首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   6篇
  2016年   1篇
  2012年   1篇
  2011年   1篇
  2010年   1篇
  2003年   1篇
  2002年   1篇
排序方式: 共有6条查询结果,搜索用时 46 毫秒
1
1.
花粉管细胞壁结构及胞质运动   总被引:12,自引:1,他引:11  
花粉管的极性顶端生长是将雄配子体运输到子房的过程,在高等植物有性生殖过程中起着重要的作用。花粉管的生长过程包括许多方面,其中最为重要的是花粉管细胞壁的合成和胞质运动。本文就细胞壁的结构及组成,生殖细胞和营养核的移位,细胞器以及分泌小泡的运动等方面作了较全面论述。  相似文献
2.
植物凯氏带的研究进展   总被引:4,自引:0,他引:4  
凯氏带是植物内皮层细胞径向壁和横向壁的带状增厚部分 ,近年来对其细微结构、化学成分、生理功能以及在植物体的存在部位等方面的研究工作已成为国内、外的研究热点。本文就凯氏带上述诸方面的研究进展 ,作一详尽的论述 ,同时还着重对凯氏带研究的新方法和新技术作了评述。最后对凯氏带的进一步研究工作提供新的启示  相似文献
3.
γ-聚谷氨酸生产菌的选育及培养条件研究   总被引:2,自引:0,他引:2  
从土壤中筛选分离到1株γ-聚谷氨酸的生产菌株yt102,初步鉴定为枯草芽孢杆菌;以此为出发菌株采用紫外线(uV)、亚硝基胍(NTG)进行复合诱变,获得1株γ-聚谷氨酸高产突变株,突变株连续传代10次,发酵性能稳定;通过单因素和正交试验确定培养基的最佳组成,在最优条件下,γ-聚谷氨酸的平均产量可达28.5g/L。  相似文献
4.
Krüppel-like factor 17 (KLF17), a new member of the Krüppel-like factors (KLFs), has been reported to be a negative regulator of epithelial-mesenchymal transition (EMT) and metastasis in breast cancer. However, the biological role and clinical significance of KLF17 in lung adenocarcinoma has been less clear. In the present study, we showed that KLF17 expression was decreased in lung adenocarcinoma. Reduced expression of KLF17 was correlated significantly with a short survival time in patients with lung adenocarcinoma (P<0.0001). Moreover, KLF17 expression was an independent prognostic indicator for patients with lung adenocarcinoma. KLF17 expression level was correlated with the tumor stage (P=0.016) and tumor size (P=0.001) in lung adenocarcinoma. Overexpression of KLF17 inhibited cell growth in A549 and PC-9 cell lines. In conclusion, it is possible that KLF17 inhibits tumor growth in lung adenocarcinoma. The reduced expression of KLF17 is a valuable prognostic indicator for patients with lung adenocarcinoma, and KLF17 could be a novel target for treatment of lung adenocarcinoma.  相似文献
5.
6.
Impaired wound healing is a major diabetes-related complication. Keratinocytes play an important role in wound healing. Multiple factors have been proposed that can induce dysfunction in keratinocytes. The focus of present research is at a more specific molecular level. We investigated the role of advanced oxidative protein products (AOPPs) in inducing human immortalized keratinocyte (HaCaT) cell apoptosis and the cellular mechanism underlying the proapoptotic effect of AOPPs. HaCaT cells were treated with increasing concentrations of AOPP–human serum albumin or for increasing time durations. The cell viability was measured using the thiazolyl blue tetrazolium bromide method, and flow cytometry was used to assess the rate of cell apoptosis. A loss of mitochondrial membrane potential (MMP) and an increase in intracellular reactive oxygen species (ROS) were observed through a confocal laser scanning microscope system, and the level of ROS generation was determined using a microplate reader. Nicotinamide adenine dinucleotide phosphate oxidase (NOX)4, extracellular signal–regulated kinase (ERK)1/2, p38 mitogen-activated protein kinase (MAPK), and apoptosis-related downstream protein interactions were investigated using the Western blot analysis. We found that AOPPs triggered HaCaT cell apoptosis and MMP loss. After AOPP treatment, intracellular ROS generation increased in a time- and dose-dependent manner. Proapoptotic proteins, such as Bax, caspase 9/caspase 3, and poly(ADP-ribose) polymerase (PARP)-1 were activated, whereas anti-apoptotic Bcl-2 protein was downregulated. AOPPs also increased NOX4, ERK1/2, and p38 MAPK expression. Taken together, these findings suggest that extracellular AOPP accumulation triggered NOX-dependent ROS production, which activated ERK1/2 and p38 MAPK, and induced HaCaT cell apoptosis by activating caspase 3 and PARP-1.  相似文献
1
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号