鳜鱼外周血细胞显微和亚显微结构的观察

本文报道了鳜鱼外周血细胞的显微和亚显微结构。血涂片经过染色，可鉴别出红细胞、血栓细胞、淋巴细胞、单核细胞和嗜中性粒细胞；还见到幼稚的正在分裂的红细胞，提示红细胞亦可在外周血液中通过直接分裂产生。白细胞中，血栓细胞体积最小，嗜中性粒细胞体积最大；单核细胞数目最少，血栓细胞数目最多。电镜下，红细胞中可见线粒体和高尔基复合体；淋巴细胞线粒体中可见类似髓样体的板层状结构；血栓细胞和单核细胞与其它鱼类的基本  

In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells

In addition to long-term self-renewal capability, human mesenchymal stem cells (MSCs) possess versatile differentiation potential ranging from mesenchyme-related multipotency to neuroectodermal and endodermal competency. Of particular concern is hepatogenic potential that can be used for liver-directed stem cell therapy and transplantation. In this study, we have investigated whether human umbilical cord blood (UCB)-derived MSCs are also able to differentiate into hepatocyte-like cells. MSCs isolated from UCB were cultured under the pro-hepatogenic condition similar to that for bone marrow (BM)-derived MSCs. Expression of a variety of hepatic lineage markers was analyzed by flow cytometry, RT-PCR, Western blot, and immunofluorescence. The functionality of differentiated cells was assessed by their ability to incorporate DiI-acetylated low-density lipoprotein (DiI-Ac-LDL). As the cells were morphologically transformed into hepatocyte-like cells, they expressed Thy-1, c-Kit, and Flt-3 at the cell surface, as well as albumin, alpha-fetoprotein, and cytokeratin-18 and 19 in the interior. Moreover, about a half of the cells were found to acquire the capability to transport DiI-Ac-LDL. Based on these observations, and taking into account immense advantages of UCB over other stem cell sources, we conclude that UCB-derived MSCs retain hepatogenic potential suitable for cell therapy and transplantation against intractable liver diseases.  

云南血竭的化学成分及抗真菌活性

云南血竭为剑叶龙血树（Ｄｒａｃａｅｎａ ｃｏｃｈｉｎｃｈｉｎｅｎｓｉｓ（Ｌｏｕｒ．）Ｓ．ｃ．Ｃｈｅｎ）树脂，从中分离到５个芳香族化合物：对羟基苯甲酸乙酰（１），７，４＾′－二羟基黄烷（２），７－羟基－４＾′－甲氧基黄烷（３），７，４＾′－二羟基黄酮（４）和ｌｏｕｒｅｉｒｉｎ Ａ（５）以及１个甾体皂甙（６），并对其中３个酚性成分进行了抗真菌活性检测。另外，用薄层层析法对云南血竭、广西血竭、海南血竭及  

二龄鲢和鳙血液的比较研究

以１１５尾二龄链和９１尾二龄鳙为材料，测定了血液中红细胞数，白细胞数、血红蛋白值、红细胞脆性植、比积及比重，红细胞沉降率，白细胞分数计数值及各型血细胞大小等正常值。结果显示：红细胞和白细胞值，比积值及淋巴细胞所占百分比值均是鲢高于鳙，而红细胞沉降率和中性粒细胞却是鳙高于链，在鲢上，其血液指标植也存在雌雄差异，上述血液指标值也存在周年变化。  

草鱼血液的研究

本文报道了212尾池养草鱼Ctenopharyngodon idellus的血液中红细胞数、自细胞数、血红蛋白值、红细胞脆性值、比积及比重值、血细胞沉降率、白细胞分类计数和各型血细胞体的大小等正常数值和上述常数值在雌雄个体上的差异,以及鱼体年龄和繁殖季节产卵前后的差异等,为草鱼血液的常数值提供了较为系统的资料。  

