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721.
Yanyan Zhao Ying Liang Yuan Liu Xiao Zhang Xiaodan Hu Sicong Tu Aihua Wu Cunzheng Zhang Jianfeng Zhong Shengming Zhao Xianjin Liu Kang Tu 《Analytical biochemistry》2016
An antibody to phenoxybenzoic acid (PBA), the conserved chemical region of pyrethroids, was developed using a domain antibody (DAB) library to enable pyrethroid detection in agricultural products. The DAB library, constructed without animal immunization and based on a human VH framework, displayed repertoires on filamentous bacteriophage. After four rounds of panning, we obtained five domain antibodies that are capable of binding to PBA. Antibody A3 has strong identification capability to cypermethrin, β-cypermethrin, and fenvalerate. The antibody A3 was used to develop an enzyme-linked immunosorbent assay (ELISA). The IC50 values were 2.586, 1.814, and 2.251 μg/ml for cypermethrin, β-cypermethrin, and fenvalerate, respectively. The assay shows weak competition with flucythrinate but shows no competition with fenpropathrin, deltamethrin, and permethrin. The developed ELISA process was successfully applied to fortified Chinese cabbage samples, with the recoveries of cypermethrin, β-cypermethrin, and fenvalerate ranging from 84.4 to 112.3%. We developed an immunoassay to detect pyrethroids depending on the domain antibody library, which overcomes the limitation of requiring protein antigen to immunize animals raising antibody. 相似文献
722.
Andrew W. Munro Hazel M. GirvanKirsty J. McLean 《Biochimica et Biophysica Acta (BBA)/General Subjects》2007
The cytochromes P450 (P450s) are a broad class of heme b-containing mono-oxygenase enzymes. The vast majority of P450s catalyse reductive scission of molecular oxygen using electrons usually derived from coenzymes (NADH and NADPH) and delivered from redox partner proteins. Evolutionary advantages may be gained by fusion of one or more redox partners to the P450 enzyme in terms of e.g. catalytic efficiency. This route was taken by the well characterized flavocytochrome P450BM3 system (CYP102A1) from Bacillus megaterium, in which soluble P450 and cytochrome P450 reductase enzymes are covalently linked to produce a highly efficient electron transport system for oxygenation of fatty acids and related molecules. However, genome analysis and ongoing enzyme characterization has revealed that there are a number of other novel classes of P450–redox partner fusion enzymes distributed widely in prokaryotes and eukaryotes. This review examines our current state of knowledge of the diversity of these fusion proteins and explores their structural composition and evolutionary origins. 相似文献
723.
Kotaro T. Yamamoto 《Journal of plant research》1990,103(4):469-491
Phytochrome is a proteinaceous pigment that acts as a photoreceptor for photomorphogenetic responses in plants. It exists
as two stable absorbing forms, Pr and Pfr, which are interconvertible reversibly by irradiation with red or far-red light. The present review discusses (i) the primary
and higher-order structures of phytochrome that permit the reversible photoreaction; (ii) the molecular properties which change
accompanying the phototransformation; and (iii) the four-leaved shape model which has recently been proposed as a model of
quaternary structure of phytochrome. 相似文献