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1.
人体外周血淋巴细胞核损伤指标的比较研究   总被引:16,自引:1,他引:15  
薛开先  王苏 《遗传学报》1990,17(1):70-74
核异常作为组织特异性的遗传毒理体内短期检测法,现已日益受到重视。它比微核测试更敏感和合理。核异常包括多种形式的核损伤,而它们与致癌因子的损伤有着不同程度的相关性。本文以r-射线作为致癌与诱变因子,在人体外周血淋巴细胞中系统地比较研究了常用核损伤指标:微核、核变形、核碎裂和核固缩等的剂量(0-5Grag)一反应关系,并作线形回归分析。作者认为,作为人体淋巴细胞核异常测试法,应包括微核,核变形及核碎裂3个核损伤指标。  相似文献
2.
广州市酸雨成分及其相关分析   总被引:13,自引:0,他引:13  
分析了2003年11月-2004年10月广州市龙洞79次降雨的化学组分及其各组分之间的相关关系。得出:(1) 观测期间总降雨量1359.8,单次降雨的pH值在3.22-7.29之间,平均4.83;以pH<5.6为酸雨的临界值,则酸雨频率占降雨次数的77.2%2或占降雨量的81.5%;(2)广州降水的化学组成以SO42-、Cl-和NO3-为主要阴离子,阳离子中以Ca2+、NH4+、Na+所占比例较大;(3)广州地区酸雨属硫酸型,降雨中的SO42-、NO3-、PO43-、Cl-、NH4+、K+、Na+、Ca2+、Mg2+ 离子浓度分别为0.150,0.045,0.002,0.163,0.058,0.006,0.013,0.083和0.007 mol·L-1,单次降雨的SO42-/NO3-比值在0.42- 17.6之间,年平均为3.33,该值比西南地区相应值低许多,而且与同一地点5年前测定的结果相比较,SO42-/NO3-比值有变小的趋势,说明广州地区NOx排放量高逐渐增加;(4)广州地区酸雨有着明显的季节变化规律,春、夏、秋季酸雨比较严重,冬季酸雨较少出现,这与西南地区的重庆(冬季酸雨较春季严重)酸雨季节规律相反;(5)通过SPSS统计分析得出,雨量与各化学组分含量成负相关,说明雨量对大气污染物质的稀释作用;酸雨各化学成分之间存在着一定的相关性,如SO42-与Ca2+,NO3-与NH4+,K+与Na+、Mg2+离子,Na+与Mg2+等都存在显著的正相关,利用这种相关可以建立某些酸雨成分的预测模型。  相似文献
3.
 层粘连蛋白(Laminin,LN)是基膜(basement membrane)中的一种主要大分子糖蛋白。一些研究资料表明肿瘤细胞的浸润转移可能与LN有关。肿瘤细胞与LN的作用可能是通过细胞表面LN受体进行的。本文采用亲和层析法从小鼠Lewis肺癌组织中分离LN受体并对其理化性质进行研究。Lewis肺癌LN受体的表观分子量为70,000,还原后SDS电泳图为一条较宽的条带。氨基酸组成中疏水氨基酸占38%,苏氨酸、絲氨酸、门冬氨酸(包括门冬酰胺)占23.5%,通过硝酸纤维素膜片法用HRP-LN测定受体与LN的结合特性,证明具有配基结合专一性,饱和性及高亲和性(Kd=0.95×10~(-9)mol/L)。  相似文献
4.
The development of axillary buds, terminal buds, and the shoots extended from them was studied inHydrangea macrophylla. The upper and lower parts in a nonflower-bearing shoot are discernible; the preformed part of a shoot develops into the lower part and the neoformed part into the upper part (Zhou and Hare, 1988). These two part are formed by the different degrees of internode elongation at early and late phases during a growth season, respectively. Leaf pairs in the neoformed part of the shoot are initiated successively with a plastochron of 5–20 days after the bud burst in spring. The upper axillary buds are initiated at approximately the same intervals as those of leaf pairs, but 10–30 days later than their subtending leaves. Changes in numbers of leaf pairs and in lengths of successive axillary buds show a pattern similar to the changes in internode lengths of the shoot at the mature stage. The uppermost axillary buds of the flower-bearing shoot often begin extending into new lateral shoots when the flowering phase has ended. The secondary buds in terminal and lower axillary buds are initiated and developed in succession during the late phase of the growth season. Internode elongation seems to be important in determining the degrees of development of the axillary buds. Pattern of shoot elongation is suggested to be relatively primitive. Significances of apical dominance and environmental conditions to shoot development are discussed.  相似文献
5.
