全文获取类型
收费全文 | 3052篇 |
免费 | 142篇 |
国内免费 | 189篇 |
出版年
2023年 | 51篇 |
2022年 | 62篇 |
2021年 | 78篇 |
2020年 | 69篇 |
2019年 | 117篇 |
2018年 | 95篇 |
2017年 | 90篇 |
2016年 | 68篇 |
2015年 | 132篇 |
2014年 | 204篇 |
2013年 | 200篇 |
2012年 | 139篇 |
2011年 | 122篇 |
2010年 | 93篇 |
2009年 | 137篇 |
2008年 | 130篇 |
2007年 | 151篇 |
2006年 | 151篇 |
2005年 | 133篇 |
2004年 | 142篇 |
2003年 | 108篇 |
2002年 | 85篇 |
2001年 | 70篇 |
2000年 | 69篇 |
1999年 | 87篇 |
1998年 | 71篇 |
1997年 | 36篇 |
1996年 | 45篇 |
1995年 | 47篇 |
1994年 | 49篇 |
1993年 | 27篇 |
1992年 | 46篇 |
1991年 | 51篇 |
1990年 | 28篇 |
1989年 | 19篇 |
1988年 | 24篇 |
1987年 | 22篇 |
1986年 | 23篇 |
1985年 | 18篇 |
1984年 | 20篇 |
1983年 | 7篇 |
1982年 | 15篇 |
1981年 | 11篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1978年 | 10篇 |
1977年 | 3篇 |
1976年 | 9篇 |
1974年 | 3篇 |
1973年 | 2篇 |
排序方式: 共有3383条查询结果,搜索用时 15 毫秒
1.
《DNA Repair》2017
Infliction of DNA damage initiates a complex cellular reaction – the DNA damage response – that involves both signaling and DNA repair networks with many redundancies and parallel pathways. Here, we reveal the three strategies that the simple multicellular eukaryote, C. elegans, uses to deal with DNA damage induced by light. Separately inactivating repair or replicative bypass of photo-lesions results in cellular hypersensitivity towards UV-light, but impeding repair of replication associated DNA breaks does not. Yet, we observe an unprecedented synergistic relationship when these pathways are inactivated in combination. C. elegans mutants that lack nucleotide excision repair (NER), translesion synthesis (TLS) and alternative end joining (altEJ) grow undisturbed in the dark, but become sterile when grown in light. Even exposure to very low levels of normal daylight impedes animal growth. We show that NER and TLS operate to suppress the formation of lethal DNA breaks that require polymerase theta-mediated end joining (TMEJ) for their repair. Our data testifies to the enormous genotoxicity of light and to the demand of multiple layers of protection against an environmental threat that is so common. 相似文献
2.
3.
A survey was made of the presence, copy number and location of the Salmonella-specific DNA insertion element IS200, within the genomes of the 27 phage type strains of Salmonella enteritidis. All the phage type strains contained copies of IS200 revealed by genomic Southern blot hybridizations with a 300-bp DNA probe internal to the element. Restriction site variation around IS200 insertion sites was examined. Three fundamental patterns of hybridization corresponding to chromosomal IS200 loci were found. In terms of population genetics, these 'IS200 profiles' correspond to clonal lineages of recent evolutionary origin, and underline the phage-typing scheme for epidemiological subdivision of S. enteritidis. The molecular analysis is consistent with genetic selection pressures which are apparent in the observed epidemiological distribution of S. enteritidis, since each clonal lineage contained one of the phage types of major clinical importance in the U.K. 相似文献
4.
E.M. del Campo A. del Hoyo C. Royo L.M. Casano R. lvarez E. Barreno 《Molecular phylogenetics and evolution》2010,57(3):1323-1328
5.
6.
7.
Sign language studies of cross-fostered chimpanzees measure the effect of special rearing conditions on the development of
very young chimpanzees. Cross-fostered chimpanzees, like human children, develop gradually in a process that takes many years.
Here we discuss details of the procedure, the overlap between human and chimpanzee infants in the contents of the first 50-item
vocabularies, and the ways in which the signs of the chimpanzees exhibit the fuzziness of natural language categories. We
also compare the cross-fostering approach with more traditional modular approaches to the study of language-like behavior
in nonhuman animals.
Project Washoe was originally supported by grants MH-12154 from the National Institute of Mental Health and GB-7432 from the
National Science Foundation. We gratefully acknowledge this support and the support that later sign language studies of chimpanzees
have received since then from NIH, NSF, the National Geographic Society, the Grant Foundation, the Spencer Foundation, the
University of Nevada, and the UNR Foundation. 相似文献
8.
9.
Adaptation of the tetrazolium method for testing the seed viability, and scanning electron microscopy study of some Western European orchids 总被引:3,自引:0,他引:3
A modified tetrazolium method was formulated for use with seeds of Western European orchids. The sequence of treatments which gave the highest percentage of coloured (i.e. viable) embryos was: (1) pretreatment in a solution of 5% (w/v) Ca(OCl)2 + 1% (v/v) Tween-80, (2) soaking for 1 day in sterile water, (3) the classical tetrazolium test. The optimal duration of the pretreatment in Ca(OCl)2 + Tween-80 depends upon the species, and to investigate the effect a scanning electron microscopy study was performed on the testa of 3 species. For a given species, the optimal pretreatment period was not affected by the year of harvest or the source of the seed lots. 相似文献
10.
Debating Eukaryogenesis—Part 2: How Anachronistic Reasoning Can Lure Us into Inventing Intermediates
Dave Speijer 《BioEssays : news and reviews in molecular, cellular and developmental biology》2020,42(5):1900153
Eukaryotic origins are inextricably linked with the arrival of a pre-mitochondrion of alphaproteobacterial-like ancestry. However, the nature of the “host” cell and the mode of entry are subject to heavy debate. It is becoming clear that the mutual adaptation of a relatively simple, archaeal host and the endosymbiont has been the defining influence at the beginning of the eukaryotic lineage; however, many still resist such symbiogenic models. In part 1, it is posited that a symbiotic stage before uptake (“pre-symbiosis”) seems essential to allow further metabolic integration of the two partners ending in endosymbiosis. Thus, the author argued against phagocytic mechanisms (in which the bacterium is prey or parasite) as the mode of entry. Such positions are still broadly unpopular. Here it is explained why. Evolutionary thinking, especially in the case of eukaryogenesis, is still dominated by anachronistic reasoning, in which highly derived protozoan organisms are seen as in some way representative of intermediate steps during eukaryotic evolution, hence poisoning the debate. This reasoning reflects a mind-set that ignores that Darwinian evolution is a fundamentally historic process. Numerous examples of this kind of erroneous reasoning are given, and some basic precautions against its use are formulated. Also see the video abstract here https://youtu.be/ekqtNleVJpU 相似文献