草鱼肾脏和脾脏血细胞发育过程的观察

鱼类肾脏、脾脏是机体造血的主要器官。印迹涂片显示:草鱼肾脏和脾脏内血细胞的发育过程大致经历了三个阶段,即原始阶段、幼稚阶段、成熟阶段。本文着重描述了各阶段细胞的形态特征井对草鱼血细胞的发育及命名等问题作了初步的探讨。  

蛋鸡品系RAPD变异及其与杂种优势关系的分析

为探讨RAPD标记在家鸡杂种优势预测中的应用价值，分析了力康自壳蛋鸡纯系的RAPD变异及其与杂种优势的关系．5个纯系内和系间显示出低水平的变异．根据品系间遗传距离DS用UPGMA法进行聚类分析，品系a、e、d聚为一类，而h、l聚为另一类．简单线性相关分析表明，父系相似系数与F1代的60周龄产蛋数存在正相关而与蛋重的杂种优势率存在负相关；母系相似系数与产蛋数的杂种优势率存在高度负相关．但品系间遗传距离与各性状及其杂种优势率之间没有显著的相关关系．  

Mesenchymal stem cells from cryopreserved human umbilical cord blood

Umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, but the presence of mesenchymal stem cells (MSCs) in UCB has been disputed and it remains to be validated. In this study, we examined the ability of cryopreserved UCB harvests to produce cells with characteristics of MSCs. We were able to obtain homogeneous plastic adherent cells from the mononuclear cell fractions of cryopreserved UCB using our culture conditions. These adherent cell populations exhibited fibroblast-like morphology and typical mesenchymal-like immunophenotypes (CD73+, CD105+, and CD166+, etc.). These cells presented the self-renewal capacity and the mesenchymal cell-lineage potential to form bone, fat, and cartilage. Moreover, they expressed mRNAs of multi-lineage genes including SDF-1, NeuroD, and VEGF-R1, suggesting that the obtained cells had the multi-differentiation capacity as bone marrow-derived MSCs. These results indicate that cryopreserved human UCB fractions can be used as an alternative source of MSCs for experimental and therapeutic applications.  

多因素复合制作气虚血瘀证脑缺血动物模型的实验研究

目的通过复制气虚血瘀证型大鼠脑缺血动物模型,探索病证结合动物模型制作方法.方法选用老年Wistar大鼠,采用饥饿、疲劳、寒湿、惊恐、高脂饮食等多因素复合方法复制气虚血瘀证动物模型,采用双侧颈总动脉结扎复制脑缺血动物模型.结果通过对一般体征和微观指标的观测,发现模型大鼠基本符合中医气虚血瘀证候特点和现代医学脑缺血病理变化规律.[HTH〗结论多因素复合作用可成功复制病证结合动物模型,本动物模型有可能成为将来中医药防治脑缺血科研工作的实验基础.  

军曹鱼血液指标及血细胞发生的观察

测定军曹鱼的血液指标,红细胞密度为2．69±0．86×106个/mm3,白细胞密度为1．50±0．09×104个/mm3;血 红蛋白含量为7．42±0．22g/L,红细胞渗透脆性为0．43±0．07g％,红细胞沉降速率为1．18±0．46mm/h。观察军曹鱼 外周血液涂片,可区分出红细胞、血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞,但没有发现嗜酸性粒细胞和嗜碱 性粒细胞。在外周血液涂片观察中还发现了较多未成熟的红细胞和嗜中性粒细胞以及少量正在分裂的红细胞。 血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞在白细胞中所占比例分别为61．20±6．30％,16．60±3．28％,16．00± 3．61％和6．20±3．90％。对肝脏、脾脏、头肾和中肾等四种造血组织进行了涂片观察,军曹鱼的血细胞主要在头肾 和肾脏产生;脾脏是军曹鱼粒细胞发生的另一个场所,而肝脏也具有产生粒细胞和淋巴细胞的作用。