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1.
Jason M. Meyer Ailing Ji Lei Cai Deneys R. van der Westhuyzen 《Journal of lipid research》2014,55(8):1648-1656
Scavenger receptor-mediated uptake of oxidized LDL (oxLDL) is thought to be the major mechanism of foam cell generation in atherosclerotic lesions. Recent data has indicated that native LDL is also capable of contributing to foam cell formation via low-affinity receptor-independent LDL particle pinocytosis and selective cholesteryl ester (CE) uptake. In the current investigation, Cu2+-induced LDL oxidation was found to inhibit macrophage selective CE uptake. Impairment of selective CE uptake was significant with LDL oxidized for as little as 30 min and correlated with oxidative fragmentation of apoB. In contrast, LDL aggregation, LDL CE oxidation, and the enhancement of scavenger receptor-mediated LDL particle uptake required at least 3 h of oxidation. Selective CE uptake did not require expression of the LDL receptor (LDL-R) and was inhibited similarly by LDL oxidation in LDL-R−/− versus WT macrophages. Inhibition of selective uptake was also observed when cells were pretreated or cotreated with minimally oxidized LDL, indicating a direct inhibitory effect of this oxLDL on macrophages. Consistent with the effect on LDL CE uptake, minimal LDL oxidation almost completely prevented LDL-induced foam cell formation. These data demonstrate a novel inhibitory effect of mildly oxidized LDL that may reduce foam cell formation in atherosclerosis. 相似文献
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以籼型常规早稻中嘉早17为材料,于盆栽条件下采用人工气候箱控温,在水稻穗分化一次枝梗原基分化期(Ⅱ)与花粉母细胞减数分裂期(Ⅵ)进行17和20 ℃的低温胁迫处理,研究不同低温对水稻枝梗、颖花分化与退化及籽粒充实的影响.结果表明: 与对照相比,不同低温处理均显著降低每穗枝梗及颖花分化数和现存数,颖花现存数降幅为7.2%~12.4%,同时增加了枝梗和颖花的退化数,影响了花粉活性、花药开裂等花器官发育,导致籽粒充实不良,以17 ℃低温胁迫效应更明显.穗分化Ⅵ期低温处理总枝梗和颖花分化数与现存数低于穗分化Ⅱ期,但二次枝梗和颖花退化数较多,颖花退化数较穗分化Ⅱ期高11.6%;穗分化Ⅱ期低温处理穗部籽粒结实率显著低于穗分化Ⅵ期,降幅达3.7%,主要与花粉粒活性、柱头花粉散落数、花药开裂系数和籽粒充实度受低温影响较大有关.另外,穗分化Ⅱ、Ⅵ两时期受17 ℃低温胁迫效应大于20 ℃.综合穗分化两时期低温胁迫效应的差异,生产中需加强相应栽培措施的调控. 相似文献
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The occlusion of capillary vessels results in low oxygen tension in adjacent tissues which triggers a signaling cascade that culminates in neovascularization. Using bovine retinal capillary endothelial cells (BRCEC), we investigated the effects of short-term hypoxia on DNA synthesis, phosphotyrosine induction, changes in the expression of basic fibroblast growth factor receptor (bFGFR), protein kinase C (PKCα), heat shock protein 70 (HSP70), and SH2-containing protein (SHC). The effect of protein tyrosine kinase (PTK) and phosphatase inhibitors on hypoxia-induced phosphotyrosine was also studied. Capillary endothelial cells cultured in standard normoxic (pO2 = 20%) conditions were quiesced in low serum containing medium and then exposed to low oxygen tension or hypoxia (pO2 = 3%) in humidified, 5% CO2, 37°C, tissue culture chambers, on a time-course of up to 24 h. DNA synthesis was potentiated by hypoxia in a time-dependent manner. This response positively correlated with the cumulative induction of phosphotyrosine and the downregulation of bFGFR (Mr ~ 85 kDa). Protein tyrosine kinase inhibitors, herbimycin-A, and methyl 2,5-dihydroxycinnamate, unlike genistein, markedly blocked hypoxia-induced phosphotyrosine. Prolonged exposure of cells to phosphatase inhibitor, sodium orthovanadate, also blocked hypoxia-induced phosphotyrosine. The expression of HSP70, PKCα, and SHC were not markedly altered by hypoxia. Taken together, these data suggest that short-term hypoxia activates endothelial cell proliferation in part via tyrosine phosphorylation of cellular proteins and changes in the expression of the FGF receptor. Thus, endothelial cell mitogenesis and neovascularization associated with low oxygen tension may be controlled by abrogating signaling pathways mediated by protein tyrosine kinase and phosphatases. © 1995 Wiley-Liss, Inc. 相似文献
6.
