厚果鸡血藤的化学成分研究

继厚果鸡血藤化学成分的研究（Ⅰ）、（Ⅱ）之后,又从厚果鸡血藤Millettia pachycar pa Benth。根部分离得到多个化合物,经理化分析和波谱分析,确定了其中七个黄酮类化合物的结构为3,3′,4′5′－四甲氧基呋喃[4^∥,5^∥：8,7]黄酮（Ⅰ）,3,3′－二甲氧基呋喃[4^∥,5^∥：8,7]黄酮（Ⅱ）,2,3－二甲氧基呋喃[4′,5′：11,10]－7－氧代[2]苯吡喃[4,3－b][1]－苯吡喃（Ⅲ）,呋喃[4^∥,5^∥：8,7]黄酮（Ⅳ,Lanceolatin B),3-甲氧基－2^∥,2^∥-二甲基吡喃[5^∥,6^∥：8,7]黄酮（Ⅴ）,5－甲氧基呋喃[4^∥,5^∥：7,6]黄酮（Ⅵ,pinnatin）,2^∥,2^∥-二甲基吡喃[5^∥,6^∥：8,7]黄烷酮（Ⅷ,（-）-isolonchocarpin）。这七个化合物皆为首次从该植物中分离得到,其中化合物Ⅰ,Ⅱ,Ⅲ为新的化合物,分别命名为厚果鸡血藤丙素（pachycarin C)、厚果鸡血藤丁素（pachycarin D)和厚果鸡血藤戊素（pachycarin E）。     

大孔吸附树脂吸附分离丰城鸡血藤总黄酮的研究

通过静态吸附实验研究了AB-8和H103两种大孔吸附树脂对丰城鸡血藤总黄酮的吸附特性,筛选出适合丰城鸡血藤总黄酮分离纯化的树脂,并对其进行了动态吸附特性研究.结果表明,AB-8树脂对丰城鸡血藤总黄酮具有良好的吸附和解吸性能,经AB-8树脂吸附富集后,样品总黄酮含量由7.44%提高至40.61%,刺芒柄花素含量提高了15.7倍.因而,AB-8是良好的吸附分离丰城鸡血藤黄酮类化合物的吸附剂.     

鸡血藤化学和药理研究进展

本文对鸡血藤的化学及药理研究进展进行了综述     

安徽牯牛降的丝孢菌Ⅰ

本文报导作者采自安徽枯牛降自然保护区的18种丝孢菌,分属于5个属,其中有3个新种:牛皮冻生尾孢(Cercospora paederiicola),山鸡椒假尾饱(Pseudocercospora litseae-cubebae),鸡血藤生假尾孢(P. millettiicola)和2个中国新纪录。文中对新种进行了描述及绘图,新记录种作了简要说明。研究的标本保存在中国科学院微生物研究所真菌标本室(HMAS)。     

鸡血藤中的酚酸类化合物

为了解鸡血藤(Spatholobus suberectus Dunn)的化学成分,从鸡血藤的95%乙醇提取物中分离出15个酚酸类单体成分,经波谱学分析分别鉴定为:没食子酸(1)、tachioside(2)、isotachioside(3)、canthoside D(4)、3,5-二甲氧基-4-羟基苯基-1-O-β-D-吡喃葡萄糖苷(5)、2,6-二甲氧基-4-羟基-苯酚-1-O-β-D-吡喃葡萄糖苷(6)、4-羟甲基-2,6-二甲氧基苯基-β-D-吡喃葡萄糖苷(7)、丁香酸葡萄糖苷(8)、3-甲氧基苯乙醇-4-O-β-D-葡吡喃糖苷(9)、2-(4-hydroxy-3,5-dimethoxyphenyl)ethyl-β-D-glucopyranoside(10)、4,6-二羟基-2-O-(β-D-吡喃葡萄糖苷)苯乙酮(11)、松香(12)、顺式紫丁香苷(13)、(–)-(7R,8S)-guaiacylglycerol 8-O-β-Dglucopyranoside(14)和l-threo-guaiacylglycerol-8-O-β-glucopyranoside(15)。其中,化合物2~8和10~15为首次从密花豆属植物中分离得到。     

