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1.
Coronary Artery Disease (CAD) is a major killer disease throughout the world. Dyslipidemia is a major contributor to the risk of CAD. Several dietary articles traditionally used in India and other South Asian countries reduced dyslipidemia. The present study was undertaken to evaluate the combined effect of Mung bean (Vigna radiata) and Elephant foot yam (Amorphophallus paeoniifolius) on serum lipids and atherogenic indices in albino rats and to compare it with a standard drug Cholestyramine. Thirty healthy albino rats of both sexes (150–200 g) were randomized to 5 groups of 6 animals each. The grouping were done based on the following criteria: Group I: Normal Control Group, Group II: (Standard Group): Cholestyramine resin 5 mg/kg bw, Group III: (Half Dose Group): Drug powder at 540 mg/kg bw, Group IV: (Effective Dose Group): Drug powder at 1080 mg/kg bw, and Group V: (Double Dose Group): Drug powder at 2160 mg/kg bw. Lipid profile was estimated at the beginning and after 30 days of treatment. The Effective and Double doses of the drug reduced Total cholesterol along with levels of Triglycerides, Low density lipoprotein and Very low density lipoprotein levels significantly (p < 0.01) along with a significant (p < 0.01) increase in high density lipoproteins (HDL) in rats. There was also significant (p < 0.01) improvement in atherogenic indices like Castelli Risk Index I, Non HDL C/HDL, Castelli risk Index II, TG/HDL, Atherogenic coefficient and Atherogenic Index of Plasma. The combination of powdered sprouted mung bean and yam powder have excellent lipid lowering potential.  相似文献   
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Unique species of ceramide (Cer) with very-long-chain polyunsaturated fatty acid (VLCPUFA), mainly 28–32 carbon atoms, 4–5 double bonds, in nonhydroxy and 2-hydroxy forms (n-V Cer and h-V Cer, respectively), are generated in rat spermatozoa from the corresponding sphingomyelins during the acrosomal reaction. The aim of this study was to determine the properties of these sperm-distinctive ceramides in Langmuir monolayers. Individual Cer species were isolated by HPLC and subjected to analysis of surface pressure, surface potential, and Brewster angle microscopy (BAM) as a function of molecular packing. In comparison with known species of Cer, n-V Cer and h-V Cer species showed much larger mean molecular areas and increased molecular dipole moments in liquid expanded phases, which suggest bending and partial hydration of the double bonded portion of the VLCPUFA. The presence of the 2-hydoxyl group induced a closer molecular packing in h-V Cer than in their chain-matched n-V Cer. In addition, all these Cer species showed liquid-expanded to liquid-condensed transitions at room temperature. Existence of domain segregation was confirmed by BAM. Additionally, thermodynamic analysis suggests a phase transition close to the physiological temperature for VLCPUFA-Cers if organized as bulk dispersions.  相似文献   
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Sticholysin I (St I) is a pore-forming toxin (PFT) produced by the Caribbean Sea anemone Stichodactyla helianthus belonging to the actinoporin protein family, a unique class of eukaryotic PFT exclusively found in sea anemones. As for actinoporins, it has been proposed that the presence of sphingomyelin (SM) and the coexistence of lipid phases increase binding to the target membrane. However, little is known about the role of membrane structure and dynamics (phase state, fluidity, presence of lipid domains) on actinoporins' activity or which regions of the membrane are the most favorable platforms for protein insertion. To gain insight into the role of SM on the interaction of St I to lipid membranes we studied their binding to monolayers of phosphatidylcholine (PC) and SM in different proportions. Additionally, the effect of acyl chain length and unsaturation, two features related to membrane fluidity, was evaluated on St I binding to monolayers. This study revealed that St I binds and penetrates preferentially and with a faster kinetic to liquid-expanded films with high lateral mobility and moderately enriched in SM. A high content of SM induces a lower lateral diffusion and/or liquid-condensed phases, which hinder St I binding and penetration to the lipid monolayer. Furthermore, the presence of lipid domain borders does not appear as an important factor for St I binding to the lipid monolayer.  相似文献   
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目的:检测Myc和TP53在食管癌组织和远端无癌组织的表达情况,并分析其与临床病理因素之间的关系,初步探讨Myc和TP53在新疆食管癌发生发展中可能存在的特点。方法:经Trizol一步法提取88例新疆地区食管癌组织及其远端无癌组织标本总RNA,m RNA逆转录为c DNA,经聚合酶链式反应生成产物,运用光密度值即半定量RT-PCR技术检测88例新疆地区食管癌组织、远端无癌组织中Myc和TP53的m RNA表达情况及二者阳性表达率,并分析Myc和TP53的表达与临床病理因素之间的相关性。结果:1 Myc的m RNA相对表达量在食管癌组织中高于远端无癌组织,差异有显著性(P0.01);TP53的m RNA相对表达量在远端无癌组织中高于癌组织(P0.05);2 Myc的阳性表达率在88例食管癌组织中高于远端无癌组织(P0.05);TP53的阳性表达率在远端无癌组织中高于癌组织(P0.05);3Myc的表达与分化程度(P0.01)、TNM分期(P0.01)、淋巴结转移(P0.05)、侵犯深度(P0.05)和族别(P0.05)有关,与性别无关;TP53的表达与侵犯程度(P0.01)有关,与性别、分化程度、TNM分期、淋巴结转移和族别均无关;4 88例食管癌组织中,Myc和TP53的表达呈现负相关(r=-0.501,P0.0 1)。结论:Myc在新疆地区食管癌组织中表达上调,TP53则表达减弱。说明Myc参与食管癌的发生和发展,而TP53则可能保护正常组织不发生癌变。  相似文献   
6.
