Aerobic Respiration of Kappa Particles from Paramecium aurelia,Stock 51*

SYNOPSIS. Kappa particles from killer cultures of stock 51 Paramecium aurelia were purified and their respiration measured polarographically. The slight bacterial contaminations in the kappa preparations were not significant. Freshly collected kappa in dilute buffer at room temperature had an endogenous Q_O2 of 17.0 ± 1.6 μl/mg dry weight/hr (mean ± standard error). The Q_O2 decayed 50% in 5 hr. Among the sugars tested only glucose and sucrose increased the respiratory rate of kappa. The di- and tri-carboxylates of the tricarboxylic acid cycle stimulated the respiration of kappa. KCN, CO and 2-heptyl-4-hydroxyquinoline N-oxide (HOQNO) inhibited respiration. These findings ensure an organismic status for kappa and justify the belief that it is bacterial in origin.     

中国石杉属(狭义)小杉兰组的分类学研究

本文将石杉科石杉属(狭义)分为两组,即小杉兰组Sect．Huperzia和蛇足石杉组Sect．Serratae (Rothm．)Holub,对小杉兰组的概念进行了修订并对国产有关种类进行了分类学研究。共记载国产小杉 兰组植物12种1变种,并包括1个新组合:Huperzia quasipolytrichoides(Hayata)Ching var. rectifolia (J．F．Cheng)H．S．Kung et L．B．Zhang,2个新异名:H．hupehensis Ching和H．whangshanensisChing et P．C．Chiu．     

中国及越南东京毛跳(虫斧)记述

毛跳(虫斧)属(Hespera)隶鞘翅目,(虫斧)科,跳(虫斧)亚科。它和寡毛跳(虫斧)属(Lupero-morpha)近缘,作者在另一报告内曾述及这两属的密切系统关系;由於这种关系,我们在监定寡毛跳(虫斧)类时,亦研究了本属内的中国和越南东京的种类,本文即为这项研究结果的报告。     

白眼家蝇的特性及其在生物测定上的应用

1961年8月,在北京郊区农药厂附近采集了一些家蝇,经过室内培养后,发现有7个白眼家蝇,随即进行分离培养,成为白眼变种品系。 白眼家蝇变种在国内还是第一次发现,国外亦只是近二三年来才有报道(Sullivan及Hiroyoshi,1960;Tsukamoto,1961),他们发现的变种可能属于 Musca domestica domes-tica L.而我们发现的是属于 Musca domestica vicina M.。     

Induction of Antibiotic Resistance in Paramecium tetraurelia by the Bacterial Gene APH-3'-II

We have generated a transformation marker for Paramecium using a Paramecium expression vector (pPXV) and the open reading frame (ORF) of the bacterial antibiotic resistance gene aminoglycoside 3'-phosphotransferase-II (APH-3'-II or neo^r) from the transposon Tn5. The expression vector contained a small multiple cloning site between the 5' and 3' non-coding regions of the calmodulin gene, and Tetrahymena telomere sequences for the stability of the plasmid in Paramecium. After the neo^r ORF was inserted, the plasmid was referred to as pPXV-NEO. Delivery of approximately 10–20 picoliters of linearized PXV-NEO at > 2000 copies/pl into the macronucleus effected 100% transformation. Southern and Northern blot hybridization showed the presence of neo^r-specific DNA and RNA, respectively, in all of the transformed clones but not in the untransformed clones. The degree of resistance to G-418, and the concentrations of neo^r-specific DNA and neo^r-specific RNA in the clones were proportional to the concentration of the vector injected. We have demonstrated that when the linearized plasmid was injected into the macronucleus, the prokaryotic sequence conferred an antibiotic resistance to Paramecium despite codon-usage differences.     

六烷醣酸锑(三价)钾及纳封於小白鼠髓内日本血吸虫的作用(日本血吸虫实验治疗研究之三)

本文报告用六烷醣酸娣(三价)钾及钠治疗人工感染的小白鼠,结果以20克體重每日给药0.6毫克,连续注射15针的效果最佳,此剂量巳接近痊愈剂量。     

氰酸气熏蒸防治黑点介壳虫 Parlatoria zizyphus (Lucas)的初步试验

黑点介壳虫Parlatoria zizyphus(Lucas)系福建省柑桔类介壳虫中发生较严重者。目前以及今後对於这种介壳虫及其他介壳虫的防治,无论在柑桔外销或增产上,都有极重大的意义。氰酸气天幕熏蒸是防治介壳虫重要方法之一,植物检验部门曾要     

棉蚜对“1059”抗药性的测定

一、前言 近十年来,各种农业害虫对杀虫剂的抗药性,已陆续被发现。其中关于蚜虫的抗药性,虽然亦有记述,但增加抗药性的倍数不多,并不发展得很严重。例如,Michelbacher(1954)曾报导,核桃蚜(Chramaphis juglandicola Kltb.)对1605的抗性为7倍;Knipling(1954)报导,红薯蚜(Macrosiphum solanifolii Ashm.)对 DDT及1605有抗性; Anthron(1955)报导,桃绿蚜(Myzus persicae Sulzer)对1605有抗性,但用1059防治,效果仍很好;Stern(1958)报导,苜蓿斑蚜(Therioaphis maculata Buckton)对1605的抗性约4倍。     

中国守瓜属记述

守瓜又名瓜萤,是瓜类作物的重要害虫。在分类系统上,守瓜隶属于叶甲科、守瓜亚科(Galerucinae)、守瓜属(Aulacophora)。本篇报告的目的,即为记述我国及越南、东京的本属种类特征和分布情况,以备有关工作者进行防治研究的参考。计共记述17种,其中国产种15,仅见于越南者2种,有3种是新种,另4种系我国初次记载。 我国古代人民在和害虫的斗争中,对守瓜的习性即已有相当认识,尔雅释虫篇提到“?舆父守瓜”一节,有这样的说明:“?舆父一名守瓜,黄甲小虫喜食瓜叶,因名守瓜”。这