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排序方式: 共有87条查询结果,搜索用时 17 毫秒
1.
用光镜和电镜观察羽叶薰衣草(Lavandula pinnata L.)雄性不育小孢子发育过程的细胞形态学特征.结果表明:羽叶薰衣草花药4枚,每枚花药通常具4个小孢子囊.花药壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四面体及十字交叉型.小孢子的发育过程可分为造孢细胞期、减数分裂时期、小孢子发育早期、小孢子发育晚期.未观察到二胞花粉期和成熟花粉期.羽叶薰衣草花粉败育主要发生在单核花粉时期,细胞内物质解体并逐渐消失变成空壳花粉或花粉皱缩变形成为各种畸形的败育花粉.在此之前小孢子的发育正常.羽叶薰衣草小孢子不育机制体现在绒毡层过早解体、四分体时期以后各细胞中线粒体结构不正常、胼胝质壁与小孢子母细胞脱离、花药壁细胞中淀粉出现时间异常等. 壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四面体及十字交叉型.小孢子的发育过程可分为造孢细胞期、减数分裂时期、小孢子发育早期、小孢子发育晚期.未观察到二胞花粉期和成熟花粉期.羽叶薰衣草花粉败育主要发生在单核花粉时期,细胞内物质解体并逐渐消失变成空壳花粉或花粉皱缩变形成为各种畸形的败育花粉.在此 前小孢子的发育正常.羽叶薰衣草小孢子不育机制体现在绒毡层过早解体、四分体时期以后各细胞中线粒体结构不正常、胼胝质壁与小孢子母细胞脱离、花药壁细胞中淀粉出现时间异常等. 壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四 相似文献
2.
Xiaoyu Zhang Hailang Tong Zhiqiang Han Long Huang Jing Tian Zhixing Fu Yunyi Wu Ting Wang Deyi Yuan 《Physiology and Molecular Biology of Plants》2021,27(5):959
Camellia oleifera is believed to exhibit a complex intraspecific polyploidy phenomenon. Abnormal microsporogenesis can promote the formation of unreduced gametes in plants and lead to sexual polyploidy, so it is hypothesized that improper meiosis probably results in the formation of natural polyploidy in Camellia oleifera. In this study, based on the cytological observation of meiosis in pollen mother cells (PMCs), we found natural 2n pollen for the first time in Camellia oleifera, which may lead to the formation of natural polyploids by sexual polyploidization. Additionally, abnormal cytological behaviour during meiosis, including univalent chromosomes, extraequatorial chromosomes, early segregation, laggard chromosomes, chromosome stickiness, asynchronous meiosis and deviant cytokinesis (monad, dyads, triads), was observed, which could be the cause of 2n pollen formation. Moreover, we confirmed a relationship among the length–width ratio of flower buds, stylet length and microsporogenesis. This result suggested that we can immediately determine the microsporogenesis stages by phenotypic characteristics, which may be applicable to breeding advanced germplasm in Camellia oleifera.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12298-021-01002-5. 相似文献
3.
4.
Growth and development of conifer pollen tubes 总被引:1,自引:0,他引:1
Conifer pollen tubes are an important but underused experimental system in plant biology. They represent a major evolutionary
step in male gametophyte development as an intermediate form between the haustorial pollen tubes of cycads and Ginkgo and the structurally reduced and faster growing pollen tubes of flowering plants. Conifer pollen grains are available in
large quantities, most can be stored for several years, and they grow very well in culture. The study of pollen tube growth
and development furthers our understanding of conifer reproduction and contributes towards our ability to improve on their
productivity. This review covers taxonomy and morphology to cell, developmental, and molecular biology. It explores recent
advances in research on conifer pollen and pollen tubes in vivo, focusing on pollen wall structure, male gametophyte development
within the pollen wall, pollination mechanisms, pollen tube growth and development, and programmed cell death. It also explores
recent research in vitro, including the cellular mechanisms underlying pollen tube elongation, in vitro fertilization, genetic
transformation and gene expression, and pine pollen tube proteomics. With the ongoing sequencing of the Pinus taeda genome in several labs, we expect the use of conifer pollen tubes as an experimental system to increase in the next decade. 相似文献
5.
外来入侵种--紫茎泽兰花芽分化和胚胎学研究 总被引:5,自引:0,他引:5
应用石蜡切片法对紫茎泽兰(Eupatorium adenophorum)从花芽分化到胚胎形成的全过程进行系统观察,结果表明:紫茎泽兰从刚孕花序至头状花序的直径(d)大小为1.6~1.8 mm时为花芽分化期;d在1.8~2.0和3.8~4.0 mm范围内为大小孢子及雌雄配子体发育期,其中d在2.3~3.5 mm之间时为减数分裂的主要过程;药壁分化过程为双子叶型,胞质分裂为同时型,绒毡层发育属腺质绒毡层,成熟花粉粒为3-细胞型;胚囊发育属蓼形,胚珠倒生,单珠被,薄珠心;胚胎发育过程中有双胚现象. 相似文献
6.
7.
Background and Aims
In flowering plants, microsporogenesis is accompanied by various types of cytoplasmic partitioning (cytokinesis). Patterns of male cytokinesis are suspected to play a role in the diversity of aperture patterns found in pollen grains of angiosperms. The relationships between intersporal wall formation, tetrad shape and pollen aperture pattern ontogeny are studied.Methods
A comparative analysis of meiosis and aperture distribution was performed within tetrads in two triporate eudicot species with contrasting aperture arrangements within their tetrads [Epilobium roseum (Onagraceae) and Paranomus reflexus (Proteaceae)].Key Results and Conclusions
Intersporal wall formation is a two-step process in both species. Cytokinesis is first achieved by the formation of naked centripetal cell plates. These naked cell plates are then covered by additional thick, localized callose deposits that differ in location between the two species. Apertures are finally formed in areas in which additional callose is deposited on the cell plates. The recorded variation in tetrad shape is correlated with variations in aperture pattern, demonstrating the role of cell partitioning in aperture pattern ontogeny. 相似文献8.
Summary. A study of male sterility over a period of three consecutive years on a conifer species endemic to Taiwan, Taiwania cryptomerioides Hayata (Taxodiaceae), was done for this article. With the aids of fluorescence and electron microscopic observations, the
ontogenic processes in the fertile and sterile microsporangia are compared, using samples collected from Chitou Experimental
Forest and Yeou-Shoei-Keng Clonal Orchard of the National Taiwan University, Nantou, Taiwan. The development of male strobili
occurred from August to the end of March. Microsporogenesis starts with the formation of the archesporium and ends with the
maturation of 2-celled pollen grains within the dehiscing microsporangium. Before meiosis, there was no significant difference
in ultrastructure between the fertile and sterile microsporangia. Asynchronous pollen development with various tetrad forms
may occur in the same microsporangium of either fertile or sterile strobili. However, a callose wall was observable in the
fertile dyad and tetrad, but not in the sterile one. After dissolution of the callose wall, the fertile microspores were released
into the locule, while some sterile microspores still retained as tetrads or dyads with intertwining of exine walls in the
proximal faces. As a result, there was no well developed lamellated endexine and no granulate ectexine or intine in the sterile
microspores. Eventually, the intracellular structures in sterile microspores were dramatically collapsed before anthesis.
The present study shows that the abortion in pollen development is possibly attributed to the absence of the callose wall.
The importance of this structure to the male sterility of T. cryptomerioides is discussed.
Correspondence and reprints: Department of Life Science, National Taiwan University, 106 Taipei, Taiwan. 相似文献
9.
C. Conicella G. Genualdo R. Lucia K. S. Ramulu T. Cardi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):609-617
Somatic hybridization between Solanum commersonii and S. tuberosum resulted in the production of male-sterile hybrid plants, except for one fully male-fertile hybrid. The male-sterile hybrids
exhibited a“pollen-less” phenotype, with rare pollen grains which were abnormal in shape and exine sculpture. Microsporogenesis
and tapetal development were investigated both in male-sterile and male-fertile somatic hybrids to assess the cytological
events that were involved in male sterility. The pattern of male sterility was complex, arising through mechanisms expressed
at both sporophytic and gametophytic levels. Various abnormalities occurred first in the tapetum, and later during meiosis-II
and cytokinesis. These caused the degeneration of the sporads and of the microspores when they were released. In the male-fertile
hybrid, normal development of the tapetum and pollen mother cells was restored. The hypothesis that tapetal breakdown, meiosis-II
and cytokinesis defects are related to each other, and depend on nuclear-mitochondrial interactions, is discussed. Because
of the formation of multivalent chromosome configurations, it is likely that gene exchange between S. commersonii and S. tuberosum can occur in somatic hybrids, offering potential perspectives for the introgression of useful traits from S. commersonii into S. tuberosum.
Received: 10 December 1996/Accepted: 21 March 1997 相似文献
10.
Summary In the ogu cytoplasmic male-sterile (CMS) line of Brassica napus, stamen morphology was influenced by temperature conditions. Under a high temperature regime (27° C/23° C; day/ night) CMS stamens had a near-normal morphology, but microsporogenesis proceeded to a maximum of the microspore stage. However, compared to the normal stamens, the occurrence of sporopollenin-like deposits in the tapetum and deposition of exine on the microspores was sparse. Also, the tapetal cells of the CMS line were often highly vacuolate and failed to degenerate at the same stage as the normal. Ultrastructural changes in the mitochondrial matrix and cristae plus dilation of the endoplasmic reticulum, which occurred during development in sporogenous tissues of the normal line, were often lacking or mistimed in the mutant. Due to extensive variation, even between adjacent locules, the cytological differences between the normal and CMS anthers cannot be ascribed as the cause of male sterility in the ogu CMS line of B. napus, rather they may be the consequence of it. 相似文献