全文获取类型
收费全文 | 121篇 |
免费 | 5篇 |
国内免费 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 1篇 |
2014年 | 11篇 |
2013年 | 3篇 |
2012年 | 5篇 |
2011年 | 12篇 |
2010年 | 2篇 |
2009年 | 15篇 |
2008年 | 17篇 |
2007年 | 20篇 |
2006年 | 5篇 |
2005年 | 2篇 |
2004年 | 7篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 1篇 |
排序方式: 共有139条查询结果,搜索用时 562 毫秒
1.
Michele I. Van Dyke Susan L. Gulley Hung Lee Jack T. Trevors 《Journal of industrial microbiology & biotechnology》1993,11(3):163-170
Summary Several microbially produced biosurfactants were evaluated for their ability to remove hydrophobic compounds from soil. The biosurfactants produced byPseudomonas aeruginosa UG2 andAcinetobacter calcoaceticus RAG-1 displayed the best results, with recovery of [14C]hexachlorobiphenyl from soil slurries of 48.0 and 41.9%, respectively.P. aeruginosa UG2 produced higher levels of extracellular biosurfactants than four otherP. aeruginosa strains.P. aeruginosa UG2 culture filtrate containing biosurfactants enhanced the recovery of several other individual hydrocarbons and polychlorinated biphenyl compounds, as well as several hydrocarbons in a mixture, from soil. The results, suggest that biosurfactants produced byP. aeruginosa UG2 have the potential for remediation of hydrophobic pollutants in soil environments. 相似文献
2.
K. Petrikov Ya. Delegan A. Surin O. Ponamoreva I. Puntus A. Filonov A. Boronin 《Process Biochemistry》2013,48(5-6):931-935
We studied formation and structural features of biosurfactants produced by five oil-degrading Pseudomonas and Rhodococcus strains. These bacteria were found to be capable of intense formation of extracellular glycolipid biosurfactants when grown on mineral salts medium with 2% hexadecane. Under these conditions, the surface tension of the cultures decreased from 77 mN/m to 31–34 mN/m. The strain Rhodococcus sp. S26 forming up to 780 mg glycolipids/l of culture medium proved the most efficient biosurfactant producer. Extracellular glycolipids were purified from the crude extracts by column chromatography. Their structural features were determined by thin layer chromatography and electrospray ionization mass spectrometry. Strains Pseudomonas putida BS3701 and Pseudomonas fluorescens 142NF synthesized a number of glycolipids identified as rhamnolipid B and its homologues. Glycolipids produced by Rhodococcus sp. X5 and Rhodococcus sp. S26 were assigned to trehalose tetraesters. 相似文献
3.
Production of a lipopeptide surfactant, surfactin, by Bacillus subtilis ATCC 21332 was highly enhanced when iron concentration in the medium was raised from 4 μ
to the few m
level. This iron enrichment of the medium also resulted in increased biomass concentration. With 1.7 m
iron sulfate added to the media of different initial iron concentrations or at different stages of growth, surfactin concentration can be raised to levels of several hundred mg l−1 in general. Acidification of the broth was usually observed following the iron addition, and surfactin in the broth disappeared rapidly due to precipitate as soon as the pH level dropped below 5.0; however, if this acidification phenomenon was delayed, avoided (for reasons still unknown), or reversed (by alkaline addition), surfactin production was highly enhanced to as high as 3,500 mg l−1 which was almost ten times over the previously reported level for this strain and higher than most reported values for genetically improved strains. 相似文献
4.
Folding and stability of bacterial outer membrane proteins (OMPs) are typically studied in vitro using model systems such as phospholipid vesicles or surfactant. OMP folding requires surfactant concentrations above the critical micelle concentration (cmc) and usually only occurs in neutral or zwitterionic surfactants, but not in anionic or cationic surfactants. Various Gram-negative bacteria produce the anionic biosurfactant rhamnolipid. Here we show that the OMP OmpA can be folded in rhamnolipid at concentrations above the cmc, though the thermal stability is reduced compared to the non-ionic surfactant dodecyl maltoside. We discuss implications for possible interactions between OMPs and biosurfactants in vivo. 相似文献
5.
Imura T Ito S Azumi R Yanagishita H Sakai H Abe M Kitamoto D 《Biotechnology letters》2007,29(6):865-870
A carbohydrate ligand system has been developed which is composed of self-assembled monolayers (SAMs) of mannosylerythritol
lipid-A (MEL-A) from Pseudozyma antarctica, serving for human immunoglobulin G and M (HIgG and HIgM). The estimated binding constants from surface plasmon resonance
(SPR) measurement were K
a = 9.4 × 106 M−1 for HIgG and 5.4 × 106 M−1 for HIgM, respectively. The binding site was not in the Fc region of immunoglobulin but in the Fab region. Large amounts
of HIgG and HIgM bound to MEL-A SAMs were directly observed by atomic force microscopy. 相似文献
6.
