Effect of calf removal on serum luteinizing hormone and cortisol concentrations in postpartum beef cows

Serum luteinizing hormone (LH) and cortisol concentrations were measured in ten fall calving, Angus cows averaging 38 +/- 8 days postpartum. Calves from five cows were weaned at the beginning of the study. Blood samples were collected at 20 min. intervals for 48 h after weaning and for 8 h on day 4 and day 6 postweaning. Mean serum LH concentrations increased (P<0.01) in weaned cows (W) from 0.55 +/- 0.01 ng/ml at time of calf removal to 1.3 +/- 0.04 ng/ml 48 h afterwards. Comparable LH concentrations for suckled cows (S) were 0.65 +/- 0.08 ng/ml and 0.62 +/- 0.03 ng/ml respectively. Average serum LH concentrations at 48 h after weaning were greater (P<0.01) for W cows than S cows and a treatment by time interaction occurred (P<0.01) with serum LH concentrations increasing (P<0.01) from time of calf removal to 48 h after calf removal in W cows. Frequency of LH peaks increased (P<0.01) in W cows and by 48 h after weaning was greater (P<0.01) in W cows than in S cows. Magnitude of LH peaks did not differ between the two groups. Serum cortisol concentrations were not different between W and S cows except for a transient elevation (P<0.01) in W cows from 7.6 +/- 0.9 ng/ml to 11.9 +/- 1.0 ng/ml 9 to 12 h after calf removal. Since serum LH concentrations were increased in W cows but not in S cows at 48 h and serum cortisol concentrations increased transiently in W cows we suggest that circulating cortisol levels may not be a physiological inhibitor of LH secretion in the suckled postpartum beef cow.     

Release of PGF(2alpha) during parturition and the postpartum period in the ewe

Peripheral plasma levels of 15-keto-13, 14-dihydro-PGF(2alpha) were determined from one week before parturition until five weeks postpartum in six ewes. Starting at approximately 1 nmol/l one week before parturition, the prostaglandin metabolite levels rose successively during the week preceding parturition and peaked at 70 to 125 nmol/l during parturition. During the first three days postpartum the concentrations of the 15-keto-13, 14-dihydro-PGF(2alpha) decreased to 0.5 to 1 nmol/l. These levels were maintained for three weeks and low levels of 50 to 100 pmol/l were reached around four weeks postpartum. The ewe, as well as other ruminants, has a sustained release of elevated concentrations of PGF(2alpha) postpartum. It seems likely that PGF(2alpha) plays a role in uterine involution.     

The level of pancreatic PLA2 receptor is closely associated with the proliferative state of rat uterine stromal cells

Rat uterine stromal cells (U_III) express pancreatic type PLA₂ (PLA₂-I) receptor and internalize the enzyme bound to receptors. Here, we investigate the proliferating effect and alterations in binding of PLA₂-I. There is a dramatic decline in PLA₂-I binding in U_III cells as they progress from a nonconfluent proliferating state (40,000 sites/cell) to a confluent state (1300 sites/cell). Intracellular concentration of PLA₂-I changed with the alteration in binding, suggesting that regulation in the PLA₂ binding capacity may have important implications in growth control mechanisms.     

小鼠胚胎与子宫单层上皮细胞共培养的研究

本文报道建立了小鼠胚胎与小鼠子宫单层上皮细胞体外共培养系统。结果揭示;小鼠胚胎与 子宫单层上皮细胞共培养可以促进胚胎的发育、粘附和扩展;如果培养液中加入 ３、６７ × １０－６ｍｏｌ／Ｌ １７β－雌二醇,可以显著提高胚胎在共培养系统中的发育率、粘附率和扩展率。以上结果表明：小鼠 胚胎与小鼠子宫单层上皮细胞共培养系统是研究胚泡着床机理较理想的研究手段。     

凝血-纤溶失衡与子痫前期的关系及对产后大出血的预测价值研究

摘要 目的：分析凝血-纤溶失衡与子痫前期的关系及对产后大出血的预测价值。方法：选择我院自2018年1月至2021年12月接诊的160例子痫前期孕妇作为观察组,另选同期的160例健康妊娠孕妇作为对照组;以凝血酶-抗凝血酶复合物（TAT）/纤溶酶-α2纤溶酶抑制物复合物（PIC）比值评价凝血-纤溶失衡程度,使用Pearson相关性分析TAT/PIC比值与分娩孕周、分娩出血量的关系,通过AUC评价TAT/PIC比值对产后大出血的预测效能。结果：观察组血浆TAT水平高于对照组,PIC水平低于对照组,TAT/PIC比值大于对照组（P<0.05）;经Pearson相关性分析,子痫前期孕妇TAT/PIC比值与分娩出血量呈正相关,与出生体重呈负相关（P<0.05）;产后大出血组血浆TAT水平高于非产后大出血组,PIC水平低于非产后大出血组,TAT/PIC比值大于非产后大出血组（P<0.05）;经ROC曲线分析,TAT/PIC比值预测子痫前期孕妇产后大出血的AUC为0.910,大于TAT的0.665和PIC的0.650（P<0.05）。结论：子痫前期的发生可能与凝血-纤溶失衡有关,而TAT/PIC比值与分娩出血量及出生体重的关系密切,预测产后大出血的效能较好,值得临床予以重视应用。     

颅内压参数联合血清caveolin-1、AQP-4对高血压脑出血患者术后预后不良的预测价值

摘要 目的：探讨颅内压参数联合血清小窝蛋白-1（caveolin-1）、水通道蛋白4（AQP-4）对高血压脑出血（HICH）患者术后预后不良的预测价值。方法：选择2020年1月至2022年1月河北省胸科医院收治的106例HICH患者,术后随访3个月,根据格拉斯哥预后（GOS）评分将患者分为预后良好组（55例）,预后不良组（51例）。术后监测颅内压参数[压力反应指数（PRx）、平均颅内压波幅（MWA）、20 mmHg阈值下颅内压剂量（Dicp20）],检测血清caveolin-1、AQP-4水平。多因素Logistic回归分析HICH患者术后预后不良的因素。受试者工作特征曲线（ROC）分析颅内压参数联合血清caveolin-1、AQP-4预测HICH患者术后预后不良的价值。结果：预后不良组PRx、MWA、Dicp20以及血清caveolin-1、AQP-4水平高于预后良好组（P＜0.05）。低术前格拉斯哥昏迷评分（GCS）评分、高PRx、高Dicp20、高caveolin-1、高AQP-4是HICH患者术后预后不良的危险因素（P＜0.05）。联合PRx、Dicp20、caveolin-1和AQP-4预测HICH患者术后3个月预后不良的的曲线下面积为0.823,大于PRx、Dicp20、caveolin-1和AQP-4单独预测。结论：高PRx、Dicp20、caveolin-1、AQP4是HICH患者术后预后不良的危险因素,联合颅内压参数PRx、Dicp20及血清caveolin-1、AQP4预测HICH患者术后预后不良具有较高的价值。