sweet sorghum. The objectives of the study were to assess the genetic diversity and relationship among the US sweet
sorghum cultivars and lines using SSR markers and to examine the genetic variability within sweet sorghum accessions for sugar
content. Sixty-eight sweet sorghum and four grain sorghum cultivars and lines were genotyped with 41 SSR markers that generated
132 alleles with an average of 3.22 alleles per locus. Polymorphism information content (PIC) value, a measure of gene diversity,
was 0.40 with a range of 0.03–0.87. The genetic similarity co-efficient was estimated based on the segregation of the 132
SSR alleles. Clustering analysis based on the genetic similarity (GS) grouped the 72 sorghum accessions into 10 distinct clusters.
Grouping based on clustering analysis was in good agreement with available pedigree and genetic background information. The
study has revealed the genetic relationship of cultivars with unknown parentage to those with known parentage. A number of
diverse pairs of sweet sorghum accessions were identified which were polymorphic at many SSR loci and significantly different
for sugar content as well. Information generated from this study can be used to select parents for hybrid development to maximize
the sugar content and total biomass, and development of segregating populations to map genes controlling sugar content in
sweet sorghum. 相似文献
4.
Evolution of antigenic diversity in the tick‐transmitted bacterium Borrelia afzelii: a role for host specialization? 下载免费PDF全文
L. Råberg Å. Hagström M. Andersson S. Bartkova K. Scherman M. Strandh B. Tschirren 《Journal of evolutionary biology》2017,30(5):1034-1041
Antigenic diversity in pathogenic microbes can be a result of at least three different processes: diversifying selection by acquired immunity, host–pathogen coevolution and/or host specialization. Here, we investigate whether host specialization drives diversity at ospC (which encodes an immunodominant surface protein) in the tick‐transmitted bacterium Borrelia afzelii. We determined prevalence and infection intensity of ospC strains in naturally infected wild mammals (rodents and shrews) by 454 amplicon sequencing in combination with qPCR. Neither prevalence nor infection intensity of specific ospC strains varied in a species‐specific manner (i.e. there were no significant ospC × host species interactions). Rankings of ospC prevalences were strongly positively correlated across host species. Rankings of ospC infection intensities were correlated more weakly, but only in one case significantly < 1. ospC prevalences in the studied mammals were similar to those in ticks sampled at the study site, indicating that we did not miss any mammal species that are important hosts for specific ospC strains. Based on this, we conclude that there is at best limited host specialization in B. afzelii and that other processes are likely the main drivers of ospC diversity. 相似文献
5.
Thermal tolerance and recovery behaviour of Thorectes lusitanicus (Coleoptera,Geotrupidae) 下载免费PDF全文
BELÉN GALLEGO JOSÉ R. VERDÚ LUIS M. CARRASCAL JORGE M. LOBO 《Ecological Entomology》2017,42(6):758-767
1. Population differences in physiological responses are examined in Thorectes lusitanicus, an endemic Iberian dung beetle species, by submitting individuals of different populations to the same experimental and acclimation conditions. 2. An infrared thermography protocol was used, consisting of three assays: start of activity, cold response, and heat response. Individuals of 12 populations were studied and the comparative explanatory capacities of several environmental factors in relation to the observed inter‐population differences were examined. 3. The heating rate from chill coma to the beginning of activity was the variable with the highest discrimination power among the studied populations, accounting for 94% of the observed variance. Regarding the heat response, only six of the 16 thermal variables reached significance (inter‐population differences accounted for 52–74% in these six thermal parameters). 4. From the three considered environmental factors (Mediterranean climate, land cover, and trophic characteristics) only land cover characteristics remain statistically significant, affecting the cold response of individuals. 5. Thorectes lusitanicus is a species characterised by a high diversity of thermotolerance and recovery traits across populations with a low degree of association with broad environmental factors. Finally, it is suggested that the apterous character of this species could be a determinant factor explaining the high diversity of ecophysiological traits related to thermal stress tolerance and the recovery time. 相似文献
6.
