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1.
目的:探讨自拟消水散联合利尿剂治疗癌性腹水的临床疗效。方法:选取恶性肿瘤伴癌性腹水患者84例,按随机数字表法分组,分别为42例,对照组予以常规利尿剂治疗,研究组予以自拟消水散联合利尿剂治疗。观察并比较两组患者治疗前后血清TNF-α,IL-2,IL-4,IL-6,CD3~+,CD4~+及CD4~+/CD8~+含量的变化情况以及临床疗效。结果:与治疗前对比,两组治疗后血清TNF-α,IL-2,IL-4及IL-6均降低,CD3~+,CD4~+及CD4~+/CD8~+均升高,CD8~+均降低,CEA,CA125,CA153及CA199均降低(P0.05);与对照组对比,研究组治疗后血清TNF-α,IL-2,IL-4,IL-6较低,CD3~+,CD4~+及CD4~+/CD8~+较高,CD8~+较低,CEA,CA125,CA153及CA199较低(P0.05);研究组治疗有效率高于对照组,差异具有统计学意义(P0.05)。结论:消水散联合利尿剂治疗癌性腹水的疗效确切,能显著降低炎症指标及肿瘤标志物,提高机体免疫功能。 相似文献
2.
目的:对分离自新疆沙漠的4株小球藻GTD8A1、GTD4A-1、GTD7C-2和TLD6B进行几种常用基因工程抗生素的敏感性研究,以期筛选出沙漠小球藻基因工程中选择标记的抗生素。方法:运用藻株在液体培养过程中藻体颜色变化和血球板细胞计数的方法,系统性研究沙漠小球藻对几种常用基因工程抗生素的敏感性。结果:4株沙漠小球藻对卡那霉素和链霉素都非常敏感,敏感浓度(细胞致死浓度,下同)均为25μg/m L;对氯霉素也很敏感,GTD8A1、GTD7C-2和TLD6B的敏感浓度也均为25μg/m L,GTD4A-1的敏感浓度为100μg/m L;4株沙漠小球藻对氨苄西林和头孢霉素的敏感性都不是很明显,在一定的浓度范围,氨苄西林和头孢霉素对藻细胞的生长还具有促进作用,当氨苄西林和头孢霉素的浓度很高时才表现出一定的抑制作用。结论:卡那霉素和链霉素可作为沙漠小球藻基因工程选择标记中的2种抗生素,为今后建立其遗传转化系统奠定了基础。 相似文献
3.
Giardia lamblia is a unicellular, early branching eukaryote causing giardiasis, one of the most common human enteric diseases. Giardia, a microaerophilic protozoan parasite has to build up mechanisms to protect themselves against oxidative stress within the human gut (oxygen concentration 60 μM) to establish its pathogenesis. G. lamblia is devoid of the conventional mechanisms of the oxidative stress management system, including superoxide dismutase, catalase, peroxidase, and glutathione cycling, which are present in most eukaryotes. NADH oxidase is a major component of the electron transport chain of G. lamblia, which in concurrence with disulfide reductase, protects oxygen-labile proteins such as pyruvate: ferredoxin oxidoreductase against oxidative stress by sustaining a reduced intracellular environment. It also contains the arginine dihydrolase pathway, which occurs in a number of anaerobic prokaryotes, includes substrate level phosphorylation and adequately active to make a major contribution to ATP production. 相似文献
4.
玉米根ABA结合蛋白的亚细胞定位及动力学性质 总被引:9,自引:0,他引:9
以玉米(Zea maysL.)根或胚芽鞘为材料,经匀浆、分级离心得到胞质部分和膜部分(微粒体),进一步用6.2% (W/W ) Dextran T500 和PEG 3350 两相系统制备质膜,用1% 和8% (W /W) Dextran T70 梯度离心制备液泡膜. 电镜鉴定及多种标志酶检测表明,制备获得了高纯度正向型质膜和富含液泡膜的组分,其它内膜的污染很少. 用微量放射配体结合(MRLB)实验证明,玉米根微粒体的ABA专一性结合位点主要分布在液泡膜和质膜上,这两种膜组分与ABA 的特异结合活性分别为2485.4 fm ol/m g protein 和1257.3 fm ol/m g pro-tein,玉米根段胞质部分结合活性最低(差一个数量级).质膜上ABA-BP与ABA 的结合平衡解离常数(KD)为1.57 nm ol/L. 相似文献
5.
Melisa A. Conde Natalia P. Alza Pablo A. Iglesias González Paola G. Scodelaro Bilbao Sofía Sánchez Campos Romina M. Uranga Gabriela A. Salvador 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2018,1863(6):639-650
We have previously shown that phospholipase D (PLD) pathways have a role in neuronal degeneration; in particular, we found that PLD activation is associated with synaptic injury induced by oxidative stress. In the present study, we investigated the effect of α-synuclein (α-syn) overexpression on PLD signaling. Wild Type (WT) α-syn was found to trigger the inhibition of PLD1 expression as well as a decrease in ERK1/2 phosphorylation and expression levels. Moreover, ERK1/2 subcellular localization was shown to be modulated by WT α-syn in a PLD1-dependent manner. Indeed, PLD1 inhibition was found to alter the neurofilament network and F-actin distribution regardless of the presence of WT α-syn. In line with this, neuroblastoma cells expressing WT α-syn exhibited a degenerative-like phenotype characterized by a marked reduction in neurofilament light subunit (NFL) expression and the rearrangement of the F-actin organization, compared with either the untransfected or the empty vector-transfected cells. The gain of function of PLD1 through the overexpression of its active form had the effect of restoring NFL expression in WT α-syn neurons. Taken together, our findings reveal an unforeseen role for α-syn in PLD regulation: PLD1 downregulation may constitute an early mechanism in the initial stages of WT α-syn-triggered neurodegeneration. 相似文献
6.
