Residues and contaminants in medicinal herbs—A review

With the increasing popularity and use of medicinal herbs, their global demand has gained momentum. Developing countries, including China, India and South East Asian (SEA) countries, are the centres of origin and major global suppliers for most of these traditionally used medicinal herbs. One of the factors affecting the quality of these herbs is the contamination of heavy metals, mycotoxins, pesticide residues, polycyclic aromatic hydrocarbons (PAHs) and fumigants. These contaminants can accumulate during the cultivation, storage and processing of herbs and may have adverse effects on consumer health. There have been various reports regarding the presence of these contaminants in medicinal herbs. This review discusses the important contaminants of medicinal herbs, the frequency and magnitude of their occurrences, the potential causes of contamination and their regulatory limits in medicinal herbs. The major challenge in the international trade of medicinal herbs is the lack of common guidelines, regulatory measures and monitoring body to strictly enforce their regulation.     

Mercury contamination and stable isotopes reveal variability in foraging ecology of generalist California gulls

Environmental contaminants are a concern for animal health, but contaminant exposure can also be used as a tracer of foraging ecology. In particular, mercury (Hg) concentrations are highly variable among aquatic and terrestrial food webs as a result of habitat- and site-specific biogeochemical processes that produce the bioaccumulative form, methylmercury (MeHg). We used stable isotopes and total Hg (THg) concentrations of a generalist consumer, the California gull (Larus californicus), to examine foraging ecology and illustrate the utility of using Hg contamination as an ecological tracer under certain conditions. We identified four main foraging clusters of gulls during pre-breeding and breeding, using a traditional approach based on light stable isotopes. The foraging cluster with the highest δ¹⁵N and δ³⁴S values in gulls (cluster 4) had mean blood THg concentrations 614% (pre-breeding) and 250% (breeding) higher than gulls with the lowest isotope values (cluster 1). Using a traditional approach of stable-isotope mixing models, we showed that breeding birds with a higher proportion of garbage in their diet (cluster 2: 63–82% garbage) corresponded to lower THg concentrations and lower δ¹⁵N and δ³⁴S values. In contrast, gull clusters with higher THg concentrations, which were more enriched in ¹⁵N and ³⁴S isotopes, consumed a higher proportion of more natural, estuarine prey. δ³⁴S values, which change markedly across the terrestrial to marine habitat gradient, were positively correlated with blood THg concentrations in gulls. The linkage we observed between stable isotopes and THg concentrations suggests that Hg contamination can be used as an additional tool for understanding animal foraging across coastal habitat gradients.     

Heavy metal pollution in surface water and sediment: A preliminary assessment of an urban river in a developing country

The concentration and chemical fractionation of globally alarming six heavy metals (Cr, Ni, Cu, As, Cd and Pb) were measured in surface water and sediment of an urban river in Bangladesh. The decreasing trend of metals were observed in water as Cr > Cu > As > Ni > Pb > Cd and in sediment as Cr > Ni > Cu > Pb > As > Cd. The level of studied metals exceeded the safe limits of drinking water, indicated that water from this river is not safe for drinking and/or cooking purposes. However, the investigated metals showed low mobility except for Cd and Pb which could pose a severe threat to the aquatic environment. Contamination factor (CF) and geoaccumulation index (I_geo) demonstrated that most of the sediment samples were moderately to heavily contaminated by Cr, As, Cd and Pb. The pollution load index (PLI) values were above one (>1) indicates progressive deterioration of the sediment quality. The extent of pollution by heavy metals in the river Korotoa implies that the condition is much frightening to the biota and inhabitants in the vicinity of the river as well.     

Biochemical composition of the parasitic amphipod Hyperia galba in relation to age and starvation

Summary Hyperia galba was collected in the waters around Helgoland and in the Weser-Elbe-estuary during autumn. Its mode of life is a temporary but obligatory parasitism. The basal biochemical composition of the adults was analyzed in detail and related to the fresh, dry, and ash-free dry weight as well as sex and body length. Hyperia galba (males/females) consists of 85/86% water; the dry matter contains approximately 64/61% protein, 10/11% lipid, 1.2/1.5% carbohydrate, 10/8% chitin, and 23/23% ash. The analyses of basal elemental composition yielded approximately 38% C, 9% N and 6% H. Under natural conditions, individuals may encounter at least two periods of food shortage during their lives. Therefore, the analyses were carried out on individuals of different developmental stages also under food deprivation. The data are discussed with respect to the special mode of life of the species and compared with those found by other authors in several related crustacean species.Abbreviations AFDW ash-free dry weight - BL body length - DW dry weight - SD standard deviation - WW wet weight     

Enhancing native chemical ligation for challenging chemical protein syntheses

Native chemical ligation has enabled the chemical synthesis of proteins for a wide variety of applications (e.g., mirror-image proteins). However, inefficiencies of this chemoselective ligation in the context of large or otherwise challenging protein targets can limit the practical scope of chemical protein synthesis. In this review, we focus on recent developments aimed at enhancing and expanding native chemical ligation for challenging protein syntheses. Chemical auxiliaries, use of selenium chemistry, and templating all enable ligations at otherwise suboptimal junctions. The continuing development of these tools is making the chemical synthesis of large proteins increasingly accessible.     

