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Stable transformation of the cotton plastid genome and maternal inheritance of transgenes 总被引:23,自引:0,他引:23
Chloroplast genetic engineering overcomes concerns of gene containment, low levels of transgene expression, gene silencing, positional and pleiotropic effects or presence of vector sequences in transformed genomes. Several therapeutic proteins and agronomic traits have been highly expressed via the tobacco chloroplast genome but extending this concept to important crops has been a major challenge; lack of 100 homologous species-specific chloroplast transformation vectors containing suitable selectable markers, ability to regulate transgene expression in developing plastids and inadequate tissue culture systems via somatic embryogenesis are major challenges. We employed a Double Gene/Single Selection (DGSS) plastid transformation vector that harbors two selectable marker genes (aphA-6 and nptII) to detoxify the same antibiotic by two enzymes, irrespective of the type of tissues or plastids; by combining this with an efficient regeneration system via somatic embryogenesis, cotton plastid transformation was achieved for the first time. The DGSS transformation vector is at least 8-fold (1 event/2.4 bombarded plates) more efficient than Single Gene/Single Selection (SGSS) vector (aphA-6; 1 event per 20 bombarded plates). Chloroplast transgenic lines were fertile, flowered and set seeds similar to untransformed plants. Transgenes stably integrated into the cotton chloroplast genome were maternally inherited and were not transmitted via pollen when out-crossed with untransformed female plants. Cotton is one of the most important genetically modified crops ($ 120 billion US annual economy). Successful transformation of the chloroplast genome should address concerns about transgene escape, insects developing resistance, inadequate insect control and promote public acceptance of genetically modified cotton. 相似文献
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Generation of fertile transplastomic soybean 总被引:26,自引:0,他引:26
Dufourmantel N Pelissier B Garçon F Peltier G Ferullo JM Tissot G 《Plant molecular biology》2004,55(4):479-489
We describe here the development of a plastid transformation method for soybean, a leguminous plant of major agronomic interest. Chloroplasts from embryogenic tissue of Glycine max have been successfully transformed by bombardment. The transforming DNA carries a spectinomycin resistance gene (aadA) under the control of tobacco plastid regulatory expression elements, flanked by two adjacent soybean plastome sequences allowing its targeted insertion between the trnV gene and the rps12/7 operon. All generated spectinomycin resistant plants were transplastomic and no remaining wild type plastome copies were detected. No spontaneous mutants were obtained. The transformation efficiency is similar to that of tobacco plastids. All transplastomic T0 plants were fertile and T1 progeny was uniformly spectinomycin resistant, showing the stability of the plastid transgene. This is the first report on the generation of fertile transplastomic soybean. 相似文献
5.
Phytoremediation: an overview of metallic ion decontamination from soil 总被引:23,自引:0,他引:23
Singh OV Labana S Pandey G Budhiraja R Jain RK 《Applied microbiology and biotechnology》2003,61(5-6):405-412
In recent years, phytoremediation has emerged as a promising ecoremediation technology, particularly for soil and water cleanup of large volumes of contaminated sites. The exploitation of plants to remediate soils contaminated with trace elements could provide a cheap and sustainable technology for bioremediation. Many modern tools and analytical devices have provided insight into the selection and optimization of the remediation process by plant species. This review describes certain factors for the phytoremediation of metal ion decontamination and various aspects of plant metabolism during metallic decontamination. Metal-hyperaccumulating plants, desirable for heavily polluted environments, can be developed by the introduction of novel traits into high biomass plants in a transgenic approach, which is a promising strategy for the development of effective phytoremediation technology. The genetic manipulation of a phytoremediator plant needs a number of optimization processes, including mobilization of trace elements/metal ions, their uptake into the root, stem and other viable parts of the plant and their detoxification and allocation within the plant. This upcoming science is expanding as technology continues to offer new, low-cost remediation options. 相似文献
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杂交粳稻超高产群体的冠层结构特点研究 总被引:9,自引:0,他引:9
研究了杂交粳稻超高产群体的苗穗粒结构及干物重在冠层不同层次、不同器官的分布,叶面积配置及光强分布等冠层结构特点.结果表明,杂交粳稻超高产群体冠层总干物重及40cm以下、40~60cm、60~80cm和80cm以上4个层次的干物重分别比常规粳稻高32.29%及29.12%、13.95%、16.45%和100.17%.杂交粳稻叶片(同化器官)与穗(库器官)干重分别占总干物重的248%与12.8%.高于常规稻;而叶鞘与茎(贮藏器官)的干重分别占总干物重的33.6%和28.9%,低于常规粳稻.杂交粳稻超高产群体冠层叶面积配置比较合理,齐穗期40cm以上冠层LAI达5.44.冠层光强上下分布比较均匀,60cm以下冠层光强比常规稻高13.1%~37.0%,而60cm以上冠层光强比常规稻低5.9%~12.2%;20cm、20~40cm、40~60cm和60~80cm各层消光系数分别比常规粳稻低35.1%、13.5%、29.1%和17.2%. 相似文献
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A Contrast of the Plasma Membrane Lipid Composition of Oat and Rye Leaves in Relation to Freezing Tolerance 总被引:25,自引:2,他引:23 下载免费PDF全文
