全文获取类型
收费全文 | 947篇 |
免费 | 38篇 |
国内免费 | 86篇 |
专业分类
1071篇 |
出版年
2024年 | 3篇 |
2023年 | 10篇 |
2022年 | 14篇 |
2021年 | 8篇 |
2020年 | 15篇 |
2019年 | 23篇 |
2018年 | 17篇 |
2017年 | 21篇 |
2016年 | 29篇 |
2015年 | 32篇 |
2014年 | 34篇 |
2013年 | 38篇 |
2012年 | 23篇 |
2011年 | 37篇 |
2010年 | 36篇 |
2009年 | 45篇 |
2008年 | 71篇 |
2007年 | 58篇 |
2006年 | 44篇 |
2005年 | 58篇 |
2004年 | 49篇 |
2003年 | 41篇 |
2002年 | 23篇 |
2001年 | 22篇 |
2000年 | 25篇 |
1999年 | 16篇 |
1998年 | 27篇 |
1997年 | 19篇 |
1996年 | 25篇 |
1995年 | 23篇 |
1994年 | 22篇 |
1993年 | 17篇 |
1992年 | 14篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1989年 | 13篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 16篇 |
1985年 | 18篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1978年 | 8篇 |
1977年 | 3篇 |
1968年 | 1篇 |
1965年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有1071条查询结果,搜索用时 15 毫秒
1.
Levels of genetic diversity at different stages of the domestication cycle of interior spruce in British Columbia 总被引:3,自引:0,他引:3
M. U. Stoehr Y. A. El-Kassaby 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):83-90
Concerns over the reductionist nature of the domestication of forest-tree species focus on the possibility of potential genetic
erosion during this process. To address these concerns, genetic diversity assessments in a breeding zone the Province of British
Columbia “interior” spruce (Picea glauca×engelmanni) program was conducted using allozyme markers. Genetic-variation comparisons were made between natural and production (seed
orchard) populations as well as seed and seedling crops produced from the same breeding zone’s seed orchard. The natural population
sample consisted of a total of 360 trees representing three stands within each of three watersheds present in the Shuswap-Adams
low-elevation zone of interior British Columbia. Small amounts of genetic differentiation were observed among the nine natural
populations (4%) and this was attributable to extensive gene flow Consequently, the sum of these nine populations was considered as a baseline for the genetic variation present in the breeding
zone. The comparisons between the seed orchard and the breeding zone produced a similar percentage of polymorphic loci while the expected hetrozygosity (0.207 vs 0.210) and the average number of alleles per locus (2.7 vs 2.4) were slightly lower in the seed orchard. A total
of seven natural populations’ rare alleles were not present in the orchard population, while one allele was unique to the orchard. The %P increased to 70.6% in the seedlot, but dropped to the natural populations level (64.7%) in the plantation. The observed increase
in %P was a result of pollen contamination in the orchard. It is suspected that the reduction in the plantation was caused by an
unintentional selection in the nursery. Simulated roguing in the orchard did not drastically reduce even if up to 50% of the orchard’s clones were rogued. However, roguing was associated with a reduction in the average number
of alleles per locus (i.e., sampling effect).
Received: 2 January 1996 / Accepted: 24 May 1996 相似文献
2.
The occurrence and frequency of 2n pollen in 2x, 4x,and 6x wild,tuber-bearing Solanum species from Mexico,and Central and South America 总被引:1,自引:0,他引:1
K. Watanabe S. J. Peloquin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,82(5):621-626
Summary The occurrence of 2n pollen-producing plants was investigated in 187 plant introductions (PIs) of 38 wild species of tuber-bearing Solanum. These 2x, 4x, and 6x species are from Mexico, and Central and South America. The determination of 2n pollen-producing plants was conducted using acetocarmine glycerol. Plants with more than 1% large-size pollen were regarded as 2n pollen-producing plants. 2n pollen-producing plants were identified in the following species: 10 out of 12 Mexican 2x species, seven of nine South American 2x species, seven of seven Mexican and Central American 4x species, five of five South American 4x species, and five of five Mexican 6x species. The frequency of 2n pollen-producing plants varied among species at the same ploidy level, but the range of frequency, generally between 2 and 10% among species, was similar over different ploidy levels. The general occurrence of 2n pollen in both 2x and polyploid species, which are evolutionarily related, is evidence that the mode of polyploidization in tuber-bearing Solanums is sexual polyploidization. Furthermore, the frequencies of 2n pollen-producing plants in autogamous disomic polyploid species were not markably different from those of their related diploid species. It is thought that the frequent occurrence of 2n gametes with autogamy tends to disturb the fertility and consequently reduce fitness of polyploids. Thus, we propose that the breeding behavior of polyploids and the occurrence of 2n gametes may be genetically balanced in order to conserve high fitness in polyploid species in tuberbearing Solanum.Paper No. 3114 from the Laboratory of Genetics. Research supported by the College of Agriculture and Life Sciences; International Potato Center; USDA, SEA, CGRO 84-CRCR-1-1389; and Frito Lay, Inc. 相似文献
3.
