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1.
Adam C. Naj Honghuang Lin Badri N. Vardarajan Simon White Daniel Lancour Yiyi Ma Michael Schmidt Fangui Sun Mariusz Butkiewicz William S. Bush Brian W. Kunkle John Malamon Najaf Amin Seung Hoan Choi Kara L. Hamilton-Nelson Sven J. van der Lee Namrata Gupta Daniel C. Koboldt Anita L. DeStefano 《Genomics》2019,111(4):808-818
The Alzheimer's Disease Sequencing Project (ADSP) performed whole genome sequencing (WGS) of 584 subjects from 111 multiplex families at three sequencing centers. Genotype calling of single nucleotide variants (SNVs) and insertion-deletion variants (indels) was performed centrally using GATK-HaplotypeCaller and Atlas V2. The ADSP Quality Control (QC) Working Group applied QC protocols to project-level variant call format files (VCFs) from each pipeline, and developed and implemented a novel protocol, termed “consensus calling,” to combine genotype calls from both pipelines into a single high-quality set. QC was applied to autosomal bi-allelic SNVs and indels, and included pipeline-recommended QC filters, variant-level QC, and sample-level QC. Low-quality variants or genotypes were excluded, and sample outliers were noted. Quality was assessed by examining Mendelian inconsistencies (MIs) among 67 parent-offspring pairs, and MIs were used to establish additional genotype-specific filters for GATK calls. After QC, 578 subjects remained. Pipeline-specific QC excluded ~12.0% of GATK and 14.5% of Atlas SNVs. Between pipelines, ~91% of SNV genotypes across all QCed variants were concordant; 4.23% and 4.56% of genotypes were exclusive to Atlas or GATK, respectively; the remaining ~0.01% of discordant genotypes were excluded. For indels, variant-level QC excluded ~36.8% of GATK and 35.3% of Atlas indels. Between pipelines, ~55.6% of indel genotypes were concordant; while 10.3% and 28.3% were exclusive to Atlas or GATK, respectively; and ~0.29% of discordant genotypes were. The final WGS consensus dataset contains 27,896,774 SNVs and 3,133,926 indels and is publicly available. 相似文献
2.
Question: How can the U.S. National Vegetation Classification (USNVC) serve as an effective tool for classifying and mapping vegetation, and inform assessments and monitoring? Location: Voyageurs National Park, northern Minnesota, U.S.A and environs. The park contains 54 243 ha of terrestrial habitat in the sub-boreal region of North America. Methods: We classified and mapped the natural vegetation using the USNVC, with ‘alliance’and ‘association’as base units. We compiled 259 classification plots and 1251 accuracy assessment test plots. Both plot and type ordinations were used to analyse vegetation and environmental patterns. Color infrared aerial photography (1:15840 scale) was used for mapping. Polygons were manually drawn, then transferred into digital form. Classification and mapping products are stored in publicly available databases. Past fire and logging events were used to assess distribution of forest types. Results and Discussion: Ordination and cluster analyses confirmed 49 associations and 42 alliances, with three associations ranked as globally vulnerable to extirpation. Ordination provided a useful summary of vegetation and ecological gradients. Overall map accuracy was 82.4%. Pinus banksiana - Picea mariana forests were less frequent in areas unburned since the 1930s. Conclusion: The USNVC provides a consistent ecological tool for summarizing and mapping vegetation. The products provide a baseline for assessing forests and wetlands, including fire management. The standardized classification and map units provide local to continental perspectives on park resources through linkages to state, provincial, and national classifications in the U.S. and Canada, and to NatureServe's Ecological Systems classification. 相似文献
3.
Human monocytes synthesize large amounts of the second complement component (C2) after incubation with a T-lymphocyte product called monocyte complement stimulator (MCS). The human monocyte-like cell line, U937, also synthesizes C2 and can be stimulated to increase this synthesis by lymphokine-rich culture supernates. Additionally, phorbol myristate acetate (PMA), an agent which induces maturational changes in other macrophage-like cell lines, also stimulates C2 synthesis by U937 cells. Lymphokine and PMA stimulation of C2 secretion by U937 are both reversibly inhibitable by cycloheximide. At optimal concentrations for stimulation of C2 synthesis, PMA inhibits [3H]thymidine incorporation by U937 indicating that increased C2 is not due to increased numbers of U937 cells. 相似文献
4.
Question: Different plant growth forms may have distinctly different functioning in ecosystems. Association of phenological patterns with growth form will therefore help elucidate the role of phenology in an ecosystem. We ask whether growth forms of common vascular plants differ in terms of vegetative and flowering phenology, and if such phenological differences are consistent across environmental gradients caused by landscape‐scale topography. Location: A high‐latitude alpine landscape in Finnmark County, Norway (70°N). Methods: We assessed vegetative and flowering phenology repeatedly in five growth forms represented by 11 common vascular plant species across an altitudinal gradient and among differing slope aspects. Results: Species phenology clustered well according to growth form, and growth form strongly explained variation in both flowering and vegetative phenology. Altitude and aspect were poor predictors of phenological variation. Vegetative phenology of the growth forms, ranked from slowest to fastest, was in the order evergreen shrubs <sedges‐deciduous shrubs <grasses <forbs, while a reverse ranking was found for flowering phenology. Conclusion: Growth form‐specific phenological patterns are associated with fundamentally different abilities for resource acquisition and resource conservation. The weak effect of landscape‐scale topographic factors indicates that variation within growth forms is mainly influenced by local environmental factors not accounted for in this study. On the basis of these results, we argue that growth forms should be considered as predictors of phenology together with the customary use of topography and normalized difference vegetation index, especially when assessing the role of phenology in an ecosystem. 相似文献
5.
