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1.
Ductal epithelial cells of the exocrine pancreas secrete HCO3− rich, alkaline pancreatic juice, which maintains the intraluminal pH and washes the digestive enzymes out from the ductal system. Importantly, damage of this secretory process can lead to pancreatic diseases such as acute and chronic pancreatitis. Intracellular Ca2+ signaling plays a central role in the physiological regulation of HCO3− secretion, however uncontrolled Ca2+ release can lead to intracellular Ca2+ overload and toxicity, including mitochondrial damage and impaired ATP production. Recent findings suggest that the most common pathogenic factors leading to acute pancreatitis, such as bile acids, or ethanol and ethanol metabolites can evoke different types of intracellular Ca2+ signals, which can stimulate or inhibit ductal HCO3− secretion. Therefore, understanding the intracellular Ca2+ pathways and the mechanisms which can switch a good signal to a bad signal in pancreatic ductal epithelial cells are crucially important. This review summarizes the variety of Ca2+ signals both in physiological and pathophysiological aspects and highlight molecular targets which may strengthen our old friend or release our nasty enemy. 相似文献
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Protein secretion in streptomycetes 总被引:1,自引:0,他引:1
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David A. Dik Daniel R. Marous Jed F. Fisher 《Critical reviews in biochemistry and molecular biology》2017,52(5):503-542
The lytic transglycosylases (LTs) are bacterial enzymes that catalyze the non-hydrolytic cleavage of the peptidoglycan structures of the bacterial cell wall. They are not catalysts of glycan synthesis as might be surmised from their name. Notwithstanding the seemingly mundane reaction catalyzed by the LTs, their lytic reactions serve bacteria for a series of astonishingly diverse purposes. These purposes include cell-wall synthesis, remodeling, and degradation; for the detection of cell-wall-acting antibiotics; for the expression of the mechanism of cell-wall-acting antibiotics; for the insertion of secretion systems and flagellar assemblies into the cell wall; as a virulence mechanism during infection by certain Gram-negative bacteria; and in the sporulation and germination of Gram-positive spores. Significant advances in the mechanistic understanding of each of these processes have coincided with the successive discovery of new LTs structures. In this review, we provide a systematic perspective on what is known on the structure–function correlations for the LTs, while simultaneously identifying numerous opportunities for the future study of these enigmatic enzymes. 相似文献
5.
利用光镜及透射电子显微镜技术研究了杭白芷根中分泌道结构及其挥发油的分泌,并重点探讨分泌道中挥发油的分泌过程。结果显示:(1)杭白芷的分泌道是由上皮细胞围绕着的伸长的胞间隙,腔道内贮存着挥发油。(2)分泌道细胞的质体、细胞基质以及线粒体参与挥发油或其前体物质的合成。(3)在分泌道发育的后期,大量小泡与分泌细胞的液泡膜和细胞质膜融合,将其内的物质释放进入空腔。研究认为,杭白芷分泌道中挥发油主要合成部位为质体及细胞基质,之后以扩散渗透或通过膜质小泡与液泡及质膜融合这两种方式分泌到空腔内,丰富的线粒体可能为这一系列过程提供能量。 相似文献
6.
Gram‐negative bacteria cause many types of infections in animals from fish and shrimps to humans. Bacteria use Type III secretion systems (TTSSs) to translocate their toxins directly into eukaryotic cells. The V‐antigen is a multifunctional protein required for the TTSS in Yersinia and Pseudomonas aeruginosa. V‐antigen vaccines and anti‐V‐antigen antisera confer protection against Yersinia or P. aeruginosa infections in animal models. The V‐antigen forms a pentameric cap structure at the tip of the Type III secretory needle; this structure, which has evolved from the bacterial flagellar cap structure, is indispensable for toxin translocation. Various pathogenic gram‐negative bacteria such as Photorhabdus luminescens, Vibrio spp., and Aeromonas spp. encode homologs of the V‐antigen. Because the V‐antigens of pathogenic gram‐negative bacteria play a key role in toxin translocation, they are potential therapeutic targets for combatting bacterial virulence. In the USA and Europe, these vaccines and specific antibodies against V‐antigens are in clinical trials investigating the treatment of Yersinia or P. aeruginosa infections. Pathogenic gram‐negative bacteria are of great interest because of their ability to infect fish and shrimp farms, their potential for exploitation in biological terrorism attacks, and their ability to cause opportunistic infections in humans. Thus, elucidation of the roles of the V‐antigen in the TTSS and mechanisms by which these functions can be blocked is critical to facilitating the development of improved anti‐V‐antigen strategies. 相似文献
7.
