松龄血脉康胶囊对局灶性缺血损伤后大鼠脑组织TNF-α表达的影响

目的：探讨松龄血脉康预处理对大鼠急性局灶性脑缺血再灌注损伤脑组织TNF-α表达的影响。方法：36只雄性SD大鼠,随机分为松龄血脉康（SL-xmk）预处理组、假手术组、对照组,SL-xmk预处理组采用SL-xmk（937.5mg/kg）悬浮液对大鼠进行4w预防性灌胃处理,假手术组、对照组采用等容量生理盐水预防性灌胃处理,在预处理终点采用线栓法制作大鼠大脑中动脉阻塞（middlecerebralarteryocclusion,MCAO）模型。观察SL-xmk预处理后MCAO大鼠脑组织含水量、脑梗死体积变化的影响,运用免疫组织化学法检测大鼠缺血脑组织TNF-α免疫反应阳性细胞表达。结果：SL-xmk预处理后脑组织TNF-α表达显著下降,缺血脑组织含水量和脑梗死体积明显降低。结论：松龄血脉康可抑制急性局灶性脑缺血再灌注损伤后脑组织TNF-α的表达。     

松龄血脉康胶囊联合立普妥对老年高血压患者血清Fibulin-3、Lp(a)、MCP-1水平的影响

目的:研究松龄血脉康胶囊联合立普妥对老年高血压患者血清细胞外基质蛋白-3 (Fibulin-3)、脂蛋白a (Lipoprotein(a),Lp(a))、单核细胞趋化蛋白-1 (monocyte chemoattractant protein-1,MCP-1)水平的影响。方法:选择2015年04月至2017年12月在我院治疗的老年高血压患者72例,按照随机数字表法为观察组和对照组,对照组采用立普妥口服治疗,观察组在对照组的基础上联用松龄血脉康胶囊口服治疗,观察和比较两组临床治疗效果,治疗前后血脂、动态血压及Fibulin-3、Lp(a)、MCP-1水平的变化情况。结果:治疗后,观察组总有效率为88.89%,明显高于对照组(69.44%,P0.05);观察组血清血清总胆固醇(Serum total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、甘油三酯(triglyceride,TG)、游离脂肪酸(nonesterified fatty acid,FFA)、Lp(a)、MCP-1等水平均显著低于对照组(P0.05),而血清HDL-C、Fibulin-3水平明显高于对照组(P0.05);治疗后,观察组动态血压水平降低范围较对照组更明显(P0.05);观察组患者生活愉快、心理健康及认知功能等方面评分均显著高于对照组(P0.05)。结论:松龄血脉康胶囊联合立普妥治疗老年高血压患者的临床疗效显著优于单用立普妥口服治疗,其能更有效维持动态血压的正常水平,且显著改善Fibulin-3、Lp(a)、MCP-1水平,降脂效果良好。     

Progress and perspective on cyanobacterial glycogen metabolism engineering

As important oxygenic photoautotrophs, cyanobacteria are also generally considered as one of the most promising microbial chassis for photosynthetic biomanufacturing. Diverse synthetic biology and metabolic engineering approaches have been developed to enable the efficient harnessing of carbon and energy flow toward the synthesis of desired metabolites in cyanobacterial cell factories. Glycogen metabolism works as the most important natural carbon sink mechanism and reserve carbon source, storing a large portion of carbon and energy from the Calvin-Benson-Bassham (CBB) cycle, and thus is traditionally recognized as a promising engineering target to optimize the efficacy of cyanobacterial cell factories. Multiple strategies and approaches have been designed and adopted to engineer glycogen metabolism in cyanobacteria, leading to the successful regulation of glycogen synthesis and storage contents in cyanobacteria cells. However, disturbed glycogen metabolism results in weakened cellular physiological functionalities, thereby diminishing the robustness of metabolism. In addition, the effects of glycogen removal as a metabolic engineering strategy to enhance photosynthetic biosynthesis are still controversial. This review focuses on the efforts and effects of glycogen metabolism engineering on the physiology and metabolism of cyanobacterial chassis strains and cell factories. The perspectives and prospects provided herein are expected to inspire novel strategies and tools to achieve ideal control over carbon and energy flow for biomanufacturing.