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排序方式: 共有115条查询结果,搜索用时 15 毫秒
1.
刘大丽;鲁振强;张革艳;于婷婷;王旭 《植物研究》2011,31(6):692-695
为了阐明水稻Catalase(CAT)的酶学功能,首先需要获得出足量的、活性的该酶蛋白。本研究克隆了水稻OsCATB基因(GenBank accession No.D26484),构建到原核表达载体pGEX-6p-3中形成重组蛋白,继而转入E.coli菌株BL21中进行表达特性研究。结果表明,GST-OsCATB融合蛋白在E.coli中进行了过量表达,表达受到诱导剂浓度、诱导时间、诱导温度和诱导体系等多因素影响;通过谷胱甘肽Sepharose-4B亲合层析,纯化出足量、活性的融合蛋白GST-OsCATB,每克表达细胞(干重)中得率为51 mg GST-OsCATB。 相似文献
2.
[目的] 生物防治与化学防治相结合是害虫综合防治的重要策略,蛹寄生蜂白蛾周氏啮小蜂是一种重要的天敌昆虫,在美国白蛾的生物防控中起关键作用。本研究旨在评估农林生产中常用的广谱、低毒杀虫剂多杀菌素对白蛾周氏啮小蜂的安全性。[方法] 采用药膜法检测多杀菌素对小蜂的毒力,并检测亚致死浓度多杀菌素处理后,小蜂体内解毒酶活性的变化。[结果] 多杀菌素对小蜂无短期(1 h)触杀作用,但随施药时间延长,其对小蜂的毒杀作用增强,25 mg·L-1多杀菌素施药2 h后,可诱导约18.67%小蜂个体死亡。施药4 h后,不同浓度多杀菌素对小蜂的毒杀作用差异最明显,低浓度(5 mg·L-1)多杀菌素可诱导11.33%小蜂死亡,而中、高浓度多杀菌素(≥ 15 mg·L-1)可诱导77.33%~88.00%个体死亡。施药6 h后,LC50值达7.44 mg·L-1,安全系数为0.31,属高风险性农药。此外,亚致死浓度多杀菌素可明显诱导小蜂羧酸酯酶活性提高,对乙酰胆碱酯酶与谷胱甘肽-S-转移酶活性具有剂量效应。[结论] 多杀菌素对白蛾周氏啮小蜂的毒力作用较强,在美国白蛾生防区需慎用。 相似文献
3.
Different Responses of Plant Growth and Antioxidant System to the Combination of Cadmium and Heat Stress in Transgenic and Non-transgenic Rice 总被引:1,自引:0,他引:1
A comparative study of just cadmium (Cd) or heat and their combination treatments on some physiological parameters and the antioxidant systems in transgenic rice ( Oryza sativa L. cv. Zhonghua No.11) carrying glutathione-S-transferase (GST, EC. 2.5.1.18) and catalase1 (CAT1, EC. 1.11.1.6) and non-transgenics was conducted. The results revealed improved resistance in the transgenics to Cd and the combined Cd and heat stress than non-transgenics. Data showed that the activities of CAT, GST, superoxide dismutase (EC.1.15.1.1) and all components of the ascorbate-glutathione cycle measured in the stressed transgenics shoots are significantly different from those of non-transgenics. Results indicated that co-expression of GST and CAT1 had an important effect on the antioxidant system, in particular, the whole ascorbate-glutathione cycle. The less oxidative damage induced by Cd and the stress combination in the transgenics resulted not only from the GST and CAT1 transgene but also from the coordination of the whole ascorbate-glutathione cycle. 相似文献
4.
Heterooligomeric complexes formed by human small heat shock proteins HspB1 (Hsp27) and HspB6 (Hsp20)
Olesya V. Bukach Alisa E. Glukhova Alim S. Seit-Nebi Nikolai B. Gusev 《Biochimica et Biophysica Acta - Proteins and Proteomics》2009,1794(3):486-495
Formation of heterooligomeric complexes of human small heat shock proteins (sHsp) HspB6 (Hsp20) and HspB1 (Hsp27) was analyzed by means of native gel electrophoresis, analytical ultracentrifugation, chemical cross-linking and size-exclusion chromatography. HspB6 and HspB1 form at least two different complexes with apparent molecular masses 100–150 and 250–300 kDa, and formation of heterooligomeric complexes is temperature dependent. These complexes are highly mobile, easily exchange their subunits and are interconvertible. The stoichiometry of HspB1 and HspB6 in both complexes is close to 1/1 and smaller complexes are predominantly formed at low, whereas larger complexes are predominantly formed at high protein concentration. Formation of heterooligomeric complexes does not affect the chaperone-like activity of HspB1 and HspB6 if insulin or skeletal muscle F-actin was used as model protein substrates. After formation of heterooligomeric complexes the wild type HspB1 inhibits the rate of phosphorylation of HspB6 by cAMP-dependent protein kinase. The 3D mutant mimicking phosphorylation of HspB1 also forms heterooligomeric complexes with HspB6, but is ineffective in inhibition of HspB6 phosphorylation. Inside of heterooligomeric complexes HspB6 inhibits phosphorylation of HspB1 by MAPKAP2 kinase. Thus, in heterooligomeric complexes HspB6 and HspB1 mutually affect the structure of each other and formation of heterooligomeric complexes might influence diverse processes depending on small heat shock proteins. 相似文献
5.
