全文获取类型
收费全文 | 999篇 |
免费 | 10篇 |
国内免费 | 49篇 |
出版年
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 6篇 |
2015年 | 13篇 |
2014年 | 21篇 |
2013年 | 16篇 |
2012年 | 13篇 |
2011年 | 31篇 |
2010年 | 9篇 |
2009年 | 39篇 |
2008年 | 50篇 |
2007年 | 46篇 |
2006年 | 38篇 |
2005年 | 40篇 |
2004年 | 37篇 |
2003年 | 33篇 |
2002年 | 16篇 |
2001年 | 14篇 |
2000年 | 26篇 |
1999年 | 24篇 |
1998年 | 26篇 |
1997年 | 23篇 |
1996年 | 31篇 |
1995年 | 34篇 |
1994年 | 21篇 |
1993年 | 39篇 |
1992年 | 27篇 |
1991年 | 25篇 |
1990年 | 27篇 |
1989年 | 23篇 |
1988年 | 23篇 |
1987年 | 22篇 |
1986年 | 41篇 |
1985年 | 28篇 |
1984年 | 31篇 |
1983年 | 10篇 |
1982年 | 27篇 |
1981年 | 28篇 |
1980年 | 31篇 |
1979年 | 14篇 |
1978年 | 8篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1974年 | 1篇 |
排序方式: 共有1058条查询结果,搜索用时 140 毫秒
1.
2.
Electrophilic fatty acid nitroalkenes (NO2-FA) are products of nitric oxide and nitrite-mediated unsaturated fatty acid nitration. These electrophilic products induce pleiotropic signaling actions that modulate metabolic and inflammatory responses in cell and animal models. The metabolism of NO2-FA includes reduction of the vinyl nitro moiety by prostaglandin reductase-1, mitochondrial β-oxidation, and Michael addition with low molecular weight nucleophilic amino acids. Complex lipid reactions of fatty acid nitroalkenes are not well defined. Herein we report the detection and characterization of NO2-FA-containing triacylglycerides (NO2-FA-TAG) via mass spectrometry-based methods. In this regard, unsaturated fatty acids of dietary triacylglycerides are targets for nitration reactions during gastric acidification, where NO2-FA-TAG can be detected in rat plasma after oral administration of nitro-oleic acid (NO2-OA). Furthermore, the characterization and profiling of these species, including the generation of beta oxidation and dehydrogenation products, could be detected in NO2-OA-supplemented adipocytes. These data revealed that NO2-FA-TAG, formed by either the direct nitration of esterified unsaturated fatty acids or the incorporation of nitrated free fatty acids into triacylglycerides, contribute to the systemic distribution of these reactive electrophilic mediators and may serve as a depot for subsequent mobilization by lipases to in turn impact adipocyte homeostasis and tissue signaling events. 相似文献
3.
Fire can cause severe nitrogen (N) losses from grassland, chaparral, and temperate and boreal forest ecosystems. Paradoxically,
soil ammonium levels are markedly increased by fire, resulting in high rates of primary production in re-establishing plant
communities. In a manipulative experiment, we examined the influence of wild-fire ash residues on soil, microbial and plant
N pools in a recently burned Californian bishop pine (Pinus muricata D. Don) forest. Ash stimulated post-fire primary production and ecosystem N retention through direct N inputs from ash to
soils, as well as indirect ash effects on soil N availability to plants. These results suggest that redistribution of surface
ash after fire by wind or water may cause substantial heterogeneity in soil N availability to plants, and could be an important
mechanism contributing to vegetation patchiness in fire-prone ecosystems. In addition, we investigated the impact of fire
on ecosystem N cycling by comparing 15N natural abundance values from recently burned and nearby unburned P. muricata forest communities. At the burned site, 15N natural abundance in recolonising species was similar to that in bulk soil organic matter. By contrast, there was a marked
15N depletion in the same species relative to the total soil N pool at the unburned site. These results suggest that plant uptake
of nitrate (which tends to be strongly depleted in 15N because of fractionation during nitrification) is low in recently burned forest communities but could be an important component
of eco- system N cycling in mature conifer stands.
Received: 29 June 1999 / Accepted: 24 October 1999 相似文献
4.
5.
The two enzymes involved in the assimilatory pathway of nitrate in Azotobacter vinelandii are corregulated. Nitrate reductase and nitrite reductase are inducible by nitrate and nitrite. Ammonium represses induction by nitrate of both reductases. Repression by ammonium is higher in media containing 2-oxo-glutarate as carbon source than in media containing sucrose. Mutants in the gene ntrC lost nitrate and nitrite reductase simultaneously. Ten chlorate-resistant mutants with a new phenotype were isolated. In media without ammonium they had a normal phenotype, being sensitive to the toxic effect of chlorate. In media containing low ammonium concentrations they were resistant to chlorate. These mutants seem to be affected in the repression of nitrate and nitrite reductases by ammonium. 相似文献
6.
Summary The rate of flow and nitrate and phosphorus content of the water from four drained sandy and clayey plots of size 12×60 m
cropped to continuous corn were determined following two annual applications of different rates (0, 260, 390 and 520 kg N/ha)
of cow manure slurry. The drain flow was directly related to the rainfall and was greatly influenced by soil texture. The
N losses were greater in 1972 (7.8 to 19.1 kg N/ha) than in 1971 (0.4 to 7.8 kg N/ha) because of more summer rainfall. Nitrogen
and phosphorus losses were larger from the sandy plots than from the clayey plots. The manure application rates had no apparent
effect on nitrogen losses in the drain water. 相似文献
7.
K. Govindaraju M. Toporsian M. E. Ward D. K. Lloyd E. A. Cowley D. H. Eidelman 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,762(2)
We developed a simple capillary electrophoresis (CE) method to measure nitrite and nitrate concentrations in sub-microliter samples of rat airway surface liquid (ASL), a thin (10–30 μm) layer of liquid covering the epithelial cells lining the airways of the lung. The composition of ASL has been poorly defined, in large part because of the small sample volume (1–3 μl per cm2 of epithelium) and difficulty of harvesting ASL. We have used capillary tubes for ASL sample collection, with microanalysis by CE using a 50 mM phosphate buffer (pH 3), with 0.5 mM spermine as a dynamic flow modifier, and direct UV detection at 214 nm. The limit of detections (LODs), under conditions used, for ASL analysis were 10 μM for nitrate and 30 μM for nitrite (S/N=3). Nitrate and nitrite were also measured in rat plasma. The concentration of nitrate was 102±12 μM in rat ASL and 70±1.0 μM in rat plasma, whereas nitrite was 83±28 μM in rat ASL and below the LOD in rat plasma. After instilling lipopolysaccharide intratracheally to induce increased NO production, the nitrate concentration in ASL increased to 387±16 μM, and to 377±88 μM in plasma. The concentration of nitrite increased to 103±7.0 μM for ASL and 138±17 μM for plasma. 相似文献
8.
9.