首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   306篇
  国内免费   9篇
  完全免费   64篇
  2019年   7篇
  2018年   31篇
  2017年   18篇
  2016年   8篇
  2015年   8篇
  2014年   36篇
  2013年   14篇
  2012年   22篇
  2011年   23篇
  2010年   7篇
  2009年   14篇
  2008年   27篇
  2007年   28篇
  2006年   21篇
  2005年   17篇
  2004年   10篇
  2003年   9篇
  2002年   12篇
  2001年   8篇
  2000年   9篇
  1999年   2篇
  1998年   4篇
  1997年   10篇
  1996年   6篇
  1995年   5篇
  1993年   3篇
  1992年   3篇
  1991年   2篇
  1990年   1篇
  1989年   4篇
  1988年   1篇
  1987年   1篇
  1985年   2篇
  1983年   2篇
  1980年   1篇
  1977年   1篇
  1972年   1篇
  1971年   1篇
排序方式: 共有379条查询结果,搜索用时 46 毫秒
1.
2.
We previously reported a new in vivo model named as "GFP/CCl(4) model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl(4) induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver.  相似文献
3.
Hepatic fibrosis is a scarring process that is associated with an increased and altered deposition of extracellular matrix in liver. At the cellular and molecular level, this progressive process is mainly characterized by cellular activation of hepatic stellate cells and aberrant activity of transforming growth factor-beta1 and its downstream cellular mediators. Although the cellular responses to this cytokine are complex, the signalling pathways of this pivotal cytokine during the fibrogenic response and its connection to other signal cascades are now understood in some detail. Based on the current advances in understanding the pleiotropic reactions during fibrogenesis, various inhibitors of transforming growth factor-beta were developed and are now being investigated as potential drug candidates in experimental models of hepatic injury. Although it is too early to favour one of these antagonists for the treatment of hepatic fibrogenesis in human, the experimental results obtained yet provide stimulatory impulses for the development of an effective treatment of choice in the not too distant future. The present review summarises the actual knowledge on the pathogenesis of hepatic fibrogenesis, the role of transforming growth factor-beta and its signalling pathways in promoting the fibrogenic response, and the therapeutic modalities that are presently in the spotlight of many investigations and are already on the way to take the plunge into clinical studies.  相似文献
4.
放线菌分离方法的研究Ⅰ.抑制剂的选择   总被引:19,自引:0,他引:19       下载免费PDF全文
抑制剂的选择是放线菌分离工作必须解决的一个重要问题。研究了重铬酸钾对放线菌、细菌、真菌的作用。结果表明,它对土壤真菌、细菌有明显的抑制作用,而对放线菌无抑制作用,可作为选择性分离放线菌的一种高效、便宜的抑制剂。本文还对HV培养基进行了改良。  相似文献
5.
短间隔连续部分肝切除对大鼠生存和肝组织结构的影响   总被引:17,自引:0,他引:17  
徐存拴  李永辉  段瑞峰  卢爱灵  夏民  吉爱玲 《动物学报》2001,47(6):659-665,T001
在部分肝切除(partial hepatectomy,PH)后的细胞激活(G0-G1)期(4h)、有丝分裂高峰期(36h)及以两者交叉方式进行连续部分肝切除(successive partial hepatectomy,SPH),观察其对大鼠生存和肝组织结构的影响。结果表明,大鼠对短间隔(间隔4和/或36h)连续部分肝切除的耐受极限取决于各次切除的肝量和间隔时间两个因素;连续部分肝切除引起的肝组织结构紊乱程度与部分肝切除次数正相关;细胞核数、有丝分裂指数与短间隔连续部分肝切除次数和方式显现复杂的相关性。依SPH中大鼠成活率、肝组织结构变化、生理生化变化为依据,确立了4组(E、G、K和M组)适合研究肝再生分子机理的短间隔连续部分肝切除模型(short interval successive partial hepatectomy,SISPH)。  相似文献
6.
Integral role of the EGF receptor in HGF-mediated hepatocyte proliferation.   总被引:16,自引:0,他引:16  
Hepatocyte growth factor (HGF), insulin, and TGF-alpha stimulate DNA synthesis in cultured hepatocytes. Each ligand activates a distinct tyrosine kinase receptor, although receptor cross-talk modulates signaling. In rat hepatocytes, HGF can stimulate TGF-alpha production while TGF-alpha antibodies or antisense oligonucleotides suppress HGF-stimulated DNA synthesis. We report that the epidermal growth factor receptor (EGFR) kinase inhibitor PKI166 blocked both basal and ligand-induced tyrosine phosphorylation of the EGFR (IC(50) = 60 nM), but not of the insulin receptor or c-met. Pharmacologic inhibition of the EGFR kinase abolished the proliferative actions of HGF and EGF, but not insulin, whereas PI-3 kinase inhibition blocked both EGF and insulin actions. We conclude that in cultured hepatocytes (i) PI-3 kinase is required for EGF- and insulin-induced proliferation and (ii) EGFR mediates both the basal rate of DNA synthesis and that induced by EGF and HGF, but not insulin. The mitogenic effect of HGF may be secondary to increased synthesis or processing of EGFR ligands such as TGF-alpha.  相似文献
7.
8.
Biology and global distribution of myxobacteria in soils   总被引:14,自引:0,他引:14  
This review presents an overview of the present status of the biology of the myxobacteria, including the molecular biology of the systems that control and regulate myxobacterial gliding movement and morphogenesis. The present status of myxobacterial taxonomy and phylogeny is described. The evolutionary biology of the myxobacteria is emphasized with respect to their social behavior and the molecular basis of their signal chains. Most important within the metabolic physiology are the biologically active secondary metabolites of myxobacteria and their molecular mechanisms of action. The global distribution of myxobacteria in soils is described on the basis of data given in the literature as well as of comprehensive analyses of 1398 soil samples from 64 countries of all continents. The results are analyzed with respect to the spectrum and number of species depending on ecological and habitat-specific factors. The myxobacterial floras of different climate zones are compared. Included are myxobacterial species adapted to extreme biotopes. The efficiency of different methods used presently for isolation of myxobacteria is compared.  相似文献
9.
The mechanisms initiating and perpetuating the fibrogenic response in the injured liver are not well understood. Hepatic stellate cells are activated by liver injury to become proliferative and fibrogenic myofibroblasts. Emerging evidence suggests that the sympathetic nervous system may play a role in the development of cirrhosis. It is not known, however, whether this requires a direct interaction between sympathetic neurotransmitters and stellate cell receptors, or results indirectly, from sympathetic effects on the vasculature. Using cultured hepatic stellate cells, we show that the sympathetic neurotransmitters, norepinephrine and neuropeptide Y, markedly stimulate the proliferation of activated, myofibroblastic, hepatic stellate cells. Norepinephrine, but not neuropeptide Y, also induces collagen gene expression. In conclusion, physiologically relevant concentrations of sympathetic neurotransmitters directly modulate the phenotype of hepatic stellate cells. This suggests that targeted interruption of sympathetic nervous system signaling in hepatic stellate cells may be useful in constraining the fibrogenic response to liver injury.  相似文献
10.
肝再生增强因子研究进展   总被引:12,自引:0,他引:12  
肝再生增强因子是新近克隆的蛋白质因子,能特异地刺激肝源细胞的增殖,并对CCl4所引起的急性肝衰竭有效治作用。本文综述了肝再生增强因子的发现、基因克隆及组织分布等。目前已开始了该因子的基因工程产品研制,它有望成为一种治疗肝病的新药。  相似文献
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号