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511.
Zongtao Sun Shaochuan Shen Bing Tian Hu Wang Zhenjian Xu Liangyan Wang & Yuejin Hua 《FEMS microbiology letters》2009,301(1):21-27
Deinococcus radiodurans strain R1 synthesizes a unique ketocarotenoid product named deinoxanthin. The detailed steps involved in the biosynthesis of deinoxanthin remain unresolved. A carotene ketolase homologue encoded by dr0093 was inactivated by gene mutation to verify its function in the native host D. radiodurans . Analysis of the carotenoids in the resultant mutant R1ΔcrtO demonstrated that dr0093 encodes γ-carotene ketolase (CrtO) catalysing the introduction of one keto group into the C-4 position of γ-carotene derivatives to form ketolated carotenoids. The mutant R1ΔcrtO became more sensitive to H2 O2 treatment than the wild-type strain R1, indicating that the C-4 keto group is important for the antioxidant activity of carotenoids in D. radiodurans . Carotenoid extracts from mutant R1ΔcrtO exhibited lower 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity than those from the wild-type strain R1. The enhanced antioxidant ability of ketocarotenoids in D. radiodurans might be attributed to its extended conjugated double bonds and relative stability by the C-4 keto group substitution. 相似文献
512.
Headspace ethylene accumulation effects on secondary metabolite production in Vaccinium pahalae cell culture 总被引:1,自引:0,他引:1
The influence of headspace ethylene on anthocyanin, anthocyanidin, and carotenoid accumulation was studied in suspension cultures of Vaccinium pahalae. Exogenous application of ethrel (an ethylene-releasing compound) significantly reduced growth and secondary metabolite production, whereas incorporation of 5.0 or 10.0 mg l-1 CoCl2 or NiCl2 effectively reduced ethylene accumulation and improved product accumulation, but AgNO3was toxic to cells. This study showed an overall negative impact of increased ethylene levels in the vessel headspace on phytochemical production in ohelo cell cultures. 相似文献
513.
514.
F.C. Pennington F.T. Haxo G. Borch S. Liaaen-Jensen 《Biochemical Systematics and Ecology》1985,13(3):215-219
The carotenoids of selected Cryptophyceae, Rhodomonas D3 and Cryptomonas ovata, have been examined by methods including HPLC, mass spectrometry 1H NMR and circular dichroism. has been assigned to monadoxanthin from 1H NMR and CD data; β,?-carotene possessed the common . The quantitative distribution pattern of carotenoids in Cryptophyceae established here and previously, totalling five species' is discussed in chemosystematic context. β,?-Carotene (3–8% of total) is the major carotene, accompanied by ?,?-carotene (0.2%), β,β-carotene (0–1%) and lycopene (0-trace). Zeaxanthin (2%) was identified in C. ovate. The diacetylenic alloxanthin is the major carotenoid (70–88%), and the monoacetylenic crocoxanthin (5–15%) and monadoxanthin (0–16%) less abundant. No epoxidic or allenic carotenoids could be detected. The biosynthetic precursor of acetylenic carotenoids in this primitive algal class is discussed. The significance of Cryptophyceae in the marine food chain is commented on, using alloxanthin as an indicator. 相似文献
515.
Forty-seven species of desmids, representing all four families, were examined for the presence of the xanthophyll loroxanthin by reverse-phase high-performance liquid chromatography. In the Desmidiaceae 28 of the 35 species examined possessed loroxanthin, and in the Mesotaeniaceae two of the six examined had loroxanthin present. All six species of the families Peniaceae and Closteriaceae examined possessed loroxanthin. Although the distribution of loroxanthin appears to be disjunct in the desmids and does not have strict taxonomic significance, it does follow a coherent pattern consistent with current ideas on desmid phylogeny. This pattern suggests that loroxanthin synthesis probably evolved once in the desmid lineage, with one or more subsequent reversals. 相似文献