《Current biology : CB》2020,30(20):4033-4046.e8
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7.
Hucho taimen are listed as endangered in China. The population size has declined recently, prompting an increase in the level of listing from grade three in 2002 to grade five in 2006. We analyzed the genetic diversity of wild populations using 17 microsatellite markers to establish a scientific basis for conservation of this species. We collected tissue samples from four populations in the Heilongjiang River basin: Huma River (HM), Hutou (HT), Haiqing (HQ), and Zhuaji (ZJ). A total of 21 loci were amplified, 18 of which were polymorphic. The number of alleles per locus ranged from 2 to 9 (mean: 4.1905). There were 13 highly polymorphic loci and 5 moderately polymorphic loci. Analysis of five genetic diversity parameters (Na, Ne, Ho, He, and PIC) suggested moderate levels of diversity within the populations. The populations were ranked HT > HQ > ZJ > HM, but the differences in diversity were not statistically significant (P > 0.05). A comparison of variation among all four populations suggested Hardy–Weinberg disequilibrium at 20% of the loci. Genetic differentiation (Fst) was 0.0644 and the gene flow among populations was estimated at 3.36 individuals per generation. The majority of diversity (93.88%) occurred among individuals within a population. In contrast, relatively little (6.12%) of the genetic diversity was distributed between the populations. An analysis of genetic differentiation and genetic distance between pairs of populations revealed that both parameters were higher in comparisons of the HM population to the HT, HQ, and ZJ populations than among the three latter populations. This suggests that the HM population has a distinct genetic structure. We hypothesize that habitat degradation and excessive fishing, not low genetic diversity, has caused the decline in H. taimen populations. However, this species should be protected from further declines in genetic diversity. 相似文献
8.
Huaan Yang Daniel Renshaw Geoff Thomas Bevan Buirchell Mark Sweetingham 《Molecular breeding : new strategies in plant improvement》2008,21(4):473-483
A key challenge in marker-assisted selection (MAS) for molecular plant breeding is to develop markers linked to genes of interest
which are applicable to multiple breeding populations. In this study representative F2 plants from a cross Mandalup (resistant to anthracnose disease) × Quilinock (susceptible) of Lupinus angustifolius were used in DNA fingerprinting by Microsatellite-anchored Fragment Length Polymorphism (MFLP). Nine candidate MFLP markers
linked to anthracnose resistance were identified, then ‘validated’ on 17 commercial cultivars. The number of “false positives”
(showing resistant-allele band but lack of the R gene) for each of the nine candidate MFLP markers on the 17 cultivars ranged
from 1 to 9. The candidate marker with least number of false positive was selected, sequenced, and was converted into a co-dominant,
sequence-specific, simple PCR based marker suitable for routine implementation. Testing on 180 F2 plants confirmed that the converted marker was linked to the R gene at 5.1 centiMorgan. The banding pattern of the converted
marker was consistent with the disease phenotype on 23 out of the 24 cultivars. This marker, designated “AnManM1”, is now
being used for MAS in the Australian lupin breeding program. We conclude that generation of multiple candidate markers, followed
by a validation step to select the best marker before conversion to an implementable form is an efficient strategy to ensure
wide applicability for MAS. 相似文献
9.
There are increasing scientific evidences suggesting that E-cadherin gene promoter hypermethylation may contribute to the development and progression of bladder cancer, but existing studies have yielded inconclusive results. This meta-analysis aims to assess the role of E-cadherin promoter hypermethylation in bladder carcinogenesis. We conducted an extensive literature search for relevant studies on PubMed, Embase, Web of Science, Cochrane Library, and CBM databases from their inception through May 1st, 2013. This meta-analysis was performed using the STATA 12.0 software. Crude risk ratio (RR) with 95% confidence interval (CI) was calculated. Ten clinical studies were included in this meta-analysis with a total of 620 bladder cancer samples, 199 normal adjacent samples and 131 normal urothelium tissue. Our meta-analysis revealed that the methylation frequencies in bladder cancer tissues were obviously higher than those in normal control tissues (RR = 2.02, 95%CI: 1.00–4.12, P = 0.050). Subgroup analysis by ethnicity indicated that higher methylation frequencies were observed in bladder cancer tissues among Asian populations (RR = 2.35, 95%CI: 1.11–4.95, P = 0.025), but not among Caucasian populations (RR = 1.62, 95%CI: 0.48–5.53, P = 0.439). Univariate and multivariate meta-regression analyses showed that ethnicity may be the major source of heterogeneity (P < 0.05). No publication bias was detected in this meta-analysis (P = 0.358). The present meta-analysis indicates that E-cadherin gene promoter hypermethylation may contribute to increased risk of bladder cancer among Asian populations. 相似文献
10.