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The reconstruction of musculoskeletal defects is a constant challenge for orthopaedic surgeons. Musculoskeletal injuries such as fractures, chondral lesions, infections and tumor debulking can often lead to large tissue voids requiring reconstruction with tissue grafts. Autografts are currently the gold standard in orthopaedic tissue reconstruction; however, there is a limit to the amount of tissue that can be harvested before compromising the donor site. Tissue engineering strategies using allogeneic or xenogeneic decellularized bone, cartilage, skeletal muscle, tendon and ligament have emerged as promising potential alternative treatment. The extracellular matrix provides a natural scaffold for cell attachment, proliferation and differentiation. Decellularization of in vitro cell-derived matrices can also enable the generation of autologous constructs from tissue specific cells or progenitor cells. Although decellularized bone tissue is widely used clinically in orthopaedic applications, the exciting potential of decellularized cartilage, skeletal muscle, tendon and ligament cell-derived matrices has only recently begun to be explored for ultimate translation to the orthopaedic clinic.  相似文献
2.
侯楠  朱力 《生物磁学》2011,(2):381-383
去细胞基质在组织工程及再生医学的大量应用为解决组织器官的修复和重建等难题带来了希望。去细胞方法大致可以分为三类:化学处理法、物理处理法及酶学处理法,且已经应用于组织工程及再生医学的各个方面。本文总结并分类目前常用的去细胞方法及其在组织工程各方面的应用,对目前国内外常用的去细胞方法及其在组织工程及再生医学中的应用进行回顾总结与分析。  相似文献
3.
去细胞基质在组织工程及再生医学的大量应用为解决组织器官的修复和重建等难题带来了希望。去细胞方法大致可以分为三类:化学处理法、物理处理法及酶学处理法,且已经应用于组织工程及再生医学的各个方面。本文总结并分类目前常用的去细胞方法及其在组织工程各方面的应用,对目前国内外常用的去细胞方法及其在组织工程及再生医学中的应用进行回顾总结与分析。  相似文献
4.
Toad bone not only contains the rich cartilage‐like matrix but also presents low immunogenicity. It is inferred that decellularized toad bone matrix (dBECM) may provide the more profitable osteoinductive microenvironment for mesenchymal stem cells (MSCs) to promote the repair of bone defects. Herein, a hollow bone‐inspired tube is first made from hydroxyapatite (HA) and poly (γ‐glutamic acid) (PGA), and then MSCs/dBECM hydrogel is uniformly filled to its central cavity, constructing a biomimetic bone (dBECM + MSCs − PGA + HA). In vitro scratch and transwell experiments show that dBECM hydrogel not only effectively promotes migration and proliferation of MSCs but also induces their osteogenic differentiation. Moreover, the less inflammatory macrophages infiltrate at rat skin after subcutaneously injecting dBECM hydrogel, indicating its low potential for inflammatory attack. After implanting dBECM + MSCs − PGA + HA to critical radius defect of rabbit, X‐ray and CT imaging shows that the cortex is effectively regenerated and the medullary cavity recanalization is completed at 20 weeks. Moreover, the expression of Collagen‐II and OCN are obviously increased in the defect after implanting dBECM + MSCs − PGA + HA. The therapeutic mechanism of dBECM + MSCs − PGA + HA scaffold are highly associated with the enhanced angiogenesis. Collectively, the biomimetic dBECM + MSCs − PGA + HA scaffold may be a promising strategy to improve radius defect healing efficiency.  相似文献
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Background aims

Retinal progenitor cells (RPCs) are a promising cell therapy treatment for retinal degenerative diseases. However, problems with limited proliferation ability and differentiation preference toward glia rather than neurons restrict the clinical application of these RPCs. The extracellular matrix (ECM) has been recognized to provide an appropriate microenvironment to support stem cell adhesion and direct cell behaviors, such as self-renewal and differentiation.

Methods

In this study, decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was manufactured using a chemical agent method (0.5% ammonium hydroxide Triton + 20?mmol/L NH4OH) in combination with a biological agent method (DNase solution), and the resulting DMA were evaluated by scanning electron microscopy (SEM) and immunocytochemistry. The effect of DMA on RPC proliferation and differentiation was evaluated by quantitative polymerase chain reaction, Western blot and immunocytochemistry analysis.

Results

DMA was successfully fabricated, as demonstrated by SEM and immunocytochemistry. Compared with tissue culture plates, DMA may effectively enhance the proliferation of RPCs by activating Akt and Erk phosphorylation; when the two pathways were blocked, the promoting effect was reversed. Moreover, DMA promoted the differentiation of RPCs toward retinal neurons, especially rhodopsin- and recoverin-positive photoreceptors, which is the most interesting class of cells for retinal degeneration treatment.

Conclusions

These results indicate that DMA has important roles in governing RPC proliferation and differentiation and may contribute to the application of RPCs in treating retinal degenerative diseases.  相似文献
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