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排序方式: 共有213条查询结果,搜索用时 25 毫秒
81.
Tariq M. Bhatti Jerry M. Bigham Liisa Carlson Olli H. Tuovinen 《Applied microbiology》1993,59(6):1984-1990
The biological leaching of pyrrhotite (Fe1-xS) by Thiobacillus ferrooxidans was studied to characterize the oxidation process and to identify the mineral weathering products. The process was biphasic in that an initial phase of acid consumption and decrease in redox potential was followed by an acid-producing phase and an increase in redox potential. Elemental S was one of the first products of pyrrhotite degradation detected by X-ray diffraction. Pyrrhotite oxidation also yielded K-jarosite [KFe3(SO4)2(OH)6], goethite (α-FeOOH), and schwertmannite [Fe8O8(OH)6SO4] as solid-phase products. Pyrrhotite was mostly depleted after 14 days, whereas impurities in the form of pyrite (cubic FeS2) and marcasite (orthorhombic FeS2) accumulated in the leach residue. 相似文献
82.
An improved solid medium for isolation,enumeration and genetic investigations of autotrophic iron-and sulphur-oxidizing bacteria 总被引:1,自引:0,他引:1
An improved solid medium using Gelrite as a supporting gel for isolation and enumeration of acidophilic chemolithotrophic Thiobacillus strains was tested. Dark-brown circular colonies, which were robust and well-differentiated, developed on this medium within 72–96 h. The plating efficiency of T. ferrooxidans strains was 92.1±5.8%. Linear correlations with an optical density (OD) at 400 nm of 0.5, corresponding to 7·106 and 5·107 cells·ml–1 for strains ATCC 13661 and TMB, respectively, were obtained. Linear correlations between OD, total bacterial protein, dry biomass and cell number were also determined. These data can be used for modelling and scale-up design of microbial leaching operations. With this plating technique, one prerequisite for selection of different mutants of T. ferrooxidans has been fulfilled.
Correspondence to: A. M. Khalid 相似文献
83.
Separation and partial characterization of two types of alkaline phosphatase from Serratia marcescens (Short Communication) 下载免费PDF全文
A. R. Bhatti 《The Biochemical journal》1973,135(3):537-538
The purification and separation of two electrophoretically distinct and chemically different alkaline phosphatases from Serratia marcescens is described. 相似文献
84.
Cell division in Pseudomonas aeruginosa: participation of alkaline phosphatase. 总被引:5,自引:2,他引:3 下载免费PDF全文
Pseudomonas aeruginosa grows at an apparent reduced rate at 46 C as compared with the rate at 37 C, when growth is measured as an increase in absorbance. Cells at 46 C are long, plasmolyzed, nonmotile filaments. The filaments contain phase-dark material that may be chromosomal in nature. When the 46 C culture is shifted to 37 C, the filaments fragment at polar ends after flagella form, and the final number of cells is equal to the number of chromosomal "packets" observed within the filament. The outer envelope of the filament appears to be structurally complete as determined by biochemical, thin section, and freeze-etch examination. When filaments are treated with lysozyme, they form large spheroplasts, suggesting that the outer wall and the cytoplasmic membrane are continuous within the filament. Filaments produce little or no periplasm-located alkaline phosphatase (APase), but activity appears immediately after a shift to 37 C. Cells grown at 37 C and shifted to 46 C remain as single, nonmotile, rods or doublets, and the APase formed at 37 C remains stable at 46 C. The addition of APase or inorganic phosphate is partially or completely effective as an inducer of filament fragmentation at 46 C. The results suggest that periplasm-located APase is an important enzyme in the final stages of cell division when P. aeruginosa is cultured on inorganic phosphate-limiting media. 相似文献
85.
B A Magnuson H B Schiefer D S Hancock A R Bhatti 《Canadian journal of physiology and pharmacology》1987,65(5):799-802
Evidence has been mounting that trichothecenes cause cardiac lesions and cardiovascular effects in general. T-2 toxin, dissolved in dimethyl sulfoxide, was applied in doses of 0, 1.0, 2.0 mg/kg to the skin of Sprague-Dawley rats. Twenty-four hours later, the cardiac function of the animals was assessed, followed by killing and histological examination. It was found that the arterial blood pressure values were lower in the 2.0 mg/kg group, the peak intraventricular pressure was lower in both the 1.0 and 2.0 mg/kg groups, and the resting systolic and diastolic blood pressure values of the 2.0 mg/kg group were lower than the 0 and 1.0 mg/kg groups. The 1.0 and 2.0 mg/kg groups had significantly lower epinephrine-stimulated intraventricular pressure values, indicating reduced contractility. Extended Q-T intervals in electrocardiograms of the 1.0 and 2.0 mg/kg groups suggested also impaired contractility. The histological examination gave equivocal results. It is concluded that topical applications of small doses of T-2 toxin have a noticeable negative effect on cardiovascular function. 相似文献
86.
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88.
Review of Epidemiology, Diagnosis, and Treatment of Invasive Mould Infections in Allogeneic Hematopoietic Stem Cell Transplant Recipients 总被引:8,自引:0,他引:8
Invasive mould infections are a major cause of morbidity and mortality in hematopoietic stem cell transplant recipients (HSCT).
Allogeneic HSCT recipients are at substantially higher risk than autologous HSCT recipients. Although neutropenia following
the conditioning regimen remains an important risk factor for opportunistic fungal infections, most cases of invasive mould
infection in allogeneic HSCT recipients occur after neutrophil recovery in the setting of potent immunosuppressive therapy
for graft-versus-host disease. Invasive aspergillosis is the most common mould infection. However, there has been an increased
incidence of less common non-Aspergillus moulds that include zygomycetes, Fusarium sp., and Scedosporium sp. Reflecting a key need, important advances have been made in the antifungal armamentarium. Voriconazole has become a new
standard of care as primary therapy for invasive aspergillosis based on superiority over amphotericin B. There is significant
interest in combination therapy for invasive aspergillosis pairing voriconazole or an amphotericin B formulation with an echinocandin.
There have also been advances in novel diagnostic methods that facilitate early detection of invasive fungal infections that
include galactomannan and beta-glucan antigen detection and PCR using fungal specific primers. We review the epidemiology,
diagnosis, and management of invasive mould infection in HSCT, with a focus on allogeneic recipients. We also discuss options
for prevention and early treatment of invasive mould infections. 相似文献
89.
90.
Brahm H. Segal Wei Han Jennifer J. Bushey Myungsoo Joo Zahida Bhatti Joy Feminella Carly G. Dennis R. Robert Vethanayagam Fiona E. Yull Maegan Capitano Paul K. Wallace Hans Minderman John W. Christman Michael B. Sporn Jefferson Chan Donald C. Vinh Steven M. Holland Luigina R. Romani Sarah L. Gaffen Michael L. Freeman Timothy S. Blackwell 《PloS one》2010,5(3)