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1.
《Systematic and applied microbiology》2020,43(2):126064
The ability to grow by anaerobic CO oxidation with production of H2 from water is known for some thermophilic bacteria, most of which belong to Firmicutes, as well as for a few hyperthermophilic Euryarchaeota isolated from deep-sea hydrothermal habitats. A hyperthermophilic, neutrophilic, anaerobic filamentous archaeon strain 1505 = VKM B-3180 = KCTC 15798 was isolated from a terrestrial hot spring in Kamchatka (Russia) in the presence of 30% CO in the gas phase. Strain 1505 could grow lithotrophically using carbon monoxide as the energy source with the production of hydrogen according to the equation CO + H2O → CO2 + H2; mixotrophically on CO plus glucose; and organotrophically on peptone, yeast extract, glucose, sucrose, or Avicel. The genome of strain 1505 was sequenced and assembled into a single chromosome. Based on 16S rRNA gene sequence analysis and in silico genome-genome hybridization, this organism was shown to be closely related to the Thermofilum adornatum species. In the genome of Thermofilum sp. strain 1505, a gene cluster (TCARB_0867-TCARB_0879) was found that included genes of anaerobic (Ni,Fe-containing) carbon monoxide dehydrogenase and genes of energy-converting hydrogenase ([Ni,Fe]-CODH–ECH gene cluster). Compared to the [Ni,Fe]-CODH–ECH gene clusters occurring in the sequenced genomes of other H2-producing carboxydotrophs, the [Ni,Fe]-CODH–ECH gene cluster of Thermofilum sp. strain 1505 presented a novel type of gene organization. The results of the study provided the first evidence of anaerobic CO oxidation coupled with H2 production performed by a crenarchaeon, as well as the first documented case of lithotrophic growth of a Thermofilaceae representative. 相似文献
2.
Land use changes sharply under rapid urbanization, yet its ecological and environmental effects are often neglected in land use decisions. Using the case of Hangzhou, China, we analyze the ecological and environmental effects of land use changes, including ecosystem services value (ESV) and carbon emissions, based on Landsat TM images from 1995, 2000, 2005, 2010, and 2014. We found significant ecological and environmental effects of land use changes under rapid urbanization. The value of ecosystem services in Hangzhou decreased from 546.7 million USD in 1995 to 448.97 million USD in 2014, and the ratio of ESV to GDP decreased from 5.8% to 0.6%. The net carbon emissions associated with land use changes increased from 4.26 million tons in 1995 to 15.10 million tons in 2014, mainly due to the increase of built-up land carbon emissions and the decrease of forest land carbon sink. The ESV is unevenly distributed spatially and low ESV spread from the central to the peripheral area. We use scenario analysis to illustrate that economic growth and environmental protection could be coordinated by bringing ecological and environmental effects into land use decisions. 相似文献
3.
Preparations of rat liver sinusoidal plasma membrane have been tested for their ability to metabolize the hepatotoxin carbon tetrachloride (CCl4) to reactive free radicals in vitro and compared in this respect with standard preparations of rat liver microsomes. The sinusoidal plasma membranes were relatively free of endoplasmic reticulum-associated activities such as the enzymes of the cytochrome P450 system and glucose-6-phosphatase. CCl4 metabolism was measured as (i) covalent binding of [14C]-CCl4 to membrane protein, (ii) electron spin resonance spin-trapping of CCl3. radicals and (iii) CCl4-induced lipid peroxidation. By all of these tests, purified sinusoidal plasma membranes were found unable to metabolize CCl4. The fatty acid composition of the plasma membranes was almost identical to that of the microsomal preparation and both membrane fractions exhibited similar rates of the lipid peroxidation that was stimulated non-enzymically by gamma-radiation or incubation with ascorbate and iron. The absence of CCl4-induced lipid peroxidation in the plasma membranes seems to be due, therefore, to an absence of CCl4 activation rather than an inherent resistance to lipid peroxidation. We conclude that damage to the hepatocyte plasma membrane during CCl4 intoxication is not due to a significant local activation of CCl4 to CCl3. within that membrane. 相似文献
4.
L.S. Panchakarla Author VitaeAuthor Vitae C.N.R. Rao Author Vitae 《Inorganica chimica acta》2010,363(15):4163-4174
Multi-walled, single-walled and double-walled carbon nanotubes as well as graphene can be doped with boron and nitrogen. B2H6 has been generally used as the boron source while NH3 or pyridine is employed as the nitrogen source. Doping carbon nanotubes and graphene with boron and nitrogen brings about significant changes in the electronic structure and properties. Such doping not only results in desirable properties but also allows manipulation of properties for specific purposes. Doping with boron- and nitrogen-causes marked changes in the Raman spectra of the carbon nanostructures. In this article, we present the synthesis, characterization and properties of boron- and nitrogen-doped carbon nanotubes and graphene. 相似文献
5.
There was little release of extractable SO4-S during four weeks from CS2 applied by injecting into two S-deficient soils. In this incubation experiment, the rate of CS2 was 30 μg S g−, placement was injection at 9 cm depth, soil temperature was 20°C, and soil moisture tension was 33 kPa. The yield of barley
forage after seven weeks in the greenhouse showed only small increases from 10 or 30 μg S g−1 of CS2 as compared to Na2SO4, on the two soils.
