CD4+CD25+调节性T细胞与人类获得性免疫缺陷病毒感染

CD4 CD25 是调节性T细胞中功能最重要的一类.它是一类具有特殊免疫调节功能的T细胞亚群.它能够抑制自身免疫病的发生和发展,参与肿瘤免疫的调节,同时在感染和移植免疫中也发挥着极其重要的作用.T细胞的这一亚群具有免疫调节和免疫抑制的特性,新近发现它亦与爱滋病的发生、发展关系密切.HIV进入人体后,CD4 CD25 调节性T细胞抑制了机体的免疫效应但它也同时被感染,最终由于细胞毒的作用而死亡.由于调节性T细胞数量的减少不能有效的发挥其抑制作用,HIV持续的过度活化使得T细胞逐渐耗竭说明在HIV发生、发展的不同阶段Treg细胞可能都发挥了免疫抑制作用,但是却对HIV感染与爱滋病发病的进程产生了不同的效应.此外,CD4 CD25 调节性T细胞还与HIV病毒的持续存在密切相关.本文就CD4 CD25 调节性T细胞与人类获得性免疫缺陷病毒(HIV)感染之间关系进行初步的探讨.     

宫颈癌患者外周血CD4+CD25+high调节性T细胞的表达及意义

目的:探讨宫颈癌患者外周血中CD4~ CD25~( high)调节性T(regulator T cells,Tr)的表达及意义。方法:采用流式细胞术检测52例宫颈癌患者,35例健康女性外周血中CD4~ CD25~( high)Tr、细胞毒性T细胞(cytotoxic T lymphocytes,CTL)和NK细胞,采用ELISA检测血清中-干扰素(interferon,IFN-)的表达水平。结果:宫颈癌患者外周血CD4~ CD25~( high)Tr占CD4~ T淋巴细胞的百分比为(7.18±2.32)%,高于健康女性组(P<0.05);宫颈癌患者外周血CD4~ CD25~( high)Tr水平与CTL、NK细胞及IFN-水平呈负相关。结论:宫颈癌患者外周血中具免疫抑制活性的CD4~ CD25~( high)Tr水平较高,参与宫颈癌患者的肿瘤免疫抑制。     

The effect of external Ca^2＋ and Ca^2＋—channel modulators on red—light—induced swelling of protoplasts of Phaseolus radiatus L.

Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean(Phaseolus radiatusL.)was observed only when Ca^2 ions were present in the medium.The optimal CaCl2 concentration was 250μM,Swlling response declined when Ca^2 was supplied into the medium after red light irradiation.The Ca^2 -chelator EGTA eliminated the red-light-induced swelling and 45Ca^2 accumulation in the protoplasts.In conltrast,A23187,a Ca^2 -ionophore,could mimic the effect of red light in darkness.These results indicate that Ca^2 may play a role in light signal transduction.In addition,swelling response was prevented by TFP and CPZ(both are CaM antagonists),implying the involvement of CaM in red-light-induced and Ca^2 -dependent protoplast swelling.     

Determination of oxytetracycline hydrochloride in milk and egg white samples using Ru(bipy)32+–Ce(SO4)2 chemiluminescence

A novel, rapid and sensitive chemiluminescence (CL) method for the determination of oxytetracycline hydrochloride (OTCH) is described in this paper. The presented method was based on the fact that OTCH could immensely enhance the CL of the reaction of cerium sulfate and tris(2,2‐bipyridyl) ruthenium (II) in acidic medium. Under optimal experimental conditions, CL intensity was favorably linear for OTCH in the range 5.0 × 10^?7 to 5.0 × 10^?5 g/ml, with a detection limit of 1.5 × 10^?7 g/ml (S/N = 3). The relative standard detection was 4.76% for 5.0 × 10^?6 g/ml (n = 11). This method was successfully applied to the analysis of OTCH in milk and egg white samples. According to the results of the kinetic curves for OTCH in the Ru(bipy)₃²⁺–Ce(SO₄)₂ CL system, together with CL and ultraviolet (UV)–visible spectra, the possible mechanism of the CL reaction is discussed briefly.     

