水稻耐贮藏基因（lox-3）DH作图群体构建方法的比较研究

以与水稻耐贮藏材料（Daw Dam）有丰富分子标记多态性的常规材料2070、明恢63、Dulur/协青早B为亲本,分别与之杂交,采用改良一步成苗花药培养法对其F1代进行花药培养,以一步成苗法为对照,评价新方法在构建耐贮藏基因DH作图群体中的应用潜力。研究结果表明,改良一步成苗法显著地提高了愈伤组织分化率、植株再生率、绿苗率、绿苗素质,而且得到的加倍单倍体系田间表现良好,是构建耐贮藏基因DH作图群体的理想方法,但在不同基因型间表现有一定的差异性,其中Daw Dam/2070、Daw Dam/明恢63组合的培养效率有较大增幅。利用改良一步成苗法已获得一定数量的DH群体,为开展进一步研究奠定了基础。 Abstract:Crosses were produced by using a storable rice variety (Daw Dam) and 2070,Ming Hui 63,as well as Dulur/Xie Qing Zao B as parents,among these materials there are abundant polymorphisms of DNA molecular markers.Their F1's were applied to generate DH lines by an improved method of direct induction of pollen plant.The method of the direct induction of pollen plant was used as check treatment to evaluate the improved method.The results showed that the improved method increased the re-differentiation percentage of pollen callus,the percentage of regeneration plantlets,and the percentage of green regeneration plantlets and produced more vigorous seedlings.The improved method was a competent method of establishing DH population for mapping lox-3 gene.It was also showed that the efficiency of the new method was different among genotypes and out of three experimental crosses Daw Dam/2070 and Daw Dam/Minghui 63 showed more efficient.More than 60 DH lines have been obtained in this experiment.     

籼稻标记性状等基因系的构建

选用涉及水稻(Oryza sativa L.)全部12条染色体的、表现简单遗传且易于识别的形态标记材料27份,以早籼品种浙辐802为轮回亲本,经10余次回交,转育成一套籼型标记等基因系.在此基础上,对同一染色体上的标记进行聚合,育成了15份双标记等基因系.该套材料除所带标记性状外,生育期、株高、分蘖力和穗子大小等主要农艺性性状与轮回亲本基本相仿.     

水稻株高、抽穗期和有效穗数的QTL与环境的互作分析

株高、抽穗期和有效穗数是水稻的重要农艺性状,合适的株高、抽穗期和有效穗数对水稻的高产稳产是至关重要的。该实验应用中156/谷梅2号的重组自交系(RIL)群体,建立由168个DNA分子标记组成的遗传连锁图,以一年两季作为不同的环境效应,对水稻株高、抽穗期和有效穗数进行了非条件和条件QTL定位,在非条件QTL定位中共检测到7个株高QTLs、5个抽穗期QTLs和3个有效穗数QTLs和10对加加上位性互作位点,条件QTL定位结果表明,抽穗期这一性状对株高和有效穗数QTLs的表达既有抑制作用,也有较大的贡献率。     

籼稻标记性状等基因系的构建

选用涉及水稻(Oryza sativa L．)全部12条染色体的、表现简单遗传且易于识别的形态标记材料27份,以早籼品种浙辐802为轮回亲本,经10余次回交,转育成一套籼型标记等基因系。在此基础上,对同一染色体上的标记进行聚合,育成了15份双标记等基因系。该套材料除所带标记性状外,生育期、株高、分蘖力和穗子大小等主要农艺性状与轮回亲本基本相仿。