Zn、B配施对鸡血藤黄酮类化合物积累的影响

为探讨Zn、B配施对鸡血藤(Spatholobus suberectus)黄酮类化合物积累的影响,采用营养液叶面喷施,对其总黄酮含量、可溶性蛋白质和PAL活性的变化进行研究。结果表明,Zn、B配施的鸡血藤总黄酮含量、可溶性蛋白质含量、PAL活性增加,其中施用50 mg L–1 Zn SO4+10 mg L–1 Na_2B_4O_7·10H_2O鸡血藤的可溶性蛋白质含量最高,达0.89%;施用25 mg L–1Zn SO4+50 mg L–1 Na2B4O7·10H2O鸡血藤的总黄酮含量和PAL活性最高,分别为4.65%、29.47 U g–1min–1。因此,合理配施Zn、B能促进鸡血藤黄酮类化合物的积累。     

丰城鸡血藤黄酮提取物清除自由基活性研究

本文考察了丰城鸡血藤不同提取物对.DPPH、O2-.和.OH清除活性,分别采用紫外可见分光光度法、邻苯三酚自氧化法和Fenton反应-邻菲罗啉显色法,测定受试物对.DPPH、O2-.和.OH的清除活性。实验结果表明受试物对.DPPH清除活性较强,对O2-.的清除活性次之,对.OH的清除活性较弱。丰城鸡血藤不同提取物黄酮组成和含量存在差异,其自由基清除活性也有所不同,提示它们的自由基清除作用机制和生物活性可能存在差异。     

Effect of amino acid residue and oligosaccharide chain chemical modifications on spectral and hemagglutinating activity of Millettia dielsiana Harms. ex Diels. lectin

The effects of modifying the carbohydrate chain and amino acids on the conformation and activity of Millettia dielsiana Harms. ex Diels. lectin (MDL) were studied by hemagglutination, fluorescence and circular dichroism analysis. The modification of tryptophan residues led to a compete loss of hemagglutinating activity; however, the addition of mannose was able to prevent this loss of activity. The results indicate that two tryptophan residues are involved in the carbohydrate-binding site. Modifications of the carboxyl group residues produced an 80% loss of activity, but the presence of mannose protected against the modification. The results suggest that the carboxyl groups of aspartic and glutamic acids are involved in the carbohydrate-binding site of the lectin. However, oxidation of the carbohydrate chain and modification of the histidine and arginine residues did not affect the hemagglutinating activity of MDL. Fluorescence studies of MDL indicate that tryptophan residues are present in a relatively hydrophobic region, and the binding of mannose to MDL could quench tryptophan fluorescence without any change in λmax. The circular dichroism spectrum showed that all of these modifications affected the conformation of the MDL molecule to different extents, except the modification of arginine residues. Fluorescence quenching showed that acrylamide and iodoacetic acids are able to quench 77% and 98% of the fluorescence of tryptophan in MDL, respectively. However, KI produced a barely perceptible effect on the fluorescence of MDL, even when the concentration of I^- was 0.15M. This demonstrates that most of tryptophan residues are located in relatively hydrophobic or negatively charged areas near the surface of the MDL molecule.     

香花崖豆藤的组织培养与快速繁殖

1植物名称香花崖豆藤(Millettia dielsiana Harmsex Diels),又名大巴豆、山鸡血藤、山胡豆。2材料类别成熟种子。3培养条件基本培养基为MS。(1)种子萌发培养基:MS 6-BA0.5mg·L-1(单位下同) NAA0.5;(2)芽的分化     

中国柱隔孢菌属及其近似属的研究IV

本文报道柱隔孢菌属二新种和二个中国新记录种。新种是夹竹桃柱隔孢Ramularia nerii-indici, 寄生在夹竹桃科(Apocynaceae)夹竹桃(Nerium indicum)上;鸡血藤柱隔孢Ramularia millettiae, 寄生在豆科蝶形花亚科(Papilinoideae)昆明鸡血藤(Millettia reticulata)上。新记录种是甜菜生柱隔孢R. beticola和木香柱隔孢R. banksiana。文中为新种提供了拉丁文描述和附图。研究标本保存于云南农业大学真菌标本室(MHYAU)。