Kristian Aspegren Leena Mannonen Anneli Ritala Riitta Puupponen-Pimiä Ulrika Kurtén Marjatta Salmenkallio-Marttila Veli Kauppinen Teemu H. Teeri 《Molecular breeding : new strategies in plant improvement》1995,1(1):91-99
Transgenic plant cell cultures have a potential for production and secretion of important proteins and peptides. To assess the possibilities of using a stable barley suspension culture for secretion of heterologous proteins in active form, we expressed the cDNA of the thermostable-glucanase (EGI) ofTrichoderma reesei in barley suspension cells. The cDNA coding for EGI and its signal sequence was placed under the control of the CaMV 35S promoter and the construction was transferred to the cells by particle bombardment. Stably transformed lines were obtained by selecting for a cotransformed antibiotic resistance marker. The expression of EGI cDNA led to accumulation of EGI in the culture medium, as shown by analysis with EGI-specific antibodies. Enzymatic assays confirmed that the EGI secreted by the suspension cells retained its activity and thermostable character. Furthermore, it was shown that the enzyme produced by the transgenic suspension culture could be used for degradation of soluble-glucans during mashing. 相似文献
8.
L-型细菌蛋白表达系统 总被引:2,自引:0,他引:2
L-型细胞由于缺乏细胞壁以及具有的其它特殊生物学特性,通过连接合适的信号肽,可以用于许多外源蛋白的可溶性,功能活性形式的分泌表达,表达产物在培养基中,表达的产量依赖于不同的基因序列,载体,宿主和诱导表达条件等因素,此外,L-型细菌还能将具有功能活性的蛋白展示在胞浆膜表面。 相似文献
9.
耐铝的和对铝敏感的玉米自交系根系的有机酸分泌 总被引:21,自引:3,他引:18
对玉米不同耐铝自交系在含A1^3 (0.1mmol/L)的完全营养液和A1C13 14.3μmol/L CaCl2 227.5μmol/L溶液等两种溶液中根系有机酸的分泌特征进行了研究。铝胁迫下,耐铝自交系Z1的生长与正常偏离不大,表现出较强的耐铝性,而铝敏感自交系Z2的生长则受到明显抑制。2种自交系根系分泌的有机酸种类包括苹果酸、柠檬酸、琥珀酸、马米酸、乙酸和草酸等,以苹果酸为主;其分泌量随铝处理时间而异。在两种溶液中,铝胁迫均可显著增加Z1苹果酸分泌量,且根系内苹果酸含量也显著增加。铝胁迫下,Z1根系NADP—苹果酸脱氢酶活性显著增加。从对试验结果分析得出:根系分泌苹果酸可能是玉米耐铝自交系适应酸性土壤逆境的生理特性之一,而分泌的苹果酸可能是在根系中通过PEP→4OAA→苹果酸途径合成的。 相似文献
10.
TFG clusters COPII‐coated transport carriers and promotes early secretory pathway organization 下载免费PDF全文
Adam Johnson Nilakshee Bhattacharya Michael Hanna Janice G Pennington Amber L Schuh Lei Wang Marisa S Otegui Scott M Stagg Anjon Audhya 《The EMBO journal》2015,34(6):811-827
In mammalian cells, cargo‐laden secretory vesicles leave the endoplasmic reticulum (ER) en route to ER‐Golgi intermediate compartments (ERGIC) in a manner dependent on the COPII coat complex. We report here that COPII‐coated transport carriers traverse a submicron, TFG (Trk‐fused gene)‐enriched zone at the ER/ERGIC interface. The architecture of TFG complexes as determined by three‐dimensional electron microscopy reveals the formation of flexible, octameric cup‐like structures, which are able to self‐associate to generate larger polymers in vitro. In cells, loss of TFG function dramatically slows protein export from the ER and results in the accumulation of COPII‐coated carriers throughout the cytoplasm. Additionally, the tight association between ER and ERGIC membranes is lost in the absence of TFG. We propose that TFG functions at the ER/ERGIC interface to locally concentrate COPII‐coated transport carriers and link exit sites on the ER to ERGIC membranes. Our findings provide a new mechanism by which COPII‐coated carriers are retained near their site of formation to facilitate rapid fusion with neighboring ERGIC membranes upon uncoating, thereby promoting interorganellar cargo transport. 相似文献