J. L. Petzold‐Maxwell B. D. Siegfried R. L. Hellmich C. A. Abel B. S. Coates T. A. Spencer R. J. Horikoshi A. J. Gassmann 《Journal of Applied Entomology》2017,141(1-2):67-79
Crops producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely planted to manage insect pests. Bt crops can provide an effective tool for pest management; however, the evolution of Bt resistance can diminish this benefit. The European corn borer, Ostrinia nubilalis Hübner, is a significant pest of maize and is widely managed with Bt maize in the Midwest of the United States. When Bt crops are grown in conjunction with non‐Bt refuges, fitness costs of Bt resistance can delay the evolution of resistance. Importantly, fitness costs often vary with ecological factors, including host‐plant genotype and diapause. In this study, we examined fitness costs associated with Cry1F resistance in O. nubilalis when insects were reared on three maize lines. Fitness costs were tested in two experiments. One experiment assessed the fitness costs when Cry1F‐resistant and Cry1F‐susceptible insects were reared on plants as larvae and experienced diapause. The second experiment tested resistant, susceptible and F1 heterozygotes that were reared on plants but did not experience diapause. Despite some evidence of greater adult longevity for Cry1F‐resistant insects, these insects produced fewer fertile eggs than Cry1F‐susceptible insects, and this occurred independent of diapause. Reduced fecundity was not detected among heterozygous individuals, which indicated that this fitness cost was recessive. Additionally, maize lines did not affect the magnitude of this fitness cost. The lower fitness of Cry1F‐resistant O. nubilalis may contribute to the maintenance of Cry1F susceptibility in field populations more than a decade after Cry1F maize was commercialized. 相似文献
7.
8.
E. Ciani P. Crepaldi L. Nicoloso E. Lasagna F. M. Sarti B. Moioli F. Napolitano A. Carta G. Usai M. D'Andrea D. Marletta R. Ciampolini V. Riggio M. Occidente D. Matassino D. Kompan P. Modesto N. Macciotta P. Ajmone‐Marsan F. Pilla 《Animal genetics》2014,45(2):256-266
Italy counts several sheep breeds, arisen over centuries as a consequence of ancient and recent genetic and demographic events. To finely reconstruct genetic structure and relationships between Italian sheep, 496 subjects from 19 breeds were typed at 50K single nucleotide polymorphism loci. A subset of foreign breeds from the Sheep HapMap dataset was also included in the analyses. Genetic distances (as visualized either in a network or in a multidimensional scaling analysis of identical by state distances) closely reflected geographic proximity between breeds, with a clear north–south gradient, likely because of high levels of past gene flow and admixture all along the peninsula. Sardinian breeds diverged more from other breeds, a probable consequence of the combined effect of ancient sporadic introgression of feral mouflon and long‐lasting genetic isolation from continental sheep populations. The study allowed the detection of previously undocumented episodes of recent introgression (Delle Langhe into the endangered Altamurana breed) as well as signatures of known, or claimed, historical introgression (Merino into Sopravissana and Gentile di Puglia; Bergamasca into Fabrianese, Appenninica and, to a lesser extent, Leccese). Arguments that would question, from a genomic point of view, the current breed classification of Bergamasca and Biellese into two separate breeds are presented. Finally, a role for traditional transhumance practices in shaping the genetic makeup of Alpine sheep breeds is proposed. The study represents the first exhaustive analysis of Italian sheep diversity in an European context, and it bridges the gap in the previous HapMap panel between Western Mediterranean and Swiss breeds. 相似文献
9.
10.
东灵山辽东栎虫损种子调查 总被引:2,自引:0,他引:2
2000年8月底至9月底,在北京东灵山地区小龙门林场对2块辽东栎样地中虫损种子下落动态进行了调查.结果表明:2块样地辽东栎种子产量分别高达19.15和91.23颗·m-2,而2块样地虫损种子较多,分别为49.23和33.38个·m-2,所占种子库比例分别为36.93%和25.96%;1999年研究发现(阎文杰,2000),2样地辽东栎种子产量为13.09和7.24颗·m-2,其中虫损种子产量为10.87和5.90颗·m-2,虫损率高达82.22%和75.43%.2年的观察结果均证实虫损种子在种子雨早期迅速下落,且低产年份的虫损种子在种子库中的比例显著高于高产年份.高比例的虫损种子可能是导致辽东栎实生苗更新率低的重要因素. 相似文献