While CS2 supplied little plant available S in the short term, it was an effective inhibitor of nitrification. In the laboratory, or
in the field, the injection of CS2 (with N fertilizers) at a point 9 cm into the soils either stopped or reduced nitrification. In one laboratory experiment,
35 μg of CS2 g−1 of soil with urea reduced nitrification for at least four weeks; and in another experiment 20 μg of CS2 g−1 of soil with aqua NH3 nearly or completely inhibited nitrification at 20 days. In two field experiments, 3 and 12 μg of CS2 g−1 of soil (or 6 and 24 kg ha−1) with aqua NH3 inhibited nitrification from October to the subsequent May. In addition, CS2 reduced the amount of ammonium produced from the soil N, both in these two field experiments and in the laboratory experiments.
That is to say, CS2 injected at a point, inhibited both nitrification and ammonification.
In other field experiments, CS2 at a rate of 10 kg ha−1 was injected in bands 9 cm deep with urea in October, and by May there was still reduced nitrification. Less than half of
the fall-applied urea alone was recovered as mineral N, but with the application of CS2 the recovery was increased to three-quarters.
The yield and N uptake of barley grain was increased where fall-applied banded urea or aqua NH3 received banded CS2, (NH4)2CS3, or K2CS3. The average increase in yield from fall-applied fertilizer, from inhibitor with fall-applied fertilizer, and from spring-applied
fertilizer was 800, 1370, and 1900 kg ha−1, respectively. In the same order, the apparent % recovery of fertilizer N in grain was 24, 42, and 60. 相似文献
6.
Structural equation modelling reveals plant-community drivers of carbon storage in boreal forest ecosystems 总被引:1,自引:0,他引:1
Boreal forest ecosystems are important drivers of the global carbon (C) cycle by acting as both sinks and sources of atmospheric CO2. While several factors have been proposed as determining the ability of boreal forest to function as C sinks, little is known about their relative importance. In this study, we applied structural equation modelling to a previously published dataset involving 30 boreal-forested islands that vary greatly in their historic fire regime, in order to explore the simultaneous influence of several factors believed to be important in influencing above-ground, below-ground and total ecosystem C accumulation. We found that wildfire was a major driver of ecosystem C sequestration, and exerted direct effects on below-ground C storage (presumably through humus combustion) and indirect effects on both above-ground and below-ground C storage through altering plant-community composition. By contrast, plant diversity influenced only below-ground C storage (and even then only weakly), while net primary productivity and decomposition had no detectable effect. Our results suggest that while boreal forests have great potential for storing significant amounts of C, traits of dominant plant species that promote below-ground C accumulation and the absence of wildfire are the most important drivers of C sequestration in these ecosystems. 相似文献
7.
J. L. M. Huntjens 《Plant and Soil》1979,53(4):529-534
Summary A differential infrared CO2 analyser combined with a 12 channel gas handling system have been used for the measurement of CO2 evolution rates of soil samples. A constant flow of air over the soil was maintained during the incubation period. Automatic sequential measurement and recording of the increase of the CO2 content of the flushed air of the 12 channels lasted 24 min with a dwell time of 2 min per channel. This technique has proven to be very useful for accurate and rapid measurement of the biological activities in untreated and treated soil. 相似文献
8.
ABSTRACT. A non-dispersive infrared gas analyser equipped with a Luft-type sonic detector and flow-through reference cell was automated to monitor the total volume of carbon dioxide (CO2) respired by single insects or groups of insects. The infrared analyser was interfaced with an integrator for quantification, a microprocessor to control intermittent air flow through the insect respiration chambers, and a microcomputer for data storage and reduction. This technique has been used to monitor the CO2 Output of diapausing and non-diapausing mature fifth instar larvae and of developing pupae of the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). The resulting data were accurate, quantitative and reproducible. 相似文献
9.
10.
Effects of ozone and elevated atmospheric carbon dioxide on carbohydrate metabolism of spruce needles. Catabolic and detoxification pathways 总被引:5,自引:0,他引:5
We have studied the effects of ozone, carbon dioxide and ozone combined with carbon dioxide fumigations on catabolic and detoxification pathways in spruce ( Picea abies [L.] Karst.) needles. The results obtained showed an increase in the activities of three enzymes involved in the detoxification pathway, superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (AscPOD, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) when trees were exposed to ozone and to ozone‐carbon dioxide treatments. In these two treatments, the fraction of SOD activity due to the chloroplastic isoform was increased (1.5‐fold). In the needles of trees exposed to ozone and to ozone‐carbon dioxide fumigation, an increase in the activities of glucose‐6‐phosphate dehydrogenase (G‐6‐PDH, EC 1.1.1.49) showed that the cell had the capacity to produce more NADPH necessary for the detoxification. Stimulation of other enzymes of catabolic pathways (fumarase [EC 4.2.1.2], phosphofructokinase [PFK, EC 2.7.1.1] and phosphoenolpyruvate carboxylase [PEPC, EC 4.1.1.31]), was also observed making it possible for the cell to provide the reducing power necessary for detoxification as well as energy and carbon skeletons involved in the repair processes.
When carbon dioxide alone was applied, no effects could be detected on these enzyme activities. However, when carbon dioxide was combined with ozone, the effect of ozone on trees was less than that induced by ozone alone, suggesting that elevated atmospheric carbon dioxide concentrations may to some extent protect plants from ozone injury. 相似文献
When carbon dioxide alone was applied, no effects could be detected on these enzyme activities. However, when carbon dioxide was combined with ozone, the effect of ozone on trees was less than that induced by ozone alone, suggesting that elevated atmospheric carbon dioxide concentrations may to some extent protect plants from ozone injury. 相似文献