辽宁省菜田生态系统组分优势值与系统优势度分析

辽宁省菜田生态系统组分优势值与系统优势度分析周宝利，葛晓光李宁义（沈阳农业大学园艺系，１１０１６１）（沈阳市城建中专，１１００１３）ＡｎａｌｙｓｉｓｏｎＳｕｐｅｒｉｏｒｉｔｙＶａｌｕｅａｎｄＤｏｍｉｎａｎｃｅＤｅｇｒｅｅｏｆＶａｒｉｏｕｓＣｏｍｐｏｎ...     

建立以TK基因为标记将外源基因导入臭曲霉（Asper—gillus...

A new system for selection of transformed Aspergillus foetidus was reported. In this system, TK- A. foetidus which were constructed by homologous recombination of mutated TK gene of vaccinia virus with TK gene of A. foetidus were screened by adding BUdR in agar plates. Conditions for screen of TK+ A. foetidus strain, transformation of A. foetidus and selection of transformed TK- A. foetidus have been studied. By using this system, several transformed A. foetidus which contained HBsAg gene derived bf a promoter H8 cloned from genomic DNA of A. foetidus were isolated. It was demonstrated that HBsAg gene was integrated into the chromosome DNA of A. foetidus by Southern blot after many passages of spores. ELISA showed that HBsAg was positive in the growth medium (p/n = 20). The 22 nm particles which were very similar to the HBsAg particles in human serum were found in the growth medium by immunoelectromicroscope. Western blot also gave the specific bands. All these data showed that HBsAg gene was expressed in A. foetidus and the products were secreted into the growth medium. The selection system using TK gene as marker could generally be used to study the expression of foreign gene in A. foetidus.     

浅析数字监控系统

近些年,人们对数字监控技术的需求量越来越大,被广泛的应用于生活与生产的各个领域。数字监控系统主要由摄像头、监控现场主机与远程端主机三部分组成,与模拟系统相比,它具有系统配置简单,录像方式多样,检索方式灵活,可以方便的进行警报系统的设置,访问也会有清楚的记录。本文深入分析了数字监控系统的特点以及关键技术。     

基于显微图像的牛蛙肠系膜微循环观察

在动物活体上进行微循环观察是微循环研究的主要手段,肠系膜是脏器微循环的良好观察部位。通过医学图像分析系统可直接观察、录像、测量并记录正常状态下牛蛙肠系膜微血管的口径及开放数量、血流流态与流速、血流量等指标,并可观察滴加药物后上述指标发生的变化,探讨微循环的功能状态及药物等因素对微循环的影响。     

浅谈树的移植

本文根据各种树木的特性,提出了树在移植过程中的注意事项,包装运输及过程。     

具有重要应用价值的真核表达系统

基于Ｔ７ＲＮＡ聚合酶与Ｔ７强启动子（Φ１０）间的特异认识而建立起来的偶联表达系统在大肠杆菌中已取得了令人满意的结果。最后，该系统在酵母、昆虫、浦乳动物细胞和植物细胞中陆续实现了高效表达。Ｔ７ＮＲＡ聚合酶－Φ１０启动子偶联表达系统的相对独立性和高效性，不仅预示着该系统可以在真核基因工程中发挥重要作用，而且其独特的作用机制为人们探索新的特异高效真核表达系统提供了一个可供借鉴的模式。