The application of a high throughput analysis method for the screening of potential biosurfactants from natural sources 总被引:2,自引:0,他引:2
The presence of biosurfactants in growth media can be evaluated by a variety of methods, none of which are suitable for high throughput studies. The method described here is based on the effect of meniscus shape on the image of a grid viewed through the wells of a 96-well plate. The efficacy of the method was demonstrated by the selection of a bacterium (producing a biosurfactant able to reduce the surface tension of pure water from 72 to 28.75 mN m− 1) from a culture collection isolated from aviation fuel-contaminated land. The assay was found to be more sensitive, rapid and easy to perform than other published methods. It does not need specialised equipment or chemicals and excludes the bias which results from the surfactant properties of medium used for bacterial growth. 相似文献
7.
Van Bogaert IN Saerens K De Muynck C Develter D Soetaert W Vandamme EJ 《Applied microbiology and biotechnology》2007,76(1):23-34
Sophorolipids are surface-active compounds synthesized by a selected number of yeast species. They have been known for over
40 years, but because of growing environmental awareness, they recently regained attention as biosurfactants due to their
biodegradability, low ecotoxicity, and production based on renewable resources. In this paper, an overview is given of the
producing yeast strains and various aspects of fermentative sophorolipid production. Also, the biochemical pathways and regulatory
mechanisms involved in sophorolipid biosynthesis are outlined. To conclude, a summary is given on possible applications of
sophorolipids, either as native or modified molecules. 相似文献
8.
Francisco J. Aranda María J. Espuny Ángeles Manresa Antonio Ortiz 《生物化学与生物物理学报:生物膜》2007,1768(10):2596-2604
The study of the interaction of biosurfactants with biological membranes is of great interest in order to gain insight into the molecular mechanisms of their biological actions. In this work we report on the interaction of a bacterial trehalose lipid produced by Rhodococcus sp. with phosphatidylcholine membranes. Differential scanning calorimetry measurements show a good miscibility of the glycolipid in the gel state and immiscibility in the fluid state, suggesting domain formation. These domains have been visualized and characterized, for the first time, by scanning force microscopy. Incorporation of trehalose lipid into phosphatidylcholine membranes produces a small shift of the antisymmetric stretching band toward higher wavenumbers, as shown by FTIR, which indicates a weak increase in fluidity. The CO stretching band shows that incorporation of trehalose lipid increases the proportion of the dehydrated component in mixtures with the three phospholipids at temperatures below and above the gel to liquid-crystalline phase transition. This dehydration effect is also supported by data on the phospholipid PO stretching bands. Small-angle X-ray diffraction measurements show that in the samples containing trehalose lipid the interlamellar repeat distance is larger than in those of pure phospholipids. These results are discussed within the frame of trehalose lipid domain formation, trehalose lipid/phospholipid interactions and its relevance to membrane-related biological actions. 相似文献
9.
Manoj Kumar Vladimir León Angela De Sisto Materano Olaf A. Ilzins 《World journal of microbiology & biotechnology》2007,23(2):211-220
A Bacillus sp. strain DHT, isolated from oil-contaminated soil, grew and produced biosurfactant when cultured in variety of substrate
at salinities of up to 100 g l−1 and temperatures up to 45°C. It was capable of utilizing crude oil, fuels, various pure alkanes and PAHs as a sole carbon
and energy source across a wide range of temperature and salinity. Over the range evaluated, the degradation of hydrocarbon
and biosurfactant production was not influenced by salinity (0–10% wv−1) and temperature (30–45°C). The biosurfactant produced by the organism emulsified a range of hydrocarbons with hexadecane
as the best substrate and toluene as the poorest. From 16S rDNA analysis, strain DHT was related to Bacillus licheniformis. 相似文献
10.
Grażyna A. Płaza Jacek Wypych Christopher Berry Robin L. Brigmon 《World journal of microbiology & biotechnology》2007,23(4):533-542
The fate of benzene, ethylbenzene, toluene, xylenes (BTEX) compounds through biodegradation was investigated using two different
bacteria, Ralstonia picketti (BP-20) and Alcaligenes piechaudii (CZOR L-1B). These bacteria were isolated from extremely polluted soils contaminated with petroleum hydrocarbons. PCR and
Fatty Acid Methyl Ester (FAME) were used to identify the isolates. In this study, BTEX biodegradation, applied as a mixture
or as individual compounds by the bacteria was evaluated. Both bacteria were shown to degrade each of the BTEX compounds individually
and in mixture. However, Alcaligenes piechaudii was a better degrader of BTEXs both in the mixture and individually. Differences between BTEX biodegradation in the mixture
and individually were observed, especially in the case of benzene. The degradation of all BTEXs in the mixture was lower than
the degradation of individual compounds for both bacteria tested. In the all experiments, toluene and m + p- xylenes were better removed than the other BTEXs. No intermediates of biodegradation were detected. Biosurfactant production
was observed by culture techniques. In addition, 3-hydroxy fatty acids, important in biosurfactant production, were observed
by FAME analysis. The test results indicate that the bacteria could contribute to bioremediation of aromatic hydrocarbon pollution. 相似文献