Limited spatial resources available for conservation lead to controversy about whether to apply single-species management
or ecosystemary approaches in order to maintain biodiversity. In this study I analyse changes in a community of breeding coastal
birds over a 90-year period, in order to examine whether natural processes on an unmanaged island are in accordance with requirements
to save endangered species. Both diversity and species richness of the community increased significantly over time, and evenness
increased after having been severely reduced by human impact. Diversity, evenness and species richness seemed to approach
an equilibrium in the past decade, but number of breeding pairs declined as a consequence of altered natural disturbance regime.
Species identity changed over time, with two initially very common species recently becoming locally extinct. These species
are of high conservation concern, and their disappearance causes a problem for the concept of naturalness on islands. I conclude
that natural processes need to be applied to the entire landscape in order to maintain dynamic processes that are essential
for the survival of these species. The natural changes on the island should not be interrupted, but rather demonstrated to
the public in order to increase conservation mindedness and gain support for changes in anthropogenic influences on a larger
spatial scale. 相似文献
6.
John H. Steele 《Ecological Research》1991,6(2):175-183
When marine and terrestrial ecological systems are compared at the same time scales, there are very great differences in their
relations with their physical environments. Similarities arise when comparisons are made at different time scales. There are
significant consequences for management. 相似文献
7.
《Fungal Ecology》2019
Mycorrhizas face unique anthropogenic stressors in urban environments, but the factors influencing their abundance in cities remains comparatively unstudied. Using green roofs as a model system, we built a conceptual model outlining the biotic and abiotic factors influencing arbuscular mycorrhizal (AM) fungal abundance in cities. We then tested this model and its multiple causal hypotheses with structural equation modeling using plant, soil, and AM fungal abundance measurements from 60 plots across 12 green roofs in Chicago, USA. The best direct predictor of AM fungal abundance was not plant cover, plant richness, or soil nutrients, but instead green roof age, suggesting temporal accumulation of AM fungal propagules in degraded urban environments. Soil P had the strongest total effect on AM fungal abundance both directly and via indirect promotion of plant cover. This study highlights knowledge gaps and challenges to studying the urban ecology of AM fungi. 相似文献
8.
Valuing mangrove ecosystem services: linking nutrient retention function of mangrove forests to enhanced agroecosystem production 总被引:1,自引:0,他引:1
Mangroves are highly productive wetland ecosystems strategically located at the interface between land and sea. They play
an important role in the biogeochemical cycles of the coastal environment, acting as sources of nutrients to adjacent marine
and terrestrial ecosystems through active and passive transport. We examined the nutrient contents in mangrove and nonmangrove
soils in and around the Bhitarkanika National Park, India and assessed whether the local agricultural producers were aware
of and placed a value on this contribution of mangrove forests in enhancing agroecosystem productivity. Soil samples from
both mangrove and nonmangrove areas were analysed and quantity of organic carbon, total nitrogen, available phosphorus and
potassium were derived. The replacement cost method was used to derive the value of nutrients in mangrove soils. We estimated
that each hectare of mangrove contains additional nutrients worth US$232.49 in comparison to nonmangrove areas. The difference
in nutrient content in mangrove versus nonmangrove areas gave the value of US$3.37 million for the nutrients in 145 km2 of mangrove forests. The agricultural producers were aware that mangrove forests act as a source of nutrients and were willing
to pay a higher price for the land adjoining mangrove forests. Around 92% of the producers ranked nutrient retention as a
secondary function of mangrove forests. Despite crop depredation from wild ungulates and conflict with salt water crocodiles
the agriculturist finds the benefit to cost ratio of mangrove forests high and more than 76% were in favour of mangrove restoration.
This study provides an opportunity to highlight the importance of mangrove ecosystems to the livelihoods of the local people
and the urgent need to sustain these through proper policy and market interventions. 相似文献
9.
10.
Thomas A. Kursar Catherina C. Caballero-George Todd L. Capson Luis Cubilla-Rios William H. Gerwick Maria V. Heller Alicia Ibañez Roger G. Linington Kerry L. McPhail Eduardo Ortega-Barría Luz I. Romero P. D. Coley 《Biodiversity and Conservation》2007,16(10):2789-2800
The limited international resources for economic aid and conservation can only mitigate poverty and losses of biodiversity.
Hence, developing nations must establish the capacity to resolve their problems. Additionally, policy-makers and donors need
to obtain scientific input on issues such as global change and ecosystem services. We propose that for nations rich in biodiversity,
ecosystem services derived from bioprospecting, or drug discovery, could contribute to economic development. In the case where
unstudied samples are shipped abroad for research, the chances of obtaining royalties are infinitesimally small. Therefore
developing nations will only realize benefits from bioprospecting through in-country research on their own biodiversity. Policy-makers
and donors have failed to appreciate the value of this approach. In order to provide an example of the inherent links between
conservation and sustainable economic development, we initiated a drug discovery effort in Panama that emphasizes local benefit.
As much of the drug discovery process as possible is conducted in Panamanian laboratories, providing jobs dependent on intact
biodiversity and enhancing local research and training. In short, research, plus the spin-offs from research, provide immediate
and long-lasting benefits to Panama. The connection between conservation and development has been highlighted in publicity
about the project in Panama’s urban media. This provides a constructive alternative to the perception the among the urban
populace that economic development inevitably competes with conservation. In summary, our program uses biodiversity to promote
human health as well as to support research capacity, economic development and conservation within Panama. The program provides
an example of the widely recognized but little developed concept of bioprospecting research as an ecosystem service. 相似文献