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Kayla J. Vastenhout Ruthellen H. TornbergAmanda L. Johnson Michael W. AmolinsJared R. Mays 《Analytical biochemistry》2014
Isothiocyanates (ITCs) are one of several hydrolysis products of glucosinolates, plant secondary metabolites that are substrates for the thioglucohydrolase myrosinase. Recent pursuits toward the development of synthetic non-natural ITCs have consequently led to an exploration of generating these compounds from non-natural glucosinolate precursors. Evaluation of the myrosinase-dependent conversion of select non-natural glucosinolates to non-natural ITCs cannot be accomplished using established ultraviolet–visible (UV–Vis) spectroscopic methods. To overcome this limitation, an alternative high-performance liquid chromatography (HPLC)-based analytical approach was developed where initial reaction velocities were generated from nonlinear reaction progress curves. Validation of this HPLC method was accomplished through parallel evaluation of three glucosinolates with UV–Vis methodology. The results of this study demonstrate that kinetic data are consistent between both analytical methods and that the tested glucosinolates respond similarly to both Michaelis–Menten and specific activity analyses. Consequently, this work resulted in the complete kinetic characterization of three glucosinolates with Sinapis alba myrosinase, with results that were consistent with previous reports. 相似文献
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本实验室在卫生毒理学研究生培养中开展Journal Club和Progress Discussion教学模式,使研究生耳濡目染地学习国际同行的高水平研究,开阔科研视野,带着问题去进行研究,并及时进行总结提高,旨在提高研究生科研选题、文献获取、实践操作、英文阅读与使用等综合能力。5年的实践证明,Journal Club和Progress Discussion取得了很好的效果。 相似文献
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口蹄疫疫苗研究新进展 总被引:1,自引:0,他引:1
口蹄疫是世界性重大动物疫病之一.接种疫苗是预防该病的重要手段之一,而研制高质量、安全有效的疫苗.不但是决定疫苗免疫效果的关键,也是成功预防、控制直至最终消灭口蹄疫的先决条件.目前,除传统疫苗仍然在口蹄疫防控中扮演重要角色以外,国内外诸如亚单位疫苗、载体疫苗、核酸疫苗、基因缺失疫苗、合成肽疫苗、可饲疫苗和多表位疫苗等的研究和探索已全面展开,有望为口蹄疫的有效防控提供新的途径. 相似文献
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中国境内分布着数千处古人类活动遗址,是早期人类演化与适应行为研究的重要区域。虽然以往的研究一直关注地学手段在解读遗址地貌和地层形成过程中的作用,然而,作为正确鉴别遗址完整性和早期人类生存行为研究的遗址形成过程(或遗址成因)分析,长期以来缺少系统性的研究和关键指标的选择。本文结合古人类活动遗址保存的地貌和主要沉积物类型,依据河湖相沉积、洞穴(岩厦、裂隙)发育区、黄土与红土分布区等类型中遗址的特点,回顾近10年来我国在该领域的研究进展。通过对不同分布区遗址的地貌和沉积特点的介绍,结合重要遗址的形成过程研究的进展,总结不同分布区遗址埋藏类型对研究视角与分析指标的选择差异。在上述基础上,作者对目前我国遗址成因研究现状、存在问题和未来研究方向进行讨论,为该领域研究提供参考信息。 相似文献
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近年来IL-13受体IL-13Rα2的研究已经成为一个新兴热点,IL-13Rα2在许多肿瘤细胞细胞表面存在特异性高表达,而在正常组织中不表达或表达量很低,通过IL-13Rα2介导的毒素融合蛋白靶向治疗肿瘤也已成为肿瘤诊断和免疫治疗研究中的重要方面。随着对IL-13Rα-结构和功能及其与肿瘤关系研究的不断深入,将为肿瘤的靶向治疗提供新的思路,为肿瘤的临床治疗提供新的理论依据。对IL-13Rα2在不同肿瘤中的表达及IL-13和不同毒素融合得到的毒素融合蛋白在细胞和动物体内等不同水平上对肿瘤治疗的作用做出了相应概括,并对通过IL-13Rα2介导的毒素融合蛋白治疗肿瘤机理的研究以及融合蛋白方法改进及其它相关治疗方法等方面作出综述。 相似文献
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2000~2005年《生物化学与生物物理进展》载文被引分析 总被引:1,自引:0,他引:1
依据美国《科学引文索引(扩展版)》(ScienceCitationIndexExpanded,SCIE)和期刊引证报告(JournalCitationReports,JCR),采用文献计量方法对2000~2005年《生物化学与生物物理进展》载文被引用情况进行统计分析及评价.该刊论文平均单篇被引次数为1.70次,被引作者分布于国内23个省(直辖市)和部分国外作者,北京、湖南和广东发表在《生物化学与生物物理进展》上的论文被引比率较高.在被引机构中来自中南大学湘雅医学院的论文被引频次最高.共有115种期刊引用《生物化学与生物物理进展》. 相似文献
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Marko Golinik 《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):391-399
The kinetic behaviour of insect acetylcholinesterases deviates from the Michaelis-Menten pattern. These deviations are known as activation or inhibition at various substrate concentrations and can be more or less observable depending on mutations around the active site of the enzyme. Most kinetic studies on these enzymes still rely on initial rate measurements. It is demonstrated here that according to this method one of the deviations can be overlooked. We attempt to point out that in such cases a detailed step-by-step progress curves analysis is successful. The study is focused on two different methods of analysing progress curves: (i) the first one is based on an integrated initial rate equation which can sufficiently fit truncated progress curves under corresponding conditions; and (ii) the other one precludes the algebraic formulae, but uses numerical integration for searching a non analytical solution of ordinary differential equations describing a kinetic model. All methods are tested on three different acetylcholinesterase mutants from Drosophila melanogaster. The results indicate that kinetic parameters for the E107K mutant with highly expressive activation and inhibition can be well evaluated applying any analysis method. It is quite different for E107W and E107Y mutants where latent activation is present, but discovered only using one or the other progress curves analysis methods. 相似文献
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双向电泳技术进展及在动物和植物科学研究中的应用 总被引:3,自引:0,他引:3
综述了双向电泳技术的原理、每一步骤的研究进展及其在动物和植物科学研究中的应用。 相似文献