全文获取类型
收费全文 | 292篇 |
免费 | 1篇 |
国内免费 | 12篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2017年 | 2篇 |
2015年 | 2篇 |
2014年 | 4篇 |
2012年 | 5篇 |
2011年 | 6篇 |
2010年 | 2篇 |
2009年 | 8篇 |
2008年 | 7篇 |
2007年 | 7篇 |
2006年 | 1篇 |
2005年 | 4篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 8篇 |
1998年 | 7篇 |
1997年 | 5篇 |
1996年 | 13篇 |
1995年 | 4篇 |
1994年 | 11篇 |
1993年 | 8篇 |
1992年 | 7篇 |
1991年 | 6篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 16篇 |
1987年 | 8篇 |
1986年 | 16篇 |
1985年 | 16篇 |
1984年 | 19篇 |
1983年 | 7篇 |
1982年 | 13篇 |
1981年 | 13篇 |
1980年 | 6篇 |
1979年 | 11篇 |
1978年 | 7篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1974年 | 1篇 |
排序方式: 共有305条查询结果,搜索用时 31 毫秒
1.
2.
K. Sasikala Ch.V. Ramana P. Raghuveer Rao M. Subrahmanyam 《FEMS microbiology letters》1990,72(1-2):23-28
Abstract Photoproduction of hydrogen, nitrogenase activity (acetylene reduction) and hydrogenase activity (methylene blue dye reduction) were studied in free and alginate immobilized whole cells of a purple non-sulfur photosynthetic bacterium Rhodobacter sphaeroides O.U. 001. Four-fold increase in hydrogen production, two-fold increase in nitrogenase activity and 1.2-fold increase in the hydrogenase activity were observed in immobilized cells compared to free cells. Effect of various inhibitors (CO and C2 H2 ) and electron donor (H2 ) on the above three functions by free and immobilized cells has also been studied. 相似文献
3.
4.
5.
Hydrogen metabolism of the unicellular cyanobacterium Chroococcidiopsis thermalis ATCC29380 总被引:1,自引:0,他引:1
Abstract Hydrogenase was induced in the unicellular cyanobacterium Chroococcidiopsis thermalis ATCC29380 when grown aerobically in a medium lacking combined nitrogen. Nitrogenase, however, was only observed after incubation of cells in a microaerobic environment. Hydrogen evolution could not be detected under aerobic conditions, but upon transfer of cells to dark anaerobic conditions, large amounts of hydrogen were immediately produced. This hydrogen evolution was sensitive to light and oxygen but not to inhibitors of protein synthesis. The enzyme activity catalyzing the formation of hydrogen was not membrane-bound; some functional properties were characterized in cell-free extracts. 相似文献
6.
Abstract In Trichodesmium contortum , nitrogenase was detected in only a limited number (about 10%) of microscopically distinguishable, consecutively arranged cells in central regions of the trichomes. Cells with nitrogenase also contained the photosystem II associated pigment phycoerythrin. These cells were not distinguishable from other cells on a structural basis, but were clearly visible at low magnification microscopy as all in the zone were more compact and shorter than those on either side. The compartmentalisation of nitrogenase into a chain of cells and in a possibly photosynthetic environment represents a previously undescribed phenomenon. The nitrogenase containing cells apparently perform the O2 protective function of heterocysts yet are different in several aspects. 相似文献
7.
【目的】以广西岑溪市野生稻保护区的药用野生稻为材料,分离纯化内生细菌,筛选固氮酶活性较高和对作物促生效果较好的菌株。【方法】利用乙炔还原法检测固氮酶活性,采用SDS-PAGE全细胞蛋白电泳和IS-PCR指纹图谱技术对分离到的固氮菌进行聚类。利用16S rRNA基因和nifH基因确定其系统发育地位。采用钼锑抗比色法、Salkowski比色法和CAS检测法分别测定菌株溶磷性、生长素的分泌能力和产铁载体能力。通过平板和盆栽试验检测其对水稻的促生作用。【结果】共分离得到35株内生固氮菌,分为6个类群。其中CX24固氮酶活性最高,经鉴定属于Klebsiella variicola,其固氮酶活性为298.64 μmol/(L·h),为参比模式菌株DSM15968的9倍。另外该菌株还具有较高的溶磷性、分泌生长素和产铁载体能力,能够有效地促进水稻的萌发和生长。【结论】菌株CX24属于Klebsiella variicola,是一株高效内生固氮菌,具有很好的生产应用前景。 相似文献
8.
类芦和狗牙根内生固氮菌初步研究 总被引:1,自引:0,他引:1
为探索水土保持禾草的固氮能力,利用乙炔还原法(ARA)对类芦(Neyraudia reynaudiana)和狗牙根(Cynodon dactylon)植株内生固氮菌的固氮酶活性进行研究.结果表明:在类芦茎部和狗牙根叶片中存在高固氮酶活性的内生固氮菌群;这些菌群在无氮培养基上经划线分离纯化得到40个菌株,其中26株有微弱固氮酶活性,14株检测不到固氮酶活性;从类芦茎部和狗牙根叶片中筛选到的固氮菌群在兼性厌氧的环境中有较高的固氮酶活性,且在石蜡油密封条件下,两者的固氮酶活性均最高;在不改变自然界微生物之间的协同关系情况下,混合菌群均具有较高的固氮酶活性,而人为组配混合得到的菌群固氮酶活性较低. reynaudiana)和狗牙根(Cynodondxtylon)植株内生固氮菌的固氮酶活性进行研究.结果表明:在类芦茎部和狗牙根叶片中存在高固氮酶活性的内生固氮菌群;这些菌群在无氮培养基上经划线分离纯化得到40个菌株,其中26株有微弱固氮酶活性,14株检测不到固氮酶活性;从类芦茎部和狗牙根叶片中筛选到的固氮菌群在兼性厌氧的环境中有较高的固氮酶活性,且在石蜡油密封条件下,两者的固氮酶活性均最高;在不改变自然界微生物之间的协同关系 况下,混合菌群均具有较高的固氮酶 相似文献
9.
Gabriela García-Esquivel Graciano Calva-Calva Ronald Ferrera-Cerrato Luis Carlos Fernández-Linares Refugio Rodríguez Vázquez Fernando José Esparza-García 《Archives of microbiology》2009,191(3):275-281
Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in
comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was
higher and the poly-β-hydroxybutyrate granules were not present to a significant extent, peripheral bodies were abundant.
After 8 days of culture on kerosene, the presence of cysts with intracellular bunches of poly-β-hydroxybutyrate granules was
observed. Germination of cysts bears germinating multicelled yet unbroken capsule cysts with up to three cells inside. This
is the first report of encystment induction of Azotobacter species grown on kerosene. 相似文献
10.