排序方式: 共有12条查询结果,搜索用时 46 毫秒
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利用RTPCR技术合成并扩增了水稻条叶枯病毒(RStV)中国云南分离物基因组组分4的全长cDNA,将PCR产物克隆在载体pCRII上,并进行全序列测定,所得核苷酸序列及推测的氨基酸序列与日本分离物T进行比较。结果表明,在核苷酸水平,两分离物的vORF、vcORF及基因间非编码区序列的同源性分别为94.9%、94.1%、86.1%,5’端非编码区序列相同,而3’非编码区同源性为96.1%,仅有两个核苷酸不同;在氨基酸水平,vORF及vcORF编码蛋白的同源性分别为99.4%和98.3%。可见,编码区的大小及其氨基酸序列和两末端序列都是很保守的。因此,中国云南分离物Y与日本分离物T可能有很近的亲缘关系。 相似文献
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Itisaworld-widefocusthatsoilbecomesmoreandmoresaline.Aboutone-thirdirrigablelandbecomeslostallovertheworldforthereasonthatthesalinityistoohigh.Therefore,im-provingthesalt-toleranceofcropsseemsmoreandmoreimportant.Previousstudyonthemecha-nismofsaltresistantofplantshaspaidmoreattentiontohigherplants,whileonlyafewtoalgae.Studyintheseyearsshowedthatalgaehadthesimilarbiophysiologicalandbiochemicalreactiontoallhigherplantsinadversity[1].Soitisreliabletoapplyalgatorelatedstudy.Dunaliellasalina,akind… 相似文献
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沃尔巴克氏体在中国三种稻飞虱中的感染 总被引:12,自引:3,他引:9
用PCR方法检测了采集于不同地域稻田的3种稻飞虱共生菌沃尔巴克氏体(Wolbachia)的感染,发现灰飞虱Laodelphax striatellus、褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera为沃尔巴克氏体所感染。克隆了编码沃尔巴克氏体外膜蛋白质的wsp基因并进行了序列测定。对wsp的RFLP分析证实了这些飞虱为单一沃尔巴克氏体感染。研究了灰飞虱中沃尔巴克氏体所诱导的胞质不相容性及其在不同地域灰飞虱中的分布。还发现能寄生于上述3种飞虱的稻虱红螯蜂也受同种沃尔巴克氏体感染。沃尔巴克氏体可能通过这种寄生蜂在不同昆虫间横向传播。 相似文献
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受NaCl诱导的盐藻果糖-1,6-二磷酸醛缩酶cDNA的克隆及其在烟草中的表达 总被引:8,自引:0,他引:8
采用快速扩增cDNA末端(RACE)技术, 获得了盐藻(Dunaliella salina)受诱导表达的果糖-1,6-二磷酸醛缩酶全长cDNA (DsALDP). 蛋白质序列分析发现, DsALDP与许多植物叶绿体的果糖-1,6-二磷酸醛缩酶(AldP)具有较高的同源性(66%~73%). 系统进化分析进一步证明DsALDP与藻类的AldP亲缘关系最近. 就表达谱而言, DsALDP是NaCl诱导下新表达的转录本, 其表达水平随诱导时间的不同而呈显著变化. 筛选的DsALDP cDNA构建到双元载体pBI121并导入农杆菌用以转化烟草, 对4个T1代转基因植株进行Southern检测, 证明DsALDP已整合入转基因植株的基因组. RT-PCR分析显示DsALDP在这些植株中均得到有效表达. 生物测试表明, T1-1, T1-2和T1-3植株在100~200 mmol/L NaCl下仍保持醛缩酶活性, 且其脯氨酸含量均有不同程度的升高. 相似文献
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水稻磷酸盐转运蛋白基因的克隆、表达及功能分析 总被引:5,自引:0,他引:5
以水稻叶片为材料, 设计一对特异引物, 获得了编码磷酸盐转运蛋白基因OsPT6:1. 聚类和氨基酸保守位点分析指出该基因可能为水稻高亲和力磷酸盐转运蛋白编码基因. 原位杂交与RT-PCR表达结果确定此基因在根与叶片中均表达, 尤以低磷诱导下叶片的叶肉细胞表达量最高. 同源重组表明该基因的表达可以提高毕氏酵母对磷素的吸收效率, 同时其基因的导入可以使高亲和力磷酸盐转运蛋白缺失的酵母突变体的磷素吸收功能得以恢复. 以上结果表明, OsPT6:1为水稻高亲和力磷酸盐转运蛋白的编码基因. 相似文献
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Using Rapid Amplification of cDNA ends (RACE) technique, the full-length cDNA encoding a NaCl-induced fructose-1, 6-diphosphate
aldolase (DsALDP) was obtained. It was shown that the DsALDP had a relatively high homology (66%–73%) to chloroplast fructose-1, 6-diphosphate
aldolase (AldP) in many plants according to their amino acid sequences. The phylogenetic analysis further confirmed that AldP
in alga is the nearest to DsALDP. As to its expression pattern,DsALDP was denovo synthesized by NaCl induction. Its expression level was significantly changed with inducing time. After the selectedDsALDP cDNA subcloned into a binary vector pBI121, the new construct was introduced into tobacco byAgrobacterium tumefaciens. The results of Southern blot and RT-PCR analysis of four transgenic T1 plants indicated thatDsALDP was integrated into genome of these transgenic plants and effectively expressed. Aldolase activities have been detected in
T1-1, T1-2 and T1-3 plants by bioassay under 100–200 mmol/L NaCl. It was also observed that proline contents in them were
differentially increased. 相似文献