Shuichi Nagashima Hiroaki Yagyu Ryuichi Tozawa Fumiko Tazoe Manabu Takahashi Tetsuya Kitamine Daisuke Yamamuro Kent Sakai Motohiro Sekiya Hiroaki Okazaki Jun-ichi Osuga Akira Honda Shun Ishibashi 《Journal of lipid research》2015,56(5):998-1005
Squalene synthase (SS) catalyzes the biosynthesis of squalene, the first specific intermediate in the cholesterol biosynthetic pathway. To test the feasibility of lowering plasma cholesterol by inhibiting hepatic SS, we generated mice in which SS is specifically knocked out in the liver (L-SSKO) using Cre-loxP technology. Hepatic SS activity of L-SSKO mice was reduced by >90%. In addition, cholesterol biosynthesis in the liver slices was almost eliminated. Although the hepatic squalene contents were markedly reduced in L-SSKO mice, the hepatic contents of cholesterol and its precursors distal to squalene were indistinguishable from those of control mice, indicating the presence of sufficient centripetal flow of cholesterol and/or its precursors from the extrahepatic tissues. L-SSKO mice showed a transient liver dysfunction with moderate hepatomegaly presumably secondary to increased farnesol production. In a fed state, the plasma total cholesterol and triglyceride were significantly reduced in L-SSKO mice, primarily owing to reduced hepatic VLDL secretion. In a fasted state, the hypolipidemic effect was lost. mRNA expression of liver X receptor α target genes was reduced, while that of sterol-regulatory element binding protein 2 target genes was increased. In conclusion, liver-specific ablation of SS inhibits hepatic cholesterol biosynthesis and induces hypolipidemia without increasing significant mortality. 相似文献
7.
气候变暖对东北三省春玉米严重低温冷害及种植布局的影响 总被引:31,自引:1,他引:30
以我国对气候变化较为敏感的东北三省为例,利用1961~2007年78个气象站的资料,结合玉米生长季温度距平冷害指标,分析了气候变暖对东北三省春玉米严重低温冷害及种植布局的影响.结果表明:(1)气候变暖背景下,从20世纪60年代到21世纪初,东北三省各地区春玉米严重冷害受害程度总体表现为减少趋势,但由于不同地方温度波动幅度较大,区域性的严重冷害发生频率也随之加大;就平均发生频率空间分布而言,1961~2007年严重冷害的平均发生频率表现为东北部高于西南部,最大值出现在黑龙江北部(38%),最低值在辽宁南部(4%).(2)气候变暖背景下,不同熟性玉米品种可种植界线明显北移东延,早熟品种逐渐被中、晚熟品种取代,中、晚熟品种可种植面积不断扩大.(3)随着不同熟性玉米品种种植区域北移东延,严重低温冷害出现频率明显增加,种植风险也在增大. 相似文献
8.
P. R. Warman 《Plant and Soil》1991,134(1):115-119
A field-size experiment was initiated in 1982 on an acid, low fertility Springhill silt loam to determine the effect of five
unfertilized green manure crops (alsike clover, sweet clover, single- and double-cut red clover, and buckwheat) on subsequent
oat production and soil fertility. The field was limed in 1982 and green manures were seeded (without fertilizer) in spring,
1983 in 1400 m2 strips randomly assigned within three treatment blocks. Plant tissue samples were taken from different locations in each
plot in the fall of 1983 and all crops were incorporated. In 1984 the field was separated into an upper and lower section
and each section received three rates of NPK fertilizer (0; 30-36-36; 60-72-72 kg ha-1) spread across the previous strips. Gary oats were seeded and at harvest were divided into grain and straw. The results indicated
significant effects of field sample location, green manure type and fertilizer level on oat yields. Buckwheat significantly
reduced oat production compared to the four clovers, while the highest fertilizer rate improved oat yields compared with the
other levels of fertilizers. Elemental analysis of the green manure crops and soil fertility was compared with data of the
same crops grown in more fertile, neutral soils. 相似文献
9.
Regulation of hepatic very low density lipoprotein (VLDL) assembly and maturation is crucial in controlling lipid homeostasis and in the development of metabolic disorders, including obesity, hepatic steatosis, and insulin resistance. Cideb, a member of cell death-inducing DFF45-like effector (CIDE) protein family, has been previously shown to promote VLDL lipidation and maturation. However, the precise subcellular location of Cideb-mediated VLDL lipidation and the factors modulating its activity remain elusive. In addition to its localization to endoplasmic reticulum (ER) and lipid droplets (LD), we observed that Cideb was also localized to the Golgi apparatus. Mature and lipid-rich VLDL particles did not accumulate in the Golgi apparatus in Cideb(-/-) livers. Interestingly, we observed that hepatic perilipin 2/adipose differentiation-related protein (ADRP) levels were markedly increased in Cideb(-/-) mice. Liver-specific knockdown of perilipin 2 in Cideb(-/-) mice resulted in the reduced accumulation of hepatic triglycerides (TAG), increased VLDL-TAG secretion, and the accumulation of mature TAG-rich VLDL in the Golgi apparatus. These data reveal that Cideb and perilipin 2 play opposing roles in controlling VLDL lipidation and hepatic lipid homeostasis. 相似文献
10.
化学催熟剂对油菜角果叶绿素含量及抗氧化酶系统的影响 总被引:2,自引:0,他引:2
采用大田试验研究了两种化学催熟剂(敌草快和农达)对生长后期油菜角果的叶绿素含量、抗氧化酶系统(CAT、SOD、POD活性)、细胞膜透性及MDA含量的影响.结果表明:采用敌草快催熟,油菜角果皮叶绿素含量下降,SOD、POD、CAT活性及细胞膜透性和MDA含量显著提高,导致角果膜脂过氧化,且作用强度随处理浓度的增加而增加;采用农达催熟,油菜角果皮叶绿素含量所受影响较小,SOD、POD和CAT活性上升缓慢,细胞膜透性和MDA含量增加不明显.随着催熟时间的推移,油菜角果保护酶活性受到不同程度的抑制,这可能与催熟剂干扰酶系统分子结构有关. 相似文献