蝘蜓消化道5-羟色胺细胞的免疫组织化学定位

应用5-羟色胺(5-Hydroxytryptamine 5-HT)特异性抗血清对蝘蜓(Sphenomorphus iudieus)消化道5-HT细胞的分布及形态进行了免疫组织化学研究。结果表明，5-HT阳性细胞在堰蜓消化道各段均有分布，其中以胃幽门部位分布密度最高，食道与直肠部位其次，空肠部位分布密度最低。消化道各段5-HT细胞形态多样，有圆形、椭圆形、梭形、楔形、不规则形，其中梭形细胞多具有胞突。文中对堰蜓消化道5-HT细胞的分布、形态与功能相适应的特点进行了讨论。  

两栖类消化道嗜银细胞和5-羟色胺细胞的研究进展

嗜银细胞属于内分泌细胞类，是消化道所含各类内分泌细胞的总称，5-羟色胺是其中含量和分布范围较广的一种胃肠激素。本文总结了两栖动物消化道嗜银细胞和5-羟色胺细胞的形态学特征、分布位置和分布密度规律，概述了冬眠、禁食和不同生活史阶段与两种细胞分布密度的关系。  

禁食对东方蝾螈胃肠道5-羟色胺细胞形态和分布型的影响

目的 禁食对东方嵘螈(Cynops orientalis)胃肠道5-羟色胺细胞形态学特点和分布密度的影响.方法 SP(streptavidin peroxidase)免疫组织化学方法.结果 喂食组东方蝾螈胃肠道5-羟色胺开放型和闭合型细胞的比值高峰为幽门和十二指肠,直肠其次,其余部位较为接近;禁食组幽门最高,十二指肠其次,贲门、空肠、回肠和直肠最低.禁食组胃体和空肠的比值高于喂食组,回肠低于喂食组,其余部位无组间差异.喂食组幽门和直肠的分布密度最高,胃体最低;禁食后,十二指肠的分布密度最高,贲门和回肠最低.禁食组胃体的分布密度显著高于喂食组,其余部位无组间差异.结论 东方蝾螈可通过胃体、空肠和回肠开放型5-羟色胺细胞数量的调整及胃体分布密度的增加来调节禁食后胃肠道的消化和吸收能力.  

A quantitative cytochemical study of the growth of individual mast cells

Summary For individual mast cells, relationships between their dry mass and their content of heparin and 5-hydroxytryptamine (5-HT) were studied. This was achieved by measuring these parameters successively on identical cells, by means of quantitative cytochemical techniques. The peritoneal mast cells have a very long life span and a slow turnover of granule components. Increase of the dry weight of the cells may therefore be taken as an expression of cellular growth. Mast cell populations from younger and older animals were analysed in an attempt to evaluate the influence of cell-aging and animal-aging on the growth of the mast cells. The analysis was based on allometric (log-log) plots and linear regressions. Within the cell populations there were strong mutual correlations between the cell parameters studied, without any obvious deviations from linearity. However, the slopes of the allometric lines indicated a somewhat different mode of growth for mast cells from younger and older animals. The capacity of the mast cells to accumulate 5-HT after a single injection of its precursor, 5-hydroxytryptophan, was used as a functional test. In relation to the cell weight, the induced increase of 5-HT was greater for lighter than for heavier mast cells. This difference between light and heavy mast cells was greater for cells from younger than from older animals. These differences in growth and functional properties between mast cells from younger and older animals were interpreted as an effect of aging of the animals rather than of aging of the cells.Supported by grants from the Swedish Medical Research Council, Project no 2235I wish to express my sincere thanks to Professor Lennart Enerbäck for valuable and constructive criticism of this paper. I also wish to thank Lecturer Erik Leander for valuable statistical advice  

北方狭口蛙消化道5一羟色胺细胞的免疫组织化学定位

目的探讨北方狭口蛙（Kaloula borealis）消化道5-羟色胺细胞的分布密度规律和形态学特点。方法免疫组织化学SABC法鉴别和定位雌雄各6只北方狭口蛙食管、贲门、胃体、幽门、十二指肠、空肠、回肠和直肠的5-羟色胺细胞。结果5-羟色胺细胞的分布密度高峰位于胃幽门部，胃体和十二指肠其次，贲门最低。开放型和闭合型细胞的比值从食管至直肠呈”V”形分布，胃幽门部为比值的最低点。食管、贲门和肠道各段的5一羟色胺细胞以开放型细胞为主，胃体开放型和闭合型细胞的数量几近相等，胃幽门部以闭合型细胞为主。结论北方狭口蛙消化道5-羟色胺细胞的分布型和形态学特点与其他两栖动物既有相同点，也有自身的独特性，这可能与其食性和善于穴居掘土的生活方式有关。  

三种内分泌细胞在极北鲵幼体胃内的发育

采用免疫组织化学亲和素-生物素复合物(ABC)法研究极北鲵(Salamandrella keyserlingii 41至46期共6个时期幼体胃内5-羟色胺(5-HT)细胞、生长抑素(SS)细胞和胃泌素(GAS)细胞的分布位置及形态,5-羟色胺细胞和胃泌素细胞最先在41期幼体被检测出来,而生长抑素细胞发育略晚,在42期幼体才被检测到.文中采用Duncan多重比较的方法,对胃内同一种内分泌细胞不同发育时期的分布密度进行统计学分析,5-羟色胺细胞在41期分布最少,密度为1.2±0.51,46期分布最多,密度为2.3±0.91,随着胃的发育,5-羟色胺细胞密度呈递增的变化趋势;胃泌素细胞在42期分布最多,密度为2.1±0.99,46期分布次之,密度1.7±0.49,44期分布最少(密度1.0±0.00),从42期到46期呈先减后增的变化趋势;而生长抑素细胞在43期密度(2.6±0.99)高于其他各期,且差异显著(P＜0.05).三种内分泌细胞最先均出现在胃上皮细胞之间,随着胃的不断发育,除了在上皮细胞之间分布以外,在腺泡上皮之间也有分布.不同内分泌细胞随着发育时间的不同,细胞形态也表现不同,如5-羟色胺细胞由最开始的椭圆形到后期的锥体形,胃泌素细胞由最先出现的锥体形到椭圆形再到锥体形,生长抑素细胞由锥体形到椭圆形.上述三种内分泌细胞的分布及形态学特点可能与极北鲵的生长发育以及胃的功能相适应.  

花背蟾蜍消化道5-羟色胺细胞的分布及形态学特征

采用卵白素-生物素-过氧化物酶复合物（avidin-biotin-peroxidase complex technique，ABC）免疫组织化学方法对花背蟾蜍（Bufo raddei）消化道5-羟色胺（5-HT）细胞的分布密度及形态学特征进行了观察。结果显示，5-HT细胞在花背蟾蜍整个消化道中均有分布，食管、贲门、胃体和幽门的分布密度都显著高于肠道各段，胃幽门部密度最高，胃体部其次，直肠部最低。消化道各个部位5-HT开放型和闭合型细胞的比值变化范围为2.48~4.71。消化道各段均以开放型细胞为主，大多呈锥体形、梭形或不规则形，少数为闭合型细胞，呈圆形或椭圆形。花背蟾蜍5-HT细胞的形态学特征与其他两栖动物相似，但分布密度有自身特征，可能与其食性和善于摄取活动性小的食物的生活习性有关。