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981.
Sun M 《American journal of botany》1997,84(2):224-232
Comparative studies on the genetic attributes of colonizing orchids with diverse mating systems are lacking in the literature. Eulophia sinensis, Spiranthes hongkongensis, and Zeuxine strateumatica are colonizing orchids that frequently occupy newly created habitats in Hong Kong. Mating system studies showed that E. sinensis is a self-compatible but pollinator-dependent outcrossing species, S. hongkongensis is a self-pollinating taxon, and Z srateumatica is apomictic. Population genetic attributes of these orchid species were investigated. Despite their contrasting mating systems, electrophoretic surveys revealed a striking lack of allozyme variation, both within and among populations, in all three species. However, gene duplications were evident in these species, due to their likely polyploid origins, The percentage of duplicate loci exhibiting "fixed heterozygosity" was 10, 21.4, and 20% in E. sinensis, S. hongkongensis, and Z. strateumatica, respectively. The genetic attributes of these orchids are compared to those found in other colonizing plant species. 相似文献
982.
We investigated the scale of genetic variation of purple needlegrass (Nassella pulchra), a species commonly used in California for grassland restoration. Common garden and field data revealed evidence of genetic
differentiation between two intermixed microhabitats characterized by differences in soil depth and community composition.
We assessed the genetic variation within a single population using randomly amplified polymorphic DNA (RAPD) data collected
from clusters of five individuals in 40 locations. We found no evidence for genetic structure at the whole population level.
At smaller spatial scales, however, we found strong evidence that genetic subdivision of the population occurs at the level
of the maternal neighborhood. We suggest that the interaction between widespread pollen dispersal and restricted seed dispersal
may be the primary factor generating these results; panmictic pollen dispersal will make detection of genetic patterning difficult
at larger spatial scales while limited seed dispersal will generate local genetic structure. As a result, the detection of
population genetic structure will depend on the spatial scale of analysis. Local selection gradients related to topography
and soil depth are also likely to play a role in structuring local genetic variation. Since N. pulchra is widely used in California in grassland and woodland habitat restoration, we suggest that, as a general rule, care should
be exercised in transferring germplasm for the purposes of conservation when little is known about the within-population genetic
subdivision of a plant species.
Received: 23 December 1996 / Accepted: 20 May 1997 相似文献
983.
Public informatics resources for rice and other grasses 总被引:1,自引:0,他引:1
As an emerging model system, rice will benefit from an informatics infrastructure which organizes genome data and makes it available worldwide. RiceGenes and other Internet-accessible resources are evolving to meet these goals. Grass crops such as rice, maize, millet, sorghum and wheat are closely related but are represented by independent database projects; interlinking these resources would create a broad view of grass genetics and make it easier to compare data across genomes. The future success of grass informatics depends on the development of new comparative mapping displays as well as the participation of the research community in assembling and curating comparative map data. 相似文献
984.
MARTIN G. RAMIREZ B. CHI 《Biological journal of the Linnean Society. Linnean Society of London》2004,82(1):27-37
Populations of the turret spider Atypoides riversi from eight central Californian sites were compared based on variation at ten allozyme loci. Multidimensional scaling of interpopulation genetic distances defined four population units (Coast Range, Sierran, Valley, Jenness Camp), corroborating the distinctness of Coast Range and Sierran populations indicated by a prior study. While the species status of these units has yet to be determined, Jenness Camp is the most likely to represent a new species, given its clear genetic uniqueness (two fixed allelic differences). Populations in all units were generally in Hardy–Weinberg equilibrium with no evidence of inbreeding, though variability was minimal (mean H o = 2.8%, mean P = 15.4%). Reduced variability in these populations may be the result of repeated bottlenecks, environmental homogeneity, and/or directional selection. Interpopulation differentiation within units was significant in the absence of intervening forest habitat and was substantially less in its presence, indicating that gene flow is likely only when forest corridors exist. To foster preservation of the existing gene pool and enhance participation in it, management of the units of At. riversi should focus on maintaining as many populations in situ as possible and facilitating connections between them, while also creating or restoring habitat for potential colonization. © 2004 The Linnean Society of London, Biological Journal of the Linnean Society , 2004, 82 , 27–37. 相似文献
985.