G. Pellegrino D. Caimi M. E. Noce A. Musacchio 《Plant Systematics and Evolution》2005,251(2-4):119-129
The rewardless orchid Dactylorhiza sambucina shows a stable flower colour polymorphism, with both yellow- and red-flowered morphs growing sympatrically. Pollination biology and breeding system were investigated to examine the effects of density of plants, colour polymorphism, inflorescence dimension, and flower position within inflorescence on male and female reproductive success in three natural populations of D. sambucina. There were significant differences among sites in the number of pollinia removed and in fruit set per inflorescence. Number of removed pollinia and capsule production in D. sambucina were independent from flower and inflorescence size or flower position. As a whole, the red morphs showed the highest number of capsules produced, while the yellow morphs had the greatest male success. The relative male and female reproductive success were independent from plant density but were significantly correlated with the yellow morph frequency at the population level. Overall, our findings show that the contribution to the total reproductive success deriving from the two colour morphs does not conform with the predictions of negative frequency-dependent selection. 相似文献
4.
Sonja T. Chou 《中国实验动物学杂志》2011,(1):5-11
2009年11月份在西安举行由中国实验动物学会发起的实验动物医药技术培训研讨会上,本人做了关于实验小鼠种群管理的演讲。其主题涵盖了小鼠繁殖性能的维护和基因工程种群的管理。本篇文章的目的在于强调其所涵盖的材料并为进一步研究提供参考。如若对本文所讨论的内容希望有更深刻的理解,这篇文章所引用的参考资料值得进一步的阅读。另外本文未涉及远交系小鼠的繁殖培育和基因管理,但如有需要可以联系本文作者,可推荐需阅读的材料清单。 相似文献
5.
A. Gallais A. J. Wright 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1980,57(2):81-87
Summary Genetic effects for varietal value are defined at the level of the population of k-parent synthetic varieties. A simple expression for the total variance among synthetics arises directly from these definitions. A general expression for the covariance among related synthetics is given. Genetic effects are also defined in a completely general way so as to allow for any system of testing and used to derive an expression for the genetic advance in recurrent selection for varietal value. Covariances between relatives evaluated in the system of testing and in varietal combination are introduced, allowing a direct expression of the genetic advance in varietal development when parents are selected either individually or in groups. Some general implications for plant breeding are outlined.Dedicated to Professor F.W. Schnell on the occasion of his 65th birthday 相似文献
6.
Hoang-Tang G. H. Liang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1988,76(2):277-284
Summary The genomic relationship between cultivated sorghum [Sorghum bicolar (L.) Moench, race bicolor, De Wet, 2n=20] and Johnsongrass [S. halepense (L.) Pers., 2n=40] has been a subject of extensive studies. Nevertheless, there is no general consensus concerning the ploidy level and the number of genomes present in the two species. This research tested the validity of four major genomic models that have been proposed previously for the two species by studying chromosome behaviors in the parental species, 30-chromosome hybrids [sorghum, (2n=20) x Johnsongrass, (2n=40)], 40-chromosome hybrids [sorghum, (2n=40) x Johnsongrass, (2n=40)] and 60-chromosome amphiploids. Chromosome pairings of amphiploids are reported for the first time. Chromosomes of cultivated sorghums paired exclusively as 10 bivalents, whereas Johnsongrass had a maximum configuration of 5 ring quadrivalents with occasional hexavalents and octovalents. In contrast, 40-chromosome cultivated sorghum had up to 9 ring quadrivalents and 1 hexavalent. Pairing in the 30-chromosome hybrids showed a maximum of 10 trivalents, and that in the 40-chromosome hybrids exhibited 8 quadrivalents, 5 of which were rings, together with a few hexavalents. Amphiploid plants showed up to 3 ring hexavalents, 1 chain hexavalent and a chain of 12 chromosomes. The data suggest that cultivated sorghum is a tetraploid species with the genomic formula AAB1B1, and Johnsongrass is a segmental auto-allo-octoploid, AAAA B1B1B2B2. The model is further substantiated by chromosome pairing in amphiploid plants whose proposed genomic formula is AAAAAA B1B1B1B1 B2B2.Contribution no. 87-391-J from the Kansas Agriculatural Experiment Station 相似文献
7.
