全文获取类型
收费全文 | 643篇 |
免费 | 27篇 |
国内免费 | 79篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 15篇 |
2019年 | 11篇 |
2018年 | 13篇 |
2017年 | 14篇 |
2016年 | 18篇 |
2015年 | 17篇 |
2014年 | 33篇 |
2013年 | 39篇 |
2012年 | 39篇 |
2011年 | 38篇 |
2010年 | 33篇 |
2009年 | 44篇 |
2008年 | 49篇 |
2007年 | 49篇 |
2006年 | 40篇 |
2005年 | 44篇 |
2004年 | 37篇 |
2003年 | 34篇 |
2002年 | 24篇 |
2001年 | 20篇 |
2000年 | 15篇 |
1999年 | 11篇 |
1998年 | 16篇 |
1997年 | 8篇 |
1996年 | 15篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 9篇 |
1984年 | 3篇 |
1982年 | 5篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有749条查询结果,搜索用时 15 毫秒
1.
We describe the development of DNA markers for the fungal pathogen of Eucalyptus, Cryphonectria cubensis. These markers originated from cloned intershort sequence repeat polymerase chain reactions, which enrich for medium to highly repetitive DNA sequences. In total, 10 markers were isolated, eight of which were polymorphic, and these can subsequently be applied to study populations of C. cubensis. 相似文献
2.
3.
DAVID WEETMAN LORENZ HAUSER PAUL W. SHAW MICHELLE K. BAYES 《Molecular ecology resources》2005,5(2):361-362
Five microsatellite loci are described for the commercially exploited marine gastropod, Buccinum undatum. Levels of polymorphism were variable with three to 19 alleles per locus and expected heterozygosities of 0.26–0.94 in 60 individuals of the population from which the loci were isolated. Homozygote excess at two of the loci might be attributable to null alleles, and these loci should not be used in, for example, parentage analysis. Nevertheless, because null allele frequencies can be estimated and their effects partitioned, all are useful markers for studies of population differentiation. 相似文献
4.
5.
Universal primers for amplification of three non-coding regions of chloroplast DNA 总被引:90,自引:0,他引:90
Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked for most species tested including algae, bryophytes, pteridophytes, gymnosperms and angiosperms. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology (in supplying markers) and evolution (inter- and probably intraspecific phylogenies) of plants. 相似文献
6.
7.
T. Yoshimura S. Ebihara 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1996,178(6):797-802
Light-dark cycles are the most important time cue for the circadian system to entrain the endogenous circadian clock to the environmental 24 h cycle. Although photic entrainment of circadian rhythms is mediated by the eye in mammals, photoreceptors implicated in circadian photoreception remain unknown. In our previous study, retinally degenerate CBA/J (rd/rd) mice were found to have lower circadian photo-sensitivity for phase-shifting the locomotor activity rhythms than normal CBA/N(+/+) mice. In the present study, the spectral sensitivity for phase-shifting the rhythms was examined in order to characterize the photopigments involved in circadian photoreception of these mice. The spectral sensitivity of CBA/J-rd/rd mice clearly fitted to the Dartnall nomogram for a retinal1-based pigment with a maximum at 480 nm, while the best fitted nomogram had a maximum at 500 nm in CBA/N- +/+ mice. These results suggest that circadian photopigments involved in CBA/J-rd/rd and CBA/N- +/+ mice may be different. 相似文献
8.
9.
Jeffrey A. Banas Daniel Simon Lisa K. Williams Joseph J. Ferretti Roy R.B. Russell 《FEMS microbiology letters》1994,123(3):349-354
Abstract A glucosyltransferase (GTF) gene, designated gtfL , from Streptococcus salivarius was cloned and expressed in Escherichia coli and its nucleotide sequence determined. The GTF-L enzyme catalysed the synthesis of water-insoluble glucan in a primer-independent manner. The nucleotide sequence and derived amino acid sequence of GTF-L were similar in size and domain structure to previously sequenced glucosyltransferases. However, a 464-bp region of high variability was identified which could be selectively amplified from strains of S. salivarius by the polymerase chain reaction and could therefore form the basis for species identification. No sequence-specific motifs related to the solubility and linkage of the glucan product or its need for a dextran primer could be ascertained. 相似文献
10.