Bar-built estuaries are generally found at the mouths of smaller watersheds with seasonal precipitation, episodic streamflow and seasonal swell dynamics. Low streamflows and constructive wave forces form a sand bar at the mouth isolating the stream from the ocean, creating a ponded lagoon, and inundating the surrounding marsh plain. Bar-built estuaries are wide spread in California comprising over 50 percent of California's more than 500 estuaries. By connecting terrestrial, freshwater, and marine realms bar-built estuaries are complex and dynamic providing great habitat and ecosystem services. California has suffered some of the highest losses of wetland habitats, and the wetland habitats of bar-built estuaries continue to be threatened by further development, pollution, and climate related changes including diminished streamflows and sea level rise. Given this loss and threat we developed a California Rapid Assessment Method (CRAM) to assess the condition of California's bar-built estuaries. CRAM uses visual indicators to accurately reflect current wetland condition with regards to buffer habitat, hydrology, physical complexity, plant diversity and structure, and landscape influences. Here we validate this method by comparing results of CRAM for bar-built estuaries to other accepted measures of wetland condition that we simultaneously collected with CRAM including vegetative surveys, water nutrient levels, and GIS landscape scale measures of stress for 32 sites throughout California. CRAM correlated well with each of these three independent methods of assessing condition. Notably, the Environmental Monitoring and Assessment Program (EMAP) number of natives metric significantly correlated with CRAM Index and all Attribute scores. The strong correlations of CRAM to nutrient levels is particularly important considering the documentation of the negative impact of nutrients on fish populations, the use of bar-built estuaries by juveniles of commercially important species, and the nursery role of bar-built estuaries for maintaining imperiled populations of species such as steelhead. GIS measured percent impervious, percent agriculture, and percent dams all correlated well with expected CRAM Attribute scores at appropriate watershed scales relative to the area of inference for each CRAM metric. Further, CRAM for bar-built estuaries works well throughout California's diversity of environmental conditions and regardless of geography, timing, or whether the bar was open or closed during the survey. We hope that the availability of CRAM combined with available data repositories will enable local, state, and federal decision makers to better manage, restore, and conserve valuable bar-built estuaries in the face of continual threats like development, drought, and sea level rise.  相似文献   
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以贵州西南部喀斯特山区35年生和14年生花椒林为研究对象,并以未退耕旱地为对照,研究表层土壤(0~20 cm)水稳性团聚体分布及有机碳矿化特征,探讨土壤有机碳周转对不同花椒种植年限的响应。结果表明:随团聚体粒径的降低,两种年龄花椒林土壤水稳性团聚体含量呈现先增加后减少的倒"V"形分布,土壤水稳性团聚体分布主要出现在5~2、2~1和1~0.5 mm 3个粒级中;与旱地相比,花椒种植明显增加了全土和团聚体中有机碳和活性有机碳含量,并以14年生花椒林较高,而35年生花椒林存在较明显的衰减;随花椒种植年限的增加,土壤有机碳矿化累积量呈递减趋势,但土壤有机碳周转半衰期以14年生花椒林较长,显著高于旱地和35年生花椒林,表明14年生花椒林土壤有机碳更易累积,表现出较好的土壤碳固存能力;喀斯特山区种植花椒后,土壤有机碳存在"汇-源"的转换过程,因此花椒种植应注重长期维护管理,防止土壤质量的衰退。  相似文献   
9.
目的:探讨脑电图检查对新生儿缺氧缺血性脑病早期诊断及预后判断的临床意价值。方法:本研究所选研究对象为我院2011年3月至2014年3月住院的91例出生后一天内的窒息新生儿,对其行脑电图检查,根据脑电图的检查结果将其分为四组,分别为正常组、轻度异常组、中度异常组、重度异常组。对上述四组患儿进行随访,时间点为出生后3个月、6个月,采用婴幼儿发育量表对患儿的智能发育进行测评,对各组智能发育的差异进行比较。结果:(1)91例患儿中,脑电图异常的有83例,异常率为91.21%,脑电图的异常程度与临床分度基本一致;(2)对患儿随访至3个月时,轻度、中度、重度异常组患儿的智力发育指数与运动发育指数的平均值与正常组患儿相比,具有显著差异(P0.05);(3)对患儿随访至6个月时,轻度异常组患儿的智力发育指数与运动发育指数的平均值与正常组患儿相比,无显著差异(P0.05)。中度、重度异常组患儿的智力发育指数与运动发育指数的平均值与正常组患儿相比,具有显著差异(P0.05)。结论:早期脑电图检查结果对新生儿缺氧缺血性脑病的早期诊断有利,为临床上早期干预提供了重要的参考依据。  相似文献   
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