辽宁省菜田生态系统组分优势值与系统优势度分析

辽宁省菜田生态系统组分优势值与系统优势度分析周宝利，葛晓光李宁义（沈阳农业大学园艺系，１１０１６１）（沈阳市城建中专，１１００１３）ＡｎａｌｙｓｉｓｏｎＳｕｐｅｒｉｏｒｉｔｙＶａｌｕｅａｎｄＤｏｍｉｎａｎｃｅＤｅｇｒｅｅｏｆＶａｒｉｏｕｓＣｏｍｐｏｎ...     

建立以TK基因为标记将外源基因导入臭曲霉（Asper—gillus...

A new system for selection of transformed Aspergillus foetidus was reported. In this system, TK- A. foetidus which were constructed by homologous recombination of mutated TK gene of vaccinia virus with TK gene of A. foetidus were screened by adding BUdR in agar plates. Conditions for screen of TK+ A. foetidus strain, transformation of A. foetidus and selection of transformed TK- A. foetidus have been studied. By using this system, several transformed A. foetidus which contained HBsAg gene derived bf a promoter H8 cloned from genomic DNA of A. foetidus were isolated. It was demonstrated that HBsAg gene was integrated into the chromosome DNA of A. foetidus by Southern blot after many passages of spores. ELISA showed that HBsAg was positive in the growth medium (p/n = 20). The 22 nm particles which were very similar to the HBsAg particles in human serum were found in the growth medium by immunoelectromicroscope. Western blot also gave the specific bands. All these data showed that HBsAg gene was expressed in A. foetidus and the products were secreted into the growth medium. The selection system using TK gene as marker could generally be used to study the expression of foreign gene in A. foetidus.     

九年制义务教育全日制初级中学生物学教学大纲（初审稿）

本刊本期刊登了九年制义务教育《全日制初级中学生物学教学大纲(初审稿)》供广大教师学习。这一大纲1990年已在一些地区和省市的部分地区试用,待取得经验后将在全国逐步推行。根据这一大纲现已有8个地区和单位编写了或正在编写相应的教材,有的已正在进行试点。采取一纲多材的办法是为了贯彻因地制宜和百花齐放、百家争鸣的方针促进教材的改革。本刊将有重点地、有选择地介绍有关教材,希读者注意阅读。     

《自然一细胞生物学》：科学家发现肿瘤发生新机制

中国科学技术大学生命科学院吴缅教授与美国宾夕法尼亚大学医学院杨小鲁教授合作，在癌症代谢机制研究中取得一项新的突破性发现。即证实p73蛋白激活了癌细胞中的磷酸戊糖途径，支持了肿瘤细胞的增殖。     

浅析数字监控系统

近些年,人们对数字监控技术的需求量越来越大,被广泛的应用于生活与生产的各个领域。数字监控系统主要由摄像头、监控现场主机与远程端主机三部分组成,与模拟系统相比,它具有系统配置简单,录像方式多样,检索方式灵活,可以方便的进行警报系统的设置,访问也会有清楚的记录。本文深入分析了数字监控系统的特点以及关键技术。     

基于显微图像的牛蛙肠系膜微循环观察

在动物活体上进行微循环观察是微循环研究的主要手段,肠系膜是脏器微循环的良好观察部位。通过医学图像分析系统可直接观察、录像、测量并记录正常状态下牛蛙肠系膜微血管的口径及开放数量、血流流态与流速、血流量等指标,并可观察滴加药物后上述指标发生的变化,探讨微循环的功能状态及药物等因素对微循环的影响。