The lipid composition of the plasma membrane isolated from leaves of spring oat (Avena sativa L. cv Ogle) was vastly different from that of winter rye (Secale cereale L. cv Puma). The plasma membrane of spring oat contained large proportions of phospholipids (28.8 mol% of the total lipids), cerebrosides (27.2 mol%), and acylated sterylglucosides (27.3 mol%) with lesser proportions of free sterols (8.4 mol%) and sterylglucosides (5.6 mol%). In contrast, the plasma membrane of winter rye contained a greater proportion of phospholipids (36.6 mol%), and there was a lower proportion of cerebrosides (16.4 mol%); free sterols (38.1 mol%) were the predominant sterols, with lesser proportions of sterylglucosides (5.6 mol%) and acylated sterylglucosides (2.9 mol%). Although the relative proportions of individual phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine, and the molecular species of these two phospholipids were similar in oat and rye, the relative proportions of di-unsaturated species of these two phospholipids were substantially lower in oat than in rye. The relative proportions of sterol species in oat were different from those in rye; the molecular species of cerebrosides were similar in oat and rye, with only slight differences in the proportions of the individual species. After 4 weeks of cold acclimation, the proportion of phospholipids increased significantly in both oat (from 28.8 to 36.8 mol%) and rye (from 36.6 to 43.3 mol%) as a result of increases in the proportions of phosphatidylcholine and phosphatidylethanolamine. For both oat and rye, the relative proportions of di-unsaturated species increased after cold acclimation, but the increase was greater in rye than in oat. In both oat and rye, this increase occurred largely during the first week of cold acclimation. During the 4 weeks of cold acclimation, there was a progressive decrease in the proportion of cerebrosides in the plasma membrane of rye (from 16.4 to 10.5 mol%), but there was only a small decrease in oat (from 27.2 to 24.2 mol%). In both oat and rye, there were only small changes in the proportions of free sterols and sterol derivatives during cold acclimation. Consequently, the proportions of both acylated sterylglucosides and cerebrosides remained substantially higher in oat than in rye after cold acclimation. The relationship between these differences in the plasma membrane lipid composition of oat and rye and their freezing tolerance is presented. 相似文献
8.
基于1∶100万土壤数据库的中国土壤有机碳密度及储量研究 总被引:26,自引:0,他引:26
基于中国1∶100万土壤数据库,利用土壤有机碳储量和碳密度的空间化表达和计算方法研究中国土壤有机碳密度及储量.土壤空间数据库包含926个土壤类型单元,690个土属类型,94 000多个图斑;土壤属性数据库收录了7 292个全国各类型土壤的剖面数据,包括81个土壤属性字段.研究采用“土壤类型GIS连接法”实现土壤剖面有机碳密度与图形图斑连接,通过制图单元碳储量求和得出全国或者区域碳储量,并利用面积平均法计算全国及各类型土壤的有机碳平均密度.结果表明,中国的土壤面积共有928.10×104 km2,有机碳储量为89.14 Pg (1 Pg=1015 g),土壤平均碳密度9.60 kg·m-2,是目前与真值最为接近的研究结果. 相似文献
9.
Is the High Basal Level of Salicylic Acid Important for Disease Resistance in Potato? 总被引:16,自引:0,他引:16 下载免费PDF全文
Potato (Solanum tuberosum) plants contain a high basal level of salicylic acid (SA), the role of which in disease resistance is currently unclear. Here we report that, in spite of a drastic reduction in total SA levels in transgenic potato plants expressing the bacterial salicylate hydroxylase gene (nahG), there was no significant increase in disease severity when infected by Phytophthora infestans. Therefore, the high basal level of SA does not lead to constitutive resistance in healthy potato plants. However, in contrast to control plants, arachidonic acid failed to induce systematic acquired resistance (SAR) in nahG plants against P. infestans, indicating an essential role of SA in potato SAR. These results suggest that in potato the development of SAR against P. infestans may involve increased sensitivity of the plant to SA. 相似文献
10.
Many current theories of community function are based on the assumption that disturbances such as herbivory act to reduce the importance of neighbor interactions among plants. In this study, we examined the effects of herbivory (primarily by nutria, Myocastor coypus) on neighbor interactions between three dominant grasses in three coastal marsh communities, fresh, oligohaline, and mesohaline. The grasses studied were Panicum virgatum, Spartina patens, and Spartina alterniflora, which are dominant species in the fresh, oligohaline, and mesohaline marshes, respectively. Additive mixtures and monocultures of transplants were used in conjunction with exclosure fences to determine the impact of herbivory on neighbor interactions in the different marsh types. Herbivory had a strong effect on all three species and was important in all three marshes. In the absence of herbivores, the impact of neighbors was significant for two of the species (Panicum virgatum and Spartina patens) and varied considerably between environments, with competition intensifying for Panicum virgatum and decreasing for Spartina patens with increasing salinity. Indications of positive neighbor effects (mutualisms) were observed for both of these species, though in contrasting habitats and to differing degrees. In the presence of herbivores, however, competitive and positive effects were eliminated. Overall, then, it was observed that in this case, intense herbivory was able to override other biotic interactions such as competition and mutualism, which were not detectable in the presence of herbivores. 相似文献