 用电子显微镜观察了双翅目,无缝组,食蚜蝇科麻眼尾蛆蝇成熟精子的超微构造。麻眼尾蛆蝇的精子与果蝇的精子基本相似,呈线形,由头部和尾部组成,全长500—600微米。头部呈圆锥型,前端为单层结构的顶体,呈楔形插到核的侧面与核拼接。精子的尾部在头的后方,前粗后细,长500余微米。精子尾部内的微管排列为9+9+2的典型昆虫型。头部与尾部相接之处称为颈部,组合情况颇为复杂。本种精子尾的轴丝不直接与核相接,而是轴丝串联着基体(中心粒衍生物)隔着一层中心粒侧体与核相接。这种间接连接现象在果蝇未成熟的精子中可以见到,当精子成熟时中心粒侧体即行消失,核就直接与串联着基体的轴丝直接相接。麻眼尾蛆蝇成熟精子具有其他蝇类未成熟精子的性状,故可认为麻眼尾蛆蝇在蝇类系统发生中的地位较原始。  相似文献
6.
Characteristics of the hyperhydric protocormlike-bodies (hPLBs) and the normal PLBs (nPLBs) of Doritaenopsis are morphologically and ontogenetically compared. The hPLBs have a translucent and turgid appearance which is due to a lack of air volume in the intercellular spaces. The hPLBs have a lower capacity of shoot formation but a higher capacity of differentiation of new PLBs than the nPLBs. The new PLBs derived from the hPLBs can be recovered from hyperhydricity using a medium containing potato juice. This suggests the possibility that hPLBs can be used for the in vitro propagation of orchid plants.Abbreviations PLB Protocorm-Like-Body - mVW Modified Vacin and Went Medium  相似文献
7.
The structure of shoots, in particular of winter buds, ofHydrangea macrophylla was examined. The non-flower-bearing shoot is usually composed of a lower and an upper part, between which a boundary is discernible by means of a distinctly short internode. This internode is the lowermost of the upper part, and it is usually shorter than the internodes immediately above and below, although the internodes tend to shorten successively from the proximal to the distal part of the shoot. Variations exist in the following characters among the terminal bud, the axillary bud on the lower part of the shoot and the axillary bud on the upper part: (1) length of bud; (2) character of the outermost pair of leaf primordia; (3) degree of development of secondary buds in the winter bud; and (4) the number of leaf primordia. Usually, the terminal bud contains several pairs of foliage leaf primordia with a primordial inflorescence at the terminal of the bud, but the axiallary bud contains only the primordia of foliage leaves in addition to a pair of bud scales.  相似文献
8.
A high-density map consisting of 550 markers was constructed based on the segregation data of 95 doubled-haploid lines (DHLs) derived from the cross between a Japanese barley cultivar, Mikamo Golden and a North American barley cultivar, Harrington (MH-DHLs). Quality traits of malt extract (EX), total nitrogen (TN), soluble nitrogen (SN), Kolbach index (KI), diastatic power (DP), wort beta-glucan (WG) and viscosity (VS) were determined in three site/year crops. Quantitative trait loci (QTL) analyses were performed with these quality data sets, using the linkage map. Major QTL controlling EX, SN and KI were mapped on terminal region of 5H with Harrington as effective allele. Another QTL controlling EX was mapped on 2H with Mikamo Golden as effective allele. QTL controlling TN, DP, WG and VS were detected variably in terms of flanking markers and chromosomes depending on site/year. Cleaved amplified polymorphic sequences (CAPS) markers for EX based on the QTL detected on 2H and 5H were developed. Analysis of EX and genotypes of 33 malting barley cultivars from around the world as well as MH-DHLs revealed that the two CAPS marker on 2H and 5H affect EX by a significant difference, suggesting that the two CAPS markers were valuable for marker-assisted selection in malting barley breeding.  相似文献
9.
目的:分析转基因c-Ha-ras在C57-ras癌症小鼠模型中的组织表达谱及时空表达差异。方法:利用半定量及荧光定量RT-PCR法,分析不同首建鼠系、不同周龄小鼠各脏器中转基因c-Ha-ras的表达。结果:转基因c-Ha-ras在心、肝等13种组织器官中均有表达,在肺脏中表达最高,在肝脏中表达最低,No.2、No.3和No.5等3个首建鼠均呈现相似的变化规律;转基因在No.5首建鼠中表达水平最高,而在同一个首建鼠系中,12周龄时表达高于8周龄和24周龄。结论:转基因c-Ha-ras在各脏器中能高效表达,并间接表明该转基因能稳定遗传,为C57-ras癌症小鼠模型用于新药临床前致癌性评价提了供理论支持。  相似文献
10.
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