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1.
Periela da Silva Sousa-Vasconcelos Wellington da Silva Seguins Eduardo de Souza Luz Rosa Teixeira de Pinho 《Memórias do Instituto Oswaldo Cruz》2015,110(6):809-813
Tuberculosis has great public health impact with high rates of mortality and the only
prophylactic measure for it is the Mycobacterium bovisbacillus
Calmette-Guérin (BCG) vaccine. The present study evaluated the release of cytokines
[interleukin (IL)-1, tumour necrosis factor and IL-6] and chemokines [macrophage
inflammatory protein (MIP)-1α and MIP-1β] by THP-1 derived
macrophages infected with BCG vaccine obtained by growing mycobacteria in Viscondessa
de Moraes Institute medium medium (oral) or Sauton medium (intradermic) to compare
the effects of live and heat-killed (HK) mycobacteria. Because BCG has been reported
to lose viability during the lyophilisation process and during storage, we examined
whether exposing BCG to different temperatures also triggers differences in the
expression of some important cytokines and chemokines of the immune response.
Interestingly, we observed that HK mycobacteria stimulated cytokine and chemokine
production in a different pattern from that observed with live mycobacteria. 相似文献
2.
Early‐shared Mycobacterium bovis bacillus Calmette–Guérin sub‐strains induce Th1 cytokine production in vivo 下载免费PDF全文
Keiichi Taniguchi Yuuji Miyatake Daisuke Hayashi Atsuro Takami Saotomo Itoh Saburo Yamamoto Shigeaki Hida Kikuo Onozaki Takemasa Takii 《Microbiology and immunology》2015,59(11):684-689
3.
Tuberculosis (TB) is one of the world’s leading causes of death due to infection and efforts to control TB would be substantially
aided by the availability of an improved TB vaccine. There are currently nine new TB vaccines in clinical development, and
the first efficacy trials are due to commence in 2009. There are many complex ethical issues which arise at all stages of
TB vaccine development, from the need to conduct trials in developing countries to informed consent and the process of ethical
review. While it is important that these issues are discussed, it may also be timely to consider the challenges which may
arise if a vaccine in clinical development proves to be highly effective. We examine a number of scenarios where decisions
on the deployment of a new TB vaccine may impact on the rights and liberty of the individual.
Competing Interest Statement Helen McShane is an inventor on a composition of matter patent and a shareholder in a Joint Venture, Oxford-Emergent Tuberculosis
Consortium, formed to develop MVA85A. The views expressed in this article are those of the author(s) and do not necessarily
represent those of the Oxford-Emergent Tuberculosis Consortium Ltd 相似文献
4.
Masahiko Makino Yumi Maeda Masanori Kai Toshiki Tamura & Tetsu Mukai 《FEMS immunology and medical microbiology》2009,55(1):39-46
The potential of Mycobacterium bovis Bacillus Calmette–Guerin (BCG) needs to be augmented to efficiently activate CD4+ T cells through macrophages. Mycobacterium leprae -derived recombinant major membrane protein (MMP)-II induced GM-CSF production from macrophages. A recombinant BCG-SM that secretes MMP-II more efficiently produced GM-CSF and activated interferon (IFN)-γ-producing CD4+ T cells than did vector control BCG when infected with macrophages. The T-cell activation by BCG-SM was dependent on the GM-CSF production by macrophages. Interleukin (IL)-10 production by macrophages stimulated with M. leprae was inhibited in a GM-CSF-dependent manner when the precursor monocytes were infected with BCG-SM. BCG inducing GM-CSF production was effective in macrophage-mediated T-cell activation partially through IL-10 inhibition. 相似文献
5.
6.
目的:观察卡介苗(BCG)单独作用膀胱肿瘤细胞、正常膀胱移行上皮细胞及其代谢产物作用上述细胞后细胞生长情况及各自细胞培养液上清液中细胞因子(TNF-α.、IL-10、IFN-γ)浓度的变化,探讨其在卡介苗治疗膀胱肿瘤中可能的作用机制。方法:构建大鼠膀胱肿瘤模型,并原代培养大鼠膀胱肿瘤细胞及正常膀胱移行上皮细胞。分别用BCG,普通培养液和细胞培养的代谢产物作用上述细胞。酶联接免疫吸附剂测定法(ELISA法)检测各组细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、干扰素-γ(IFN-γ)的浓度。结果:ELISA法检测各组细胞上清液中TNF-α、IL-10的浓度改变有显著差异,而IFN-γ的浓度无显著差异。结论:BCG可以直接刺激肿瘤细胞自身分泌细胞因子(TNF-α、IL-10)参与调节抑制肿瘤细胞的生长。 相似文献
7.
Marian L. Kruzel Jeffrey K. Actor Michał Zimecki Jasen Wise Paulina Płoszaj Shaper Mirza Mark Kruzel Shen-An Hwang Xueqing Ba Istvan Boldogh 《Journal of biotechnology》2013
Lactoferrin, an iron-binding protein found in high concentrations in mammalian exocrine secretions, is an important component of the host defense system. It is also a major protein of the secondary granules of neutrophils from which is released upon activation. Due to its potential clinical utility, recombinant human lactoferrin (rhLF) has been produced in various eukaryotic expression systems; however, none of these are fully compatible with humans. Most of the biopharmaceuticals approved by the FDA for use in humans are produced in mammalian expression systems. The Chinese hamster ovary cells (CHO) have become the system of choice for proteins that require post-translational modifications, such as glycoproteins. 相似文献
8.
BCG-CpG-DNA制备、理化特性及免疫刺激活性的初步研究 总被引:9,自引:1,他引:8
采用机械破碎、CTAB沉淀、酚:氯仿:异戊醇抽提法从卡介菌 (BCG)中制备BCG- CpG- DNA,经化学方法确定其理化成分,通过双抗体夹心ELISA法检测小鼠IgM水平检测其免疫刺激活性。结果显示每克半干重的卡介菌提取BCG- CpG -DNA约 0. 9mg。提取物主要成分为BCG -CpG -DNA,分子量大小在 3 000~15 710bp,含少量的多糖和蛋白;紫外分光扫描在 260nm有最大吸收;对特异性识别DNA长链的CCGG中的CpG位点的限制性内切酶HpaⅡ高度敏感;能够活化小鼠B细胞产生IgM。所制备的BCG -CpG- DNA含较多的未甲基化CpG基序,具有免疫刺激活性功能。 相似文献
9.
10.
《Microbes and infection / Institut Pasteur》2014,16(1):73-79
A better understanding of mucosal immunity is required to develop more protective vaccines against Mycobacterium tuberculosis. We developed a murine aerosol challenge model to investigate responses capable of protecting against mucosal infection. Mice received vaccinations intranasally with CpG-adjuvanted antigen 85B (Ag85B/CpG) and/or Bacillus Calmette–Guerin (BCG). Protection against aerosol challenge with a recombinant GFP-expressing BCG was assessed. Mucosal prime/boost vaccinations with Ag85B/CpG and BCG were protective, but did not prevent lung infection indicating more efficacious mucosal vaccines are needed. Our novel finding that protection correlated with increased airway dendritic cells early post-challenge could help guide the development of enhanced mucosal vaccines. 相似文献