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

The cantilever sensor, which acts as a transducer of reactions between model bacterial cell wall matrix immobilized on its surface and antibiotic drugs in solution, has shown considerable potential in biochemical sensing applications with unprecedented sensitivity and specificity^1-5. The drug-target interactions generate surface stress, causing the cantilever to bend, and the signal can be analyzed optically when it is illuminated by a laser. The change in surface stress measured with nano-scale precision allows disruptions of the biomechanics of model bacterial cell wall targets to be tracked in real time. Despite offering considerable advantages, multiple cantilever sensor arrays have never been applied in quantifying drug-target binding interactions.Here, we report on the use of silicon multiple cantilever arrays coated with alkanethiol self-assembled monolayers mimicking bacterial cell wall matrix to quantitatively study antibiotic binding interactions. To understand the impact of vancomycin on the mechanics of bacterial cell wall structures^1,6,7. We developed a new model¹ which proposes that cantilever bending can be described by two independent factors; i) namely a chemical factor, which is given by a classical Langmuir adsorption isotherm, from which we calculate the thermodynamic equilibrium dissociation constant (K_d) and ii) a geometrical factor, essentially a measure of how bacterial peptide receptors are distributed on the cantilever surface. The surface distribution of peptide receptors (p) is used to investigate the dependence of geometry and ligand loading. It is shown that a threshold value of p ~10% is critical to sensing applications. Below which there is no detectable bending signal while above this value, the bending signal increases almost linearly, revealing that stress is a product of a local chemical binding factor and a geometrical factor combined by the mechanical connectivity of reacted regions and provides a new paradigm for design of powerful agents to combat superbug infections.     

Association of loganin contents with the genetic characterization of natural populations of Palicourea rigida Kunth determined by AFLP molecular markers

Extracts of the medicinal plant Palicourea rigida Kunth, popularly known as douradinha, are widely used for treating urinary tract disorders. Unfortunately, nowadays this is one of the species endemic to Brazilian Cerrado that is at greatest risk of extinction.The aim of the this work was to use AFLP molecular markers to determine the genetic structure and diversity of eight natural populations of P. rigida and to associate their genetic characteristics with loganin production in order to obtain provide relevant information to promote programs for the conservation of this valuable medicinal plant.A total of 120 polymorphic bands were scored and higher proportion of genetic diversity was found in inter-populations (64%) rather than in intra-populations (36%). F_st value was found to be significantly greater than zero (0.3601), demonstrating the complex genetic structure of P. rigida populations. Accessions collected from Cristalina, GO, showed higher percentage of polymorphic loci (65.5%) and the highest genetic diversity. Analysis of Molecular variance (AMOVA) demonstrated 63.9% of intra-population genetic variation. The lowest genetic variability was detected among accessions from the population found in Sacramento, MG. No spatial standard was observed for P. rigida population, suggesting a partially isolated island model. It was observed a minor but significant positive correlation (r = 0.22) between chemical and genetic matrices. The association between chemical and genetic data indicated that environmental factors promoted the loganin production in populations growing in Luziânia, GO, and therefore accessions from those populations should be considered as prime material for initiating the conservation process of P. rigida.     

Orientation and Shape Discrimination in Juveniles and Adults of the Mangrove Crab Aratus pisonii (H. Milne Edwards, 1837): Effect of Predator and Chemical Cues

Behavioral responses of juveniles and adults of the mangrove crab Aratus pisonii (H. Milne Edwards, 1837) to black geometric shapes of equal surface area was measured. Crabs were tested either in presence or absence of chemicals generated from two common predator species, the portunid crab Callinectes ornatus Ordway, and the soapfish Haemulon aurolineatum Cuvier, 1830. The present study tested the hypothesis that A. pisonii (1) has the capacity to orient to visual cues; (2) it discriminates between different visual objects based on a combination of chemical and visual information and (3) this behavior changes with age. When presented with single black targets in background water, juveniles oriented toward all shapes. This behavioral response was interpreted as visual orientation toward potential shelter. Among shapes, juveniles showed preference for the vertical rectangle, probably due to the recognition of natural visual elements like mangrove roots. In predator conditioned water, juveniles exhibited a stronger response than in background water. Thus, juveniles were able to detect by odor the potential presence of predators. Change in responsiveness between adults and juveniles was also demonstrated.     

Factors influencing production of cationic starches

The main factors – the amount of catalyst NaOH, the temperature and composition of reaction mixture – influencing the effectiveness of starch cationization with glycidyltrimethylammonium chloride (GTAC) were investigated. It was found that cationic or cross-linked cationic starches with preserved microgranules, a degree of substitution from 0.2 to 0.85 and reaction efficiency from 82% to 93% could be obtained during etherification of starch or cross-linked starch with a mixture containing GTAC, “free” water and 0.04–0.08 mol/AGU sodium hydroxide in heterogeneous condition. The activation energy of the GTAC main reaction is lower than that of the side reactions, thus starch cationization at a lower temperature proceeds with higher reaction efficiency.The amount of NaOH in the cationization mixture has a great influence on the rate of the main and side reactions of GTAC. Only the main reaction – cationization of starch – proceeds when all the NaOH present in the reaction mixture is adsorbed from the liquid phase by the starch. The duration of this “first reaction stage” decreases with increasing reaction temperature and the amount of NaOH in the reaction mixture. NaOH present in the liquid phase of the reaction mixture catalyzes the side reactions of GTAC and changes their character.     

Separation and identification of chemical constituents of Morchella conica isolated from Guizhou Province China

Morchella conica, isolated from Southwest China, was identified based on its microstructure and ITS rDNA sequence. Nine chemical constituents (1–9) were separated from M. conica through fermentation, and their structures were identified according to spectroscopic data and chemical evidence as follows: two unsaturated fatty acid and ester (1–2), three sterols (3–5), one aromatic carboxylic acid (6) and derivatives (7), one base (8), and chlorinated aromatic esters (9). Subsequently, the chemotaxonomic significance of Compounds 2, 7, and 9, which are the first to be reported in Morchella spp., was summarized.