Effects of Ce doping on the luminescent property of Ca3SiO4Cl2:Eu phosphor for green lighting

White light‐emitting diodes (LEDs) for green lighting are new solutions for energy saving and environmental protection. Ca₃SiO₄Cl₂:Ce,Eu is an efficient phosphor for white LEDs. Effective energy transfer from Ce³⁺ to Eu²⁺ occurs in Ca₃SiO₄Cl₂:Ce,Eu due to good spectrum overlap between the emission band of Ca₃SiO₄Cl₂:Ce and the excitation band of Ca₃SiO₄Cl₂:Eu, and hues vary systematically from blue to green at different Ce concentrations. A great improvement in the luminescent property of Ca₃SiO₄Cl₂:Eu has been observed on Ce³⁺ doping, which is attributed to energy transfer from Ce³⁺ to Eu²⁺ and an increase in the number of luminescent centers (Eu²⁺) on Ce doping. The optimal sample has a quantum efficiency of up to 75%, and can be an efficient green phosphor for white LEDs. Copyright © 2014 John Wiley & Sons, Ltd.     

60Co-γ射线诱变连麦2号M3代主要农艺性状的变异及多元分析

用60Co-γ射线诱变小麦连麦2号,对M3代292个穗行进行了主要农艺性状的变异及多元分析.结果表明:各性状的变异幅度是不同的,变异系数从大到小依次为:穗数＞千粒重＞穗长＞小穗数＞株高＞行粒重＞退化小穗数＞穗粒数＞穗粒重;变异率从大到小依次为:穗数＞千粒重＞株高=行粒重＞穗粒数＞穗长＞穗粒重＞小穗数＞退化小穗数.穗数、...     

60Co-γ射线诱变选育富硒酵母菌株

本试验以产朊假丝酵母菌(Candida utilis)为出发菌株,通过60Co-γ射线诱变,采用Na2SeO3抗性平板初筛和摇瓶培养复筛的方法,获得了一株高硒和高硒蛋氨酸含量的菌株CU7,与出发菌株相比,生物量达到10.58g/L、总硒提高了2.01倍.硒蛋氨酸的含量提高了2.44倍,硒蛋氨酸与总硒的比值也由49.85...     

MicroRNA-141 在上皮性卵巢癌患者组织和血清中的表达 及临床意义的探讨

目的:探讨微小RNA-141(miR-141)在卵巢癌患者组织和血清中的表达情况并初步探讨其作为肿瘤标记物早期诊断上皮性卵巢癌的可行性。方法:采用实时荧光定量逆转录聚合酶链反应(real-time RT-PCR)检测16例上皮性卵巢癌患者和4例正常人卵巢组织及血清标本中miR-141的表达;检测4例良性卵巢肿瘤血清中miR-141的表达。结果:miR-141在卵巢癌患者组织中相对表达量分别为(72.846±76.671)显著高于正常人(2.869±3.201)(P<0.05);miR-141在卵巢癌患者血清中相对表达量(31.581±52.885)显著高于良性卵巢肿瘤患者(0.668±1.196)和正常人(1.690±1.697)(P<0.05),后两组间表达无差异(P>0.05);miR-141表达随卵巢癌临床分期的进展呈上升趋势(P<0.01),在无淋巴结转移组明显高于有淋巴结转移组(P<0.05),与组织分级和CA125的升高无关(P>0.05)。在组织中miR-141表达水平与上皮性卵巢癌临床病理特征均未见明显差异(P>0.05)。结论:miR-141可能在上皮性卵巢癌的发生发展中发挥癌基因的作用;miR-141用于检测上皮性卵巢癌敏感性和特异度较高,有望成为上皮性卵巢癌早期诊断的新指标。     

新疆艾比湖湿地自然保护区植物的生态分组

应用列联表的χ~2检验和Spearman相关系数研究了艾比湖湿地自然保护区植物的种间联结性和相关性.依据测定结果,将艾比湖湿地自然保护区的植物划分为4个生态种组:梭梭+盐节木+白麻+盐爪爪+琵琶柴+碱蓬+花花柴+骆驼刺+绢蒿+白刺+芦苇、小獐茅+多枝柽柳+滨藜+小叶碱蓬+盐豆木+鹅绒藤+盐穗木、胡杨+白梭梭、奶浆草+甘草+西北天门冬+赖草+罗布麻.各生态种组内,物种间具备最大的种问联结性和相关性.长期的演替过程使各生态种组对生境有最大的适应能力,各自分别生活于过渡带的盐化平原低地、湖滨盐沼地、荒漠和荒漠优势种间的阳斑.今后在艾比湖湿地自然保护区植物的保护和恢复中,以整个生态种组的角度出发较单个植物的保护和恢复更为有效.