6.
The very C-terminus of c-Src is a ligand for PDZ domains. In a screen for PDZ domains that interact with c-Src, we identified one of the PDZ domains of the Ligand-of-Numb protein X1 (LNX1), a multiple PDZ domain scaffold and RING type E3 ubiquitin ligase. We demonstrate that the interaction of c-Src with LNX1 depends on the C-terminal PDZ ligand of c-Src. Furthermore, we show that c-Src phosphorylates LNX1. Moreover, c-Src itself is ubiquitinated by LNX1, suggesting an interdependent regulation of c-Src and LNX1. 相似文献
7.
辣椒碱对小菜蛾的驱避活性及其体内谷胱甘肽-S-转移酶和Na+,K+-ATP酶活性的影响 总被引:3,自引:0,他引:3
采用常规生测方法和酶活力测定方法,初步研究了辣椒碱对小菜蛾Plutella xylostella L.的产卵忌避和拒食作用,及其对小菜蛾体内谷胱甘肽-S-转移酶、Na+,K+-ATP酶活性的影响,以期阐明辣椒碱对害虫的作用机制。结果表明,辣椒碱对小菜蛾表现出较强的产卵忌避活性和拒食活性。在6.25×104 mg/L浓度下,处理24 h辣椒碱对小菜蛾的非选择性产卵忌避率达96.55%,选择性产卵忌避率为84.30%;在相同浓度下,处理48 h辣椒碱对小菜蛾的非选择性拒食率达81.47%,选择性拒食率为69.69%。 另外,经1.25×105 mg/L辣椒碱不同时间处理后,小菜蛾体内的谷胱甘肽-S-转移酶酶活力和Na+,K+-ATP酶活力与对照相比均产生了波动,处理18 h时小菜蛾体内GSTs活力最高,为152.01 U·mg-1pro·min-1,处理1 h时小菜蛾体内Na+,K+-ATP酶活力最高,为19.99 U·mg-1pro·min-1。结果说明辣椒碱能够影响小菜蛾产卵和取食行为,并且对其体内的酶系也产生了影响。 相似文献
8.
9.
In humans, thromboxane (TX) A2 signals through the TPα and TPβ isoforms of its G-protein coupled TXA2 receptor (TP) to mediate a host of (patho)physiologic responses. Herein, angio-associated migratory cell protein (AAMP) was identified as a novel interacting partner of both TPα and TPβ through an interaction dependent on common (residues 312-328) and unique (residues 366-392 of TPβ) sequences within their carboxyl-terminal (C)-tail domains. While the interaction was constitutive in mammalian cells, agonist-stimulation of TPα/TPβ led to a transient dissociation of AAMP from immune complexes which coincided with a transient redistribution of AAMP from its localization in an intracellular fibrous network. Although the GTPase RhoA is a downstream effector of both AAMP and the TPs, AAMP did not influence TP-mediated RhoA or vice versa. Small interfering RNA (siRNA)-mediated disruption of AAMP expression decreased migration of primary human coronary artery smooth muscle cells (1° hCoASMCs). Moreover, siRNA-disruption of AAMP significantly impaired 1° hCoASMC migration in the presence of the TXA2 mimetic U46619 but did not affect VEGF-mediated cell migration. Given their roles within the vasculature, the identification of a specific interaction between TPα/TPβ and AAMP is likely to have substantial functional implications for vascular pathologies in which they are both implicated. 相似文献
10.
P. PalsamyS. Subramanian 《生物化学与生物物理学报:疾病的分子基础》2011,1812(7):719-731
Hyperglycemia-mediated oxidative stress plays a crucial role in the progression of diabetic nephropathy. Hence, the present study was hypothesized to explore the renoprotective nature of resveratrol by assessing markers of oxidative stress, proinflammatory cytokines and antioxidant competence in streptozotocin-nicotinamide-induced diabetic rats. Oral administration of resveratrol to diabetic rats showed a significant normalization on the levels of creatinine clearance, plasma adiponectin, C-peptide and renal superoxide anion, hydroxyl radical, nitric oxide, TNF-α, IL-1β, IL-6 and NF-κB p65 subunit and activities of renal aspartate transaminase, alanine transaminase and alkaline phosphatase in comparison with diabetic rats. The altered activities of renal aldose reductase, sorbitol dehydrogenase and glyoxalase-I and elevated level of serum advanced glycation end products in diabetic rats were also reverted back to near normalcy. Further, resveratrol treatment revealed a significant improvement in superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase activities and vitamins C and E, and reduced glutathione levels, with a significant decline in lipid peroxides, hydroperoxides and protein carbonyls levels in diabetic kidneys. Similarly, mRNA and protein analyses substantiated that resveratrol treatment notably normalizes the renal expression of Nrf2/Keap1and its downstream regulatory proteins in the diabetic group of rats. Histological and ultrastructural observations also evidenced that resveratrol effectively protects the kidneys from hyperglycemia-mediated oxidative damage. These findings demonstrated the renoprotective nature of resveratrol by attenuating markers of oxidative stress in renal tissues of diabetic rats. 相似文献