硒对NO诱导的内皮细胞内游离钙离子浓度变化的影响

用Fura-2显微荧光测钙技术,研究了用外源性一氧化氮（NO）供体S-亚硝基谷胱甘肽（GSNO）诱导的,人脐静脉内皮细胞系ECV-304细胞胞内游离钙离子浓度（［Ca²⁺］_i ）升高以及硒的抑制效应.结果表明,GSNO作用于ECV-304细胞,短时间内即可导致其胞内游离钙离子浓度升高.胞外液换为无钙液或向胞外液中加入CdCl₂（1 mmol/L）对GSNO引起的［Ca²⁺］_i升高无影响.提示,GSNO刺激主要引起胞内钙库释放.而且,一氧化氮清除剂血红蛋白（Hb）对这一过程有抑制作用,说明GSNO引起的胞内钙库释放由NO介导.经亚硒酸钠（1 μmol/L）处理的细胞,其NO引起的［Ca²⁺］_i升高幅度明显被抑制,说明NO的这种作用可能与细胞的氧化还原状态有关.     

Systematics of the Monodelphic Species of Trichodoridae (Nematoda: Diphtherophorina) With Descriptions of a New Genus and Four New Species

To show the relationship of the monodelphic species of Trichodoridae with the nominal taxa, the genus Monotrichodorus is redefined, with the addition of one new species. The new genus Allotrichodorus is proposed on the basis of new species found in Brazil; and one new species is described in the genus Paratrichodorus.     

麋鹿繁殖行为和粪样激素水平变化的关系

１９９７年７－９月和１９９８年３－７月间,在江苏大麋鹿保护区共观察到麋鹿的２７种繁殖行为,雄鹿有１７种,雌鹿有１０种,在１９９８年３－７月间,利用放射免疫分析法（ＲＩＡ）测定了鹿麋样中的性激素水平,包括雄鹿的睾酮,雌鹿的酮,雌鹿的孕酮、雌二醇和雌三醇。实验结果表明：１许多繁殖行为的发生频次和持续时间表现出明显的季节变化（Ｐ〈０．０５）,如雄鹿的求偶行为、标记行为和性行为及雌鹿的性行为等在发情期有一     

Queuosine deficient tRNAHis and tRNAAsp from the spleens of young mice,erythroleukemic tumoral spleens and cultured Friend cells

tRNAs were isolated from the spleens of young mice, erythroleukemic spleens and cultured Friend cells. Queuosine (Q) deficient tRNAs were labelled in their anticodon with radioactive guanine using the exchange reaction catalyzed by E. coli tRNA-guanine transglycosylase. tRNAs were then specifically aminoacylated. In both normal and tumoral spleen (TF-P10), tRNA^His was the main guanine containing (G) isoacceptor as shown by RPC-5 chromatography. In vitro, the tumor derived cell line (TF-P10c) retained the G-tRNA^His species while Friend cell line, clone 707 (FLC), did not. A common feature found in both cultured cell lines was the high level of G-tRNA^Asp. The meaning of the relative abundance of Q-deficient tRNA^His and of tRNA^Asp observed in transformed cells of erythroid origin is discussed.     

New fossils of ascomycetous anamorphic fungi from Baltic amber

Three new fossils of saprotrophic fungi are presented and described from Baltic amber, dated to Eocene epoch (Paleogene, upper to mid-Eocene). All belong to Ascomycota and are represented by hyphae as well as asexual reproduction structures allowing to assign them to present genera, respectively Periconia, Penicillium and Scopulariopsis. These material provide both the first and the oldest known fossil record of the mentioned taxa, making these data valuable for the knowledge about the evolutionary history of the Ascomycota.     

Changes of enzyme activity in lipid signaling pathways related to substrate reordering

The static fluid mosaic model of biological membranes has been progressively complemented by a dynamic membrane model that includes phospholipid reordering in domains that are proposed to extend from nanometers to microns. Kinetic models for lipolytic enzymes have only been developed for homogeneous lipid phases. In this work, we develop a generalization of the well-known surface dilution kinetic theory to cases where, in a same lipid phase, both domain and nondomain phases coexist. Our model also allows understanding the changes in enzymatic activity due to a decrease of free substrate concentration when domains are induced by peptides. This lipid reordering and domain dynamics can affect the activity of lipolytic enzymes, and can provide a simple explanation for how basic peptides, with a strong direct interaction with acidic phospholipids (such as beta-amyloid peptide), may cause a complex modulation of the activities of many important enzymes in lipid signaling pathways.     

Botulinum toxin's axonal transport from periphery to the spinal cord

Axonal transport of enzymatically active botulinum toxin A (BTX-A) from periphery to the CNS has been described in facial and trigeminal nerve, leading to cleavage of synaptosomal-associated protein 25 (SNAP-25) in central nuclei. Aim of present study was to examine the existence of axonal transport of peripherally applied BTX-A to spinal cord via sciatic nerve. We employed BTX-A-cleaved SNAP-25 immunohistochemistry of lumbar spinal cord after intramuscular and subcutaneous hind limb injections, and intraneural BTX-A sciatic nerve injections. Truncated SNAP-25 in ipsilateral spinal cord ventral horns and dorsal horns appeared after single peripheral BTX-A administrations, even at low intramuscular dose applied (5 U/kg). Cleaved SNAP-25 appearance in the spinal cord after BTX-A injection into the sciatic nerve was prevented by proximal intrasciatic injection of colchicine (5 mM, 2 μl). Cleaved SNAP-25 in ventral horn, using choline-acetyltransferase (ChAT) double labeling, was localized within cholinergic neurons. These results extend the recent findings on BTX-A retrograde axonal transport in facial and trigeminal nerve. Appearance of truncated SNAP-25 in spinal cord following low-dose peripheral BTX-A suggest that the axonal transport of BTX-A occurs commonly following peripheral application.