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通过对比试验,筛选出含3.0%牛血清白蛋白的BWW培养基和2·0mol/L最终浓度的二甲基 亚砜(DMSO)冷冻保护剂配制成冷冻液,将金黄仓鼠卵以0.3-1·0℃/min的冷冻速率分别降温至 -40℃和-80℃,再浸入液氮中冷冻保存(3~31天)。结果表明:冷冻降温速率以0·3℃-0·5℃/min 为宜,降温到一80℃浸入液氮的冻卵存活率(63%)比-40℃(46%)的高。将解冻后存活卵去除 透明带,与人获能精子进行体外穿透试验,穿卵率达34. 4%,与新鲜卵对照组(50%)相比,无统 计学差异(P>0·05);扫描电镜观察结果与压片光镜观察相吻合。 相似文献
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The mitochondrion is the major energy provider to power sperm motility. In mammals, aside from the nuclear genome, mitochondrial DNA (mtDNA) also contributes to oxidative phosphorylation to impact production of ATP by coding 13 polypeptides. However, the role of sperm mitochondria in fertilization and its final fate after fertilization are still controversial. The viewpoints that sperm bearing more mtDNA will have a better fertilizing capability and that sperm mtDNA is actively eliminated during early embryogenesis are widely accepted. However, this may be not true for several mammalian species, including mice and humans. Here, we review the sperm mitochondria and their mtDNA in sperm functions, and the mechanisms of maternal mitochondrial inheritance in mammals. 相似文献
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Recent studies have revealed that sexually selected traits may signal sperm quality and hence male fertilisation ability. There is also evidence that the expression of male sexual ornamentation and associated sperm characteristics depend on an indi- vidual's ability to cope with oxidative stress. Carotenoids are known for their antioxidant properties and carotenoid-based orna- ments might represent honest signals as these pigments can be traded offbetween the investment in sexual ornamentation, sperm function as well as immune response. In this study, we examined the relationship between sexual ornamentation (breeding colo- ration) and sperm characteristics (e.g., velocity and morphology) in the three-spined stickleback Gasterosteus aeuleatus, an ex- ternally fertilising fish species, in which sperm competition commonly occurs. During the breeding season males are sperm limi- ted and develop a conspicuous carotenoid-based coloration, which is under strong pre-copulatory sexual selection due to female mate choice and male-male competition. The results of the present study show that the expression of stickleback male breeding coloration is significantly positively associated with the linearity of sperm movement, whereas sperm morphology (head length to tail length ratio) is significantly negatively related to the trajectory of sperm movement. Moreover, there is some support for the phenotype-linked fertility hypothesis as the intensity of male red breeding coloration is significantly positively correlated with sperm velocity, which is supposed to be an important determinant of fertilisation success in external fertilisers, indicating the honesty of the sexually selected nuptial red coloration. 相似文献
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受精是一个复杂的生物学过程,精子必须具备良好的运动功能,同时需经获能和顶体反应才能实现受精。通过添加外源性物质,来改善精子体外存活时间,提高精子的运动能力,尤其是冷冻保存精子解冻后的受精能力,被认为是借助实验手段在体外改善精子功能,从而提高受精率的一个有效途径。可添加的外源性生物活性物质种类很多,通常有咖啡因、氨基多糖类物质、己酮可可碱、生殖激素类物质、细胞因子、维生素、抗氧化剂、牛黄酸和一些酶类物质等。本文对精子常用的外源性添加物质的研究进展进行了总结,在此基础提出了寻找新的外源添加物质的思路和途径。 相似文献
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