Summary The effects of food on breeding success and behavior of the red-winged blackbird (Icteridae: Agelaius phoeniceus) were investigated during 3 successive breeding seasons. In the second season, a 4-week pulse of abundant food in the form of a periodical cicada emergence (Homoptera: Cicadidae: Magicicada spp.) occurred in the forest adjacent to the marsh where the birds were breeding.During the cicada period, the bird population showed: 1) an increase in foraging trips to the forest and a decrease in trips per h, 2) increased biomass of nestlings, 3) increased nestling survival caused by decreased starvation, 4) increased fledging success, and 5) bimodal weight distributions of older nestlings (reflective of the sexual dimorphism in this species). These data suggest the temporary removal of food limitations on the breeding population when the pulse of food was available. 相似文献
8.
黄海车牛山岛白腰雨燕的繁殖习性及种群动态 总被引:1,自引:0,他引:1
本文为作者于1980—1981年对黄海车牛山岛白腰雨燕Apuspacificus pacificus (Latham)的初步研究。内容包括对繁殖习性的观察;对繁殖周期、卵孵化率、雏鸟成活串以及出生率等的统计。并对海岛生态环境下白腰雨燕种群数量的变动及其影响因素,作初步探讨。 相似文献
9.
M. Keil A. R. Griffin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(4):442-450
We carried out four separate studies using random amplified polymorphic DNA (RAPD) markers to analyse samples of Eucalyptus supplied by several different organisations. The objective was to examine the reproducibility of the RAPD technique and its ability to discriminate between individual genotypes for verification of clonal identities. We found that RAPD profiles that are unique to a genotype can be generated reliably and simply and that even closely related genotypes can be distinguished. In addition, in each of the four studies, we detected cases where the plant material studied had been mis-sampled or mis-labelled (i.e. the RAPD profiles were not consistent with the identification numbers): (1) ramets of a Eucalyptus grandis clone were found to be derived from 2 different clones; (2) ramets labelled as 2 different Eucalyptus hybrid clones were found to be the same clone, owing to a mis-planted clonal hedge; (3) samples supplied as a single progeny of a controlled E. nitens cross were derived from two crosses involving different pairs of parents; (4) mis-labelling was detected for ramets of 4 of a set of 10 clones of E. grandis and E. camaldulensis. For three of the four studies, the detection of genotype mis-identifications was unexpected, suggesting that labelling or sampling errors during the handling of plant material are a frequent occurrence, with potentially serious economic consequences. 相似文献
10.
L. E. Talbert N. K. Blake P. W. Chee T. K. Blake G. M. Magyar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,87(7):789-794
The polymerase chain reaction (PCR) is an attractive technique for many genome mapping and characterization projects. One PCR approach which has been evaluated involves the use of randomly amplified polymorphic DNA (RAPD). An alternative to RAPDs is the sequence-tagged-site (STS) approach, whereby PCR primers are designed from mapped low-copy-number sequences. In this study, we sequenced and designed primers from 22 wheat RFLP clones in addition to testing 15 primer sets that had been previously used to amplify DNA sequences in the barley genome. Our results indicated that most of the primers amplified sequences that mapped to the expected chromosomes in wheat. Additionally, 9 of 16 primer sets tested revealed polymorphisms among 20 hexaploid wheat genotypes when PCR products were digested with restriction enzymes. These results suggest that the STS-based PCR analysis will be useful for generation of informative molecular markers in hexaploid wheat.Contribution no. J-2833 of the Montana Agric Exp Stn 相似文献