Katharine M. Evans Stephen S. Bates Linda K. Medlin Paul K. Hayes 《Journal of phycology》2004,40(5):911-920
The genetic structure of phytoplankton populations is largely unknown. In this study we developed nine polymorphic microsatellite markers for the domoic acid–producing marine diatom Pseudo‐nitzschia multiseries (Hasle) Hasle. We then used them in the genotyping of 25 physiologically diverse field isolates and six of their descendants: 22 field isolates originated from eastern Canadian waters, two from European waters, and one from Russian waters. The numbers of alleles per locus ranged from three to seven and the observed heterozygosities from 0.39 to 0.70. A substantial degree of genetic variation was observed within the field isolates, with 23 different genotypes detected. The Russian isolate was the most genetically distinct, although there was also evidence of genetic differentiation at a more local scale. Mating experiments demonstrated that alleles were inherited in a Mendelian manner. Pseudo‐nitzschia multiseries primer pairs were tested on DNA from four congeners: P. calliantha Lundholm, Moestrup et Hasle; P. fraudulenta (P. T. Cleve) Hasle; P. pungens (Grunow ex P. T. Cleve) Hasle; and P. seriata (P. T. Cleve) H. Peragallo. Cross‐reactivity was only observed in P. pungens. Our results are a first step in understanding the genetic variation present at the Pseudo‐nitzschia“species” level and in determining the true biogeographic extent of Pseudo‐nitzschia species. 相似文献
986.
C. Nielsen G. Holdensgaard H. C. Petersen B. Th. Björnsson S. S. Madsen 《Journal of fish biology》2001,59(1):28-44
Out of five strains of Atlantic salmon Salmo salar of 1+ years released upstream of a fyke net in the River Gudenaa in 1996, three, Lagan, Ätran and Corrib, migrated immediately, 50% of the recaptured fish reaching the net in 3–6 days. Burrishoole and Conon fish migrated with a 15–19 day delay. Smolt development in 1997 at the hatchery showed a spring surge in gill Na+ , K+ -ATPase activity in all strains which was correlated with increased seawater tolerance. Differences in the timing of gill enzyme development matched the observed migration pattern well. Lagan, Ätran and Corrib strains reached high enzyme activity earlier than the Burrishoole and Conon strains, and strains with delayed enzyme development and migration showed a delayed regression of seawater tolerance compared with the early strains. Inter-strain differences in plasma growth hormone profiles could not be related to the observed patterns of Na+ , K+ -ATPase and seawater tolerance development. The study gives evidence of genetic influence on the timing and intensity of smolting and subsequent migration in Atlantic salmon. 相似文献
987.
MARCUS K. DROTZ 《Biological journal of the Linnean Society. Linnean Society of London》2003,79(4):653-666
Diversification of populations of two morphologically similar diving beetles within the Agabus tristis group, A. wollastoni and A. bipustulatus, was investigated, with partial mtDNA cytochrome b (Cyt b) sequences, allozymes and landmark‐based morphometrics. The Madeiran endemic A. wollastoni was collected from 11 localities. Population genetic and morphological variation was compared to Scandinavian localities of the widespread west Palearctic A. bipustulatus, recorded also from the Azores. Agabus wollastoni and European A. bipustulatus specimens representing eight and 13 localities respectively, were used in evaluating their phylogenetic relationship. Maximum parsimony analysis of the Cyt b sequences showed that both the A. bipustulatus and A. wollastoni specimens form well‐supported monophyletic groups. Three lines of evidence suggest that Agabus wollastoni has speciated through a few founders: (1) a well‐supported mtDNA line; (2) the mean heterozygosity of A. wollastoni is lower when compared to A. bipustulatus on the mainland; and (3) several uncommon alleles of A. bipustulatus are missing in A. wollastoni. The Azorean A. bipustulatus population was drastically affected by the colonization event, since several loci have become fixed with a resulting lower mean heterozygosity. The colonization was relatively recent, as the mtDNA lineage represented in the Azores is deeply nested within the A. bipustulatus clade. Population structure shows moderate inbreeding of A. wollastoni, and extensive substructuring at all localities with moderate gene‐flow between them. Morphological variation in A. wollastoni showed significant differentiation among several populations. Island colonizations, population structure of A. wollastoni, and an observed pattern of variation of the α‐glycerophosphate dehydrogenase locus are discussed. © 2003 The Linnean Society of London, Biological Journal of the Linnean Society, 2003, 79, 653–666. 相似文献
988.
CHRISTOPH REISCH PETER POSCHLOD RUTH WINGENDER 《Biological journal of the Linnean Society. Linnean Society of London》2003,80(1):11-21
Saxifraga paniculata Mill. is an amphi-atlantic species that is widely distributed in the Alps but also occurs with disjunct populations in central Europe. These isolated populations are considered to be glacial relicts. In the study presented here, we analysed the genetic variation within and between populations of Saxifraga paniculata in central Europe to test whether the molecular data give evidence for the supposed relict endemism. We used RAPD analysis and the analysis of molecular variance (AMOVA) technique to investigate 30 populations of Saxifraga paniculata from different locations in central Europe and adjacent geographical areas. In the RAPD analysis, 319 fragments could be amplified of which 91.2% were polymorphic. The percentage of polymorphic bands within populations correlated significantly with population size, indicating a higher level of inbreeding in small populations. AMOVA revealed 43.60% of the variation within populations, only 15.45% between populations within locations, and 40.95% between locations. Genetic distance between locations (ΦST ) correlated significantly with the geographical distance between locations, giving evidence for geographical isolation. In an UPGMA cluster analysis, based on RAPD data, the populations were grouped together according to their location. The results exhibit a strong genetic differentiation which is obviously due to genetic drift in the isolated populations. Our study therefore gives evidence for glacial relict endemism of Saxifraga paniculata in central Europe. © 2003 The Linnean Society of London, Biological Journal of the Linnean Society , 2003, 80, 11–21. 相似文献
989.
M. Wang G. Lang A. Schreiber 《Journal of Zoological Systematics and Evolutionary Research》2002,40(4):232-236
DNA‐microsatellite polymorphism (four loci) was studied in 56 male roe deer (Capreolus capreolus) from a 900‐ha hunting territory in the Vosges du Nord Mountains (France), culled over 34 years (1956–1990). Changed allele frequencies at two loci within this period, and increased allelic diversity, were traced to a phase of reduced population density and subsequent immigration. Decadic population samples collected within 900‐ha were distinguished by higher genetic variability measures than were certain geographical samples across Central Europe (4–900 km). On average, the decadic cohorts were distinguished by a gene diversity index of GST = 0.0286, and a genetic distance of D = 0.0938, which reflect 54% (GST) and 69% (D) of the respective geographic (350 km) differentiation indices of roe deer in Central Europe. The importance of demography and population ecology effects for microevolution in a large mammal is demonstrated, as is the risk of artefact by composing population samples of deer over several years. Population genetic screening should cover various demes of roe deer from the same general region, and be based on many unlinked polymorphic loci, to minimize the distorting effects of genetic dynamics at the small spatial scale. 相似文献
990.
John P. van der Meer 《Journal of phycology》1986,22(2):151-158
A spontaneous bisexual mutant of Gracilaria tikvahiae McLachlan has been further characterized. Female plants that are carriers for the mutation, but do not themselves express bisexuality, have been identified among progeny derived from the original bisexual male plant. In crosses to normal males these carrier females yielded normal tetrasporophytes which in the subsequent gametophyte generation produced a 2 female: 1 male: 1 bisexual segregation. In crosses to bisexual males the carrier females produced unusual tetrasporophytes that formed cystocarps in addition to tetraspores. The gametophyte generation obtained from the tetraspores of these tetrasporophytes included only females and bisexuals, these being present in a 1:1 ratio. Other crosses, using bisexual male progeny, indicated that these have the same characteristics as the original bisexual mutant. All of the results are consistent with the genetic interpretation made previously that bisexuality results from a single recessive mutation, designated bi, in a gene distinct from the mt locus controlling male vs. female differentiation. From the phenotypes that have been observed it appears that the mutation does not cause bisexuality per se but rather results in unregulated female expression in males and tetrasporophytes where female-specific genes are normally repressed. It is suggested that the normal bi+ allele plays an important role in that repression process. The origin of the bisexual mutation has been re-examined by studying the progenitor stock of the mutation and stocks related to it. It appears that the bisexual mutation arose in a two-step process, first to a low level of expression that is found in the progenitor stocks and then to the high level of expression found in mutant clone 1045(bi) and its descendants. 相似文献