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1.
Identification and characterization of new plant microRNAs using EST analysis   总被引:46,自引:0,他引:46  
Seventy-five previously known plant microRNAs (miRNAs) were classified into 14 families according to their gene sequence identity. A total of 18,694 plant expressed sequence tags (EST) were found in the GenBank EST databases by comparing all previously known Arabidopsis miRNAs to GenBank‘s plant EST databases with BLAST algorithms. After removing the EST sequences with high numbers (more than 2) of mismatched nucleotides, a total of 812 EST contigs were identified. After predicting and scoring the RNA secondary structure of the 812 EST sequences using mFold software, 338 new potential miRNAs were identified in 60 plant species, miRNAs are widespread. Some microRNAsmay highly conserve in the plant kingdom, and they may have the same ancestor in very early evolution. There is no nucleotide substitution in most miRNAs among many plant species. Some of the new identified potential miRNAs may be induced and regulated by environmental biotic and abiotic stresses. Some may be preferentially expressed in specific tissues, and are regulated by developmental switching. These findings suggest that EST analysis is a good alternative strategy for identifying new miRNA candidates, their targets, and other genes. A large number of miRNAs exist in different plant species and play important roles in plant developmental switching and plant responses to environmental abiotic and biotic stresses as well as signal transduction. Environmental stresses and developmental switching may be the signals for synthesis and regulation of miRNAs in plants. A model for miRNA induction and expression, and gene regulation by miRNA is hypothesized.  相似文献
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Human embryonic stem cells express a unique set of microRNAs   总被引:41,自引:0,他引:41  
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MicroRNA biogenesis and function in plants   总被引:31,自引:0,他引:31  
Chen X 《FEBS letters》2005,579(26):5923-5931
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Molecular evolution of a microRNA cluster   总被引:27,自引:0,他引:27  
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MicroRNA function in animal development   总被引:27,自引:0,他引:27  
Wienholds E  Plasterk RH 《FEBS letters》2005,579(26):5911-5922
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Dicing and slicing: the core machinery of the RNA interference pathway   总被引:26,自引:0,他引:26  
Hammond SM 《FEBS letters》2005,579(26):5822-5829
RNA interference (RNAi) is broadly defined as a gene silencing pathway that is triggered by double-stranded RNA (dsRNA). Many variations have been described on this theme. The dsRNA trigger can be supplied exogenously, as an experimental tool, or can derive from the genome in the form of microRNAs. Gene silencing can be the result of nucleolytic degradation of the mRNA, or by translational suppression. At the heart of the pathway are two ribonuclease machines. The ribonuclease III enzyme Dicer initiates the RNAi pathway by generating the active short interfering RNA trigger. Silencing is effected by the RNA-induced silencing complex and its RNaseH core enzyme Argonaute. This review describes the discovery of these machines and discusses future lines of work on this amazing biochemical pathway.  相似文献
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Identification and characterization of small RNAs involved in RNA silencing   总被引:22,自引:0,他引:22  
Aravin A  Tuschl T 《FEBS letters》2005,579(26):5830-5840
Double-stranded RNA (dsRNA) is a potent trigger of sequence-specific gene silencing mechanisms known as RNA silencing or RNA interference. The recognition of the target sequences is mediated by ribonucleoprotein complexes that contain 21- to 28-nucleotide (nt) guide RNAs derived from processing of the trigger dsRNA. Here, we review the experimental and bioinformatic approaches that were used to identify and characterize these small RNAs isolated from cells and tissues. The identification and characterization of small RNAs and their expression patterns is important for elucidating gene regulatory networks.  相似文献
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Plant microRNA: a small regulatory molecule with big impact   总被引:18,自引:0,他引:18  
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