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川一阶导数光谱法测定猫豆中主旋多巴的含量,可以排除无关杂质吸收的干扰,省去复杂的分离提取步骤。试验结果表明:相关系数达0.9999,平均回收率101.5%,重现性较好。 相似文献
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Di Patrizi L Rosati F Guerranti R Pagani R Gerwig GJ Kamerling JP 《Glycoconjugate journal》2006,23(7-8):599-609
Mucuna pruriens seeds are used in some countries as a human prophylactic oral anti-snake remedy. Aqueous extracts of M. pruriens seeds possess in vivo activity against cobra and viper venoms, and protect mice against Echis carinatus venom. It was recently demonstrated that the seed immunogen generating the antibody that cross-reacts with the venom proteins
is a multiform glycoprotein (gpMuc), and the immunogenic properties of gpMuc seemed to mainly reside in its glycan chains.
In the present study, gpMuc was found to contain only N-glycans. Part of the N-glycans could be released with peptide-(N
4-(N-acetyl-β -glucosaminyl)asparagine amidase F (PNGase F-sensitive N-glycans); the PNGase F-resistant N-glycans were PNGase
A-sensitive. The oligosaccharides released were analyzed by a combination of MALDI-TOF mass spectrometry, HPLC profiling of
2-aminobenzamide-labelled derivatives and 1H NMR spectroscopy. The PNGase F-sensitive N-glycans comprised a mixture of oligomannose-type structures ranging from Man5GlcNAc2 to Man9GlcNAc2, and two xylosylated structures, Xyl1Man3GlcNAc2 and Xyl1Man4GlcNAc2. The PNGase A-sensitive N-glycans, containing (α 1-3)-linked fucose, were identified as Fuc1Xyl1Man2GlcNAc2 and Fuc1Xyl1Man3GlcNAc2. In view of the determined N-glycan ensemble, the immunoreactivity of gpMuc was ascribed to the presence of core (β 1-2)-linked
xylose- and core α (1-3)-linked fucose-modified N-glycan chains. 相似文献
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An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants
Summary The purpose of this study was to develop an efficient micropropagation system for Mucuna pruriens, an important medicinal plant in India. A range of cytokinins was investigated for multiple shoot regeneration with cotyledonary
node explants from 7-d-old aseptic seedlings. Of all the cytokinins, 6-benzyladenine (BA), kinetin (KIN) and 2-isopentenyl
adenine (2-iP) tested in Murashige and Skoog medium (MS), BA was the most effective and 5.0 μM was found to be optimum for inducing maximum shoots. Medium types, medium strength and pH were also investigated for induction
and proliferation of shoots. The highest efficiency of shoot proliferation was observed in 5.0 μM BA and 0.5 μM α-naphthalene acetic acid (NAA) in half-strength MS medium at pH 5.8. The best condition for rooting was half-strength MS
medium solidified with agar and with 2.0 μM indole-3-butyric acid (IBA). After rooting, the plantlets were transferred to plastic pots filled with sterile soilrite where
90% grew and all exhibited normal development. 相似文献
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Cytokinin-like effects of pure caffeine were tested in bioassays specific for this hormonal activity [radish cotyledon growth
and chlorophyll (Chl) biosynthesis in cucumber cotyledon and tobacco cell suspension] and in cell elongation bioassays [elongation
of segments from soybean internode and internode elongation in dwarf cultivars of guandu (Cajanus cajan) and mucuna (Mucuna
deeringiana)]. 6-Benzyl-aminopurine and kinetin (KIN) were used for comparison with caffeine. Although weaker than those given
by cytokinins, positive responses were observed in all specific bioassays and in elongation of soybean internodes. A remarkable
synergistic effect between caffeine and KIN was observed for the synthesis of Chl in the tobacco cell suspension bioassay,
in which different concentrations of the alkaloid were combined with a single concentration of KIN. The hormone-like effect
of caffeine might be related to the resemblance between caffeine and adenine derivatives.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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The health-relevant functionality of Mucuna pruriens was improved by priming the seeds with elicitors of the pentose phosphate pathway (PPP) such as fish protein hydrolysates (FPHs), lactoferrin (LF) and oregano extract (OE) followed by dark germination. FPH elicited the highest phenolic content of 19 mg/g FW on day 1, which was 38% higher than control sprouts. OE enhanced Parkinson’s disease-relevant L-DOPA content by 33% on day 1 compared to control sprouts. Anti-diabetes-relevant α-amylase inhibition percent (AIP) and α-glucosidase inhibition percent (GIP) were high in the cotyledons and decreased following elicitation and sprouting. For potential anti-diabetic applications, low AIP and high GIP with moderate L-DOPA content on day 4 of dark germination could be optimal. Improved L-DOPA concentrations in a soluble phenolic and antioxidant-rich M. pruriens background on day 1 sprouts have potential for Parkinson’s disease management. 相似文献
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L. J. A. Capo-chichi D. B. Weaver C. M. Morton 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(8):1180-1188
Velvetbean (Mucuna sp.) is a self-pollinated crop classified within the Leguminosae. Using AFLP markers, gene diversity and phenetic relationships
were estimated in a collection of 40 velvetbean accessions from cultivated species and different eco-geographic regions. Eleven
selective primer combinations generated a total of 508 amplification products. The average number of scorable fragments was
23 per primer combination. A total of 251 polymorphic markers was detected. The polymorphisms obtained ranged from 36% to
61% with an average of 49%. The final phenetic trees were constructed using Nei and Li’s coefficient of similarity with UPGMA.
Other clustering algorithms were examined and all had high co-phenetic correlations, indicating the goodness of fit for the
resulting phylogenetic trees. The phenetic tree as well as principal component analysis (PCA) separated the 40 velvetbean
accessions into two main clusters. Bootstrap and Jackknife analyses were completed and their values indicated strong to moderate
support for the two main clusters. This grouping confirmed the existing phenological difference with regard to maturity. The
high values of the similarity coefficients observed (0.87 to 0.97) imply that the accessions used in this study are similar.
The level of genetic variability detected within the velvetbean accessions with AFLP analysis suggests that it is a reliable,
efficient, and effective marker technology for determining genetic relationships in velvetbean.
Received: 19 June 2000 / Accepted: 1 March 2001 相似文献
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Niesko Pras Giovanni E. Booi Durk Dijkstra Alan S. Horn Theo M. Malingré 《Plant Cell, Tissue and Organ Culture》1990,21(1):9-15
Although alginate-entrapped cells of Mucuna pruriens L. possess a low substrate specificity, only para-substituted monocyclic phenols have been ortho-hydroxylated into catechols so far. In this study, compounds with more complex chemical structures were found to be substrates using entrapped cells of M. pruriens as well as the partially purified Mucuna-phenoloxidase. Thus, 5-, 6- and 7-hydroxylated 2-aminotetralins and a tricyclic compound, 9-hydroxy N-n-propyl hexahydronaphthoxazine, were converted into catechols. After isolation using preparative HPLC, the identity of the products was confirmed by MS. In general, for the entrapped cells and the enzyme preparation identical substrate specificities were found.This publication is dedicated to the memory of Prof. Alan S. Horn, Ph.D., who deceased at January 2, 1990 相似文献