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黄土丘陵区草地土壤微生物C、N及呼吸熵对植被恢复的响应 总被引:6,自引:0,他引:6
以野外样地调查和室内分析法研究了黄土丘陵区不同植被恢复年限下草地土壤微生物C、N及土壤呼吸熵的变化.结果表明,土壤微生物量碳明显地随着植被恢复年限的增加而增加.在恢复前23a, 土壤微生物量碳在0~20 cm土层年增加率为24.1%;20~40 cm为104.4%.植被恢复23a后,0~20 cm土层增长率为0.83%,20~40 cm为0.19%.土壤微生物量N表现为在植被恢复的初期略有下降,3a后,开始出现明显增加.0~20 cm土层年增长率为20.14%,20~40 cm为15.11%.在植被恢复23a后,0~20 cm土层的年增长率为0.14%,20~40 cm变化不大.土壤微生物呼吸强度随着恢复年限的增加逐渐加强;土壤呼吸熵随植被封育时间的增加而呈对数降低趋势.土壤呼吸熵(qCO2)在反映土壤的生物质量变化时,显得更加稳定,受植物生长状况影响较小.相关分析表明,土壤微生物量和土壤微生物活性与土壤有机质、碱解氮和粘粒含量显著正相关;与土壤粉粒含量明显负相关;表层土壤pH值对其也有明显影响.草地植被自然恢复过程可增加土壤微生物活性,有利于土壤质量的提高. 相似文献
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树麻雀代谢率和器官重量在光周期驯化中的变化 总被引:4,自引:2,他引:2
为探讨光周期对树麻雀代谢产热和代谢器官变化的影响,对成年树麻雀 Passer montanus 进行温度为25℃、两种光周期16 L:8 D(LD组)和8 L:16 D(SD组)4周的气候箱驯化后,测定其体重、基础代谢率(BMR)、体脂和水分含量,以及各器官、组织的鲜重和干重.结果 显示,SD组麻雀BMR显著大于LD组,作为代谢活性器官的消化道显著增生是其BMR显著增加的主要原因之一;麻雀通过增加体重和能量储备物脂肪来实现对较长黑暗环境的适应是其成功的生存策略.SD组麻雀体重的显著增加和水分含量显著降低,说明短光照的刺激引起麻雀能量储备的增加和代谢活性器官消化道增生.光周期驯化的结果验证了"中心限制假说",即麻雀体内存在着与BMR相关的"代谢机器",中心器官(消化道)是改变麻雀BMR的基础之一. 相似文献
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Yoshiaki Hirako Yuki Yonemoto Tomoe Yamauchi Yuji Nishizawa Yoshiyuki Kawamoto Katsushi Owaribe 《Experimental cell research》2014
Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of hemidesmosomes was observed when these cells were cultured for more than 10 days in a commercial serum-free medium without supplemental calcium. Electron microscopy demonstrated that numerous electron-dense adhesion structures were present along the basal cell membranes of DJM-1 cells cultured under the aforementioned conditions. After removing cellular materials using an ammonia solution, hemidesmosomal proteins and deposited extracellular matrix were collected and separated by electrophoresis. There were eight major polypeptides, which were determined to be plectin, BP230, BP180, integrin α6 and β4 subunits, and laminin-332 by immunoblotting and mass spectrometry. Therefore, we designated this preparation as a hemidesmosome-rich fraction. This fraction contained laminin-332 exclusively in its unprocessed form, which may account for the promotion of laminin deposition, and minimal amounts of Lutheran blood group protein, a nonhemidesmosomal transmembrane protein. This hemidesmosome-rich fraction would be useful not only for biological research on hemidesmosomes but also for developing a serum test for patients with blistering skin diseases. 相似文献
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Fungal‐host diversity among mycoheterotrophic plants increases proportionally to their fungal‐host overlap 下载免费PDF全文
Sofia I. F. Gomes Vincent S. F. T. Merckx Serguei Saavedra 《Ecology and evolution》2017,7(10):3623-3630
The vast majority of plants obtain an important proportion of vital resources from soil through mycorrhizal fungi. Generally, this happens in exchange of photosynthetically fixed carbon, but occasionally the interaction is mycoheterotrophic, and plants obtain carbon from mycorrhizal fungi. This process results in an antagonistic interaction between mycoheterotrophic plants and their fungal hosts. Importantly, the fungal‐host diversity available for plants is restricted as mycoheterotrophic interactions often involve narrow lineages of fungal hosts. Unfortunately, little is known whether fungal‐host diversity may be additionally modulated by plant–plant interactions through shared hosts. Yet, this may have important implications for plant competition and coexistence. Here, we use DNA sequencing data to investigate the interaction patterns between mycoheterotrophic plants and arbuscular mycorrhizal fungi. We find no phylogenetic signal on the number of fungal hosts nor on the fungal hosts shared among mycoheterotrophic plants. However, we observe a potential trend toward increased phylogenetic diversity of fungal hosts among mycoheterotrophic plants with increasing overlap in their fungal hosts. While these patterns remain for groups of plants regardless of location, we do find higher levels of overlap and diversity among plants from the same location. These findings suggest that species coexistence cannot be fully understood without attention to the two sides of ecological interactions. 相似文献
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It is generally assumed in dendroecological studies that annual tree-ring growth is adequately determined by a linear function of local or regional precipitation and temperature with a set of coefficients that are temporally invariant. However, various researchers have maintained that tree-ring records are the result of multivariate, often nonlinear biological and physical processes. To describe critical processes linking climate variables with tree-ring formation, the process-based tree-ring Vaganov–Shashkin model (VS-model) was successfully used. However, the VS-model is a complex tool requiring a considerable number of model parameters that should be re-estimated for each forest stand. Here we present a new visual approach of process-based tree-ring model parameterization (the so-called VS-oscilloscope) which allows the simulation of tree-ring growth and can be easily used by researchers and students. The VS-oscilloscope was tested on tree-ring data for two species (Larix gmeliniiand Picea obovata) growing in the permafrost zone of Central Siberia. The parameterization of the VS-model provided highly significant positive correlations (p < 0.0001) between simulated growth curves and original tree-ring chronologies for the period 1950–2009. The model outputs have shown differences in seasonal tree-ring growth between species that were well supported by the field observations. To better understand seasonal tree-ring growth and to verify the VS-model findings, a multi-year natural field study is needed, including seasonal observation of the thermo-hydrological regime of the soil, duration and rate of tracheid development, as well as measurements of their anatomical features. 相似文献
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Over the past two decades, the dendroclimate community has produced various annually resolved, warm season temperature reconstructions for the extratropical Northern Hemisphere. Here we compare these tree-ring based reconstructions back to 831 CE and present a set of basic metrics to provide guidance for non-specialists on their interpretation and use. We specifically draw attention to (i) the imbalance between (numerous) short and (few) long site chronologies incorporated into the hemispheric means, (ii) the beneficial effects of including maximum latewood density chronologies in the recently published reconstructions, (iii) a decrease in reconstruction covariance prior to 1400 CE, and (iv) the varying amplitudes and trends of reconstructed temperatures over the past 1100 years. Whereas the reconstructions agree on several important features, such as warmth during medieval times and cooler temperatures in the 17th and 19th centuries, they still exhibit substantial differences during 13th and 14th centuries. We caution users who might consider combining the reconstructions through simple averaging that all reconstructions share some of the same underlying tree-ring data, and provide four recommendations to guide future efforts to better